Purification and characterization of a ginsenoside Rb1-hydrolyzing β-glucosidase from Aspergillus niger KCCM 11239

International Journal of Molecular Sciences

Volumn 13, Issue 9, 2012, Pages 12140-12152

  Chang, Kyung Hoon ^a   Jo, Mi Na ^a   Kim, Kee Tae ^a   Paik, Hyun Dong ^a  

^a Konkuk University   (South Korea)

Author keywords

Aspergillus niger KCCM 11239; Ginsenoside Rd; glucosidase

Indexed keywords

BETA GLUCOSIDASE; GINSENOSIDE RB 1; GINSENOSIDE RB 2; GINSENOSIDE RC; GINSENOSIDE RD; GINSENOSIDE RE; GINSENOSIDE RG 1;

ARTICLE; ASPERGILLUS NIGER; BIOTRANSFORMATION; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SYNTHESIS; FUNGAL STRAIN; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; MOLECULAR WEIGHT; NONHUMAN; PH; TEMPERATURE SENSITIVITY;

ASPERGILLUS NIGER; PANAX GINSENG;

EID: 84866923150     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms130912140     Document Type: Article

References (31)

1. Pitson, S.M.; Seviour, R.J.; McDougall, B.M. Purification and characterization of an extracellular β-glucosidase from the filamentous fungus Acremonium persicinum and its probable role in β-glucan degradation. Enzyme Microb. Technol. 1997, 21, 182-190.
2. Hsieh, M.C.; Graham, T.L. Partial purification and characterization of a soybean β-glucosidase with high specific activity towards isoflavone conjugates. Phytochemistry 2001, 58, 995-1005.
3. Esen, A. β-Glucosidases: Overview. In β-Glucosidases-Biochemistry and Molecular Biology; American Chemical Society: Washington DC, USA, 1993; pp. 1-14.
4. Su, J.H.; Xu, J.H.; Yu, H.L.; He, Y.C.; Lu, W.Y.; Lin, G.Q. Properties of a novel β-glucosidase from Fusarium proliferatum ECU2042 that converts ginsenoside Rg3 into Rh2. J. Mol. Catal. B 2009, 57, 278-283.
5. Terra, W.R.; Ferreira, C. Insect digestive enzymes: Properties, compartmentalization and function. Comp. Biochem. Physiol. B 1994, 109, 1-62.
6. Dan, S.; Marton, I.; Dekel, M.; Bravdo, B.; He, S.; Withers, S.G.; Shoseyov, O. Cloning, expression, characterization, and nucleophile identification of family 3, Aspergillus niger β-glucosidase. J. Biol. Chem. 2000, 275, 4973-4980.
7. Pontoh, J.; Low, N.H. Purification and characterization of beta-glucosidase from honey bees (Apis mellifera). Insect Biochem. Mol. Biol. 2002, 32, 679-690.
8. Park, J.D.; Rhee, D.K.; Lee, Y.H. Biological activities and chemistry of saponins from Panax ginseng C.A. Meyer. Phytochem. Rev. 2005, 4, 159-175.
9. 1 to 20(S)-ginsenoside Rg3 by Microbacterium sp. GS514. Phytochemistry 2008, 69, 218-224.
10. Zhao, X.; Gao, L.; Wang, J.; Bi, H.; Gao, J.; Du, X.; Zhou, Y.; Tai, G. A novel ginsenoside Rb1-hydrolyzing β-D-glucosidase from Cladosporium fulvum. Process Biochem. 2009, 44, 612-618.
11. Han, B.H.; Park, M.H.; Han, Y.N.; Woo, L.K.; Sankawa, U.; Yahara, S.; Tanaka, O. Degradation of ginseng under mild acidic condition. Planta Med. 1982, 44, 146-149.
12. Yang, L.; He, K.J.; Yang, Y. A preparation method of less polar individual ginsenosides and saponins by base hydrolysis. China Patent CN03134090.3, 30 March 2005.
13. Park, J.H. Sun ginseng-A new processed ginseng with fortified activity. Food Ind. Nutr. 2004, 9, 23-27.
14. Cheng, L.Q.; Na, J.R.; Kim, M.K.; Bang, M.H.; Yang, D.C. Microbial conversion of ginsenoside Rb1 to minor ginsenoside F2 and gypenoside XVII by Intrasporangium sp. GS603 isolated from soil. J. Micobiol. Biotechnol. 2007, 17, 1937-1943.
15. Schuster, E.; Dunn-Coleman, N.; Frisvad, J.C.; van Dijck, P.W.M. On the safety of Aspergillus niger-A review. Appl. Microbiol. Biotechnol. 2009, 59, 426-435.
16. Sung, C.K.; Lee, S.W.; Park, S.K.; Park, J.R.; Moon, I.L. Purification and characterization of β-glucosidase from Aspergillus niger SFN-416. Korean J. Appl. Microbiol. Biotechnol. 1997, 25, 44-50.
17. Rashid, M.H.; Siddiqui, K.S. Purification and characterization of a beta-glucosidase from Apsergillus niger. Folia Microbiol. 1997, 42, 544-550.
18. Watanabe, T.; Sato, T.; Yoshioka, S.; Koshijima, T.; Kuwahara, M. Purification and properties of Aspergillus niger β-glucosidase. Eur. J. Biochem. 1992, 209, 651-659.
19. Kaur, J.; Chadha, B.S.; Kumar, B.A.; Kaur, G.S.; Saini, H.S. Purification and characterization of β-glucosidase from Melanocarpus sp. MTCC 3922. Electron. J. Biotechnol. 2007, 10, 260-270.
20. Yan, T.R.; Lin, Y.H.; Lin, C.L. Purification and characterization of an extracellular β-glucosidase II with high hydrolysis and transglucosylation activities from Aspergillus niger. J. Agric. Food Chem. 1998, 46, 431-437.
21. Lee, J.K.; Choi, S.S.; Lee, H.K.; Han, K.J.; Han, E.J.; Suh, H.W. Effects of ginsenoside Rd and decursinol on the neurotoxic responses induced by kainic acid in mice. Planta Med. 2003, 69, 230-234.
22. Zeng, S.; Guan, Y.Y.; Liu, D.Y.; He, H.; Wang, W.; Qiu, Q.Y.; Wang, X.R.; Wang, Y.D. Synthesis of 12-epi-ginsenoside Rd and its effects on contractions of rat aortic rings. Chin. Pharmacol. Bull. 2003, 19, 282-286.
23. Tachikawa, E.; Kudo, K.; Hasegawa, H.; Kashimoto, T.; Sasaki, K.; Miyazaki, M.; Taira, H.; Lindstorm, J.M. In vitro inhibition of adrenal catecholamine secretion by steroidal metabolites of ginseng saponins. Biochem. Pharmacol. 2003, 66, 2213-2221.
24. Shinkai, K.; Akedo, H.; Mukai, M.; Imamura, F.; Isoai, A.; Kobayashi, M.; Kitagawa, I. Inhibition of in vitro tumor cell invasion by ginsenoside Rg3. Jpn. J. Cancer Res. 1996, 87, 357-362.
25. Lee, H.U.; Bae, E.A.; Han, M.J.; Kim, D.H. Hepatoprotective effect of 20(S)-ginsenoside Rg3 and its metabolite 20(S)-ginsenoside Rh2 on tert-butyl hydroperoxide-induced liver injury. Biol. Pharm. Bull. 2005, 28, 1992-1994.
26. Choi, I.Y.; Lee, J.H.; Kang, T.H.; An, H.J.; Kim, S.J.; Moon, P.D.; Kim, N.H.; Myung, N.Y.; Yand, D.C.; Kang, I.C. et al. Synergistic effects of KH-red ginseng/chlorella on the endurance capacity and immune enhancing in forced swimming tested mice. Food Sci. Biotech. 2009, 18, 1351-1357.
27. Kohchi, C.; Toh-E, A. Cloning of Candida pelliculosa beta-glucosidase gene and its expression in Saccharomyces cerevisiae. Mol. Gen. Genet. 1986, 203, 89-94.
28. Smith, P.K.; Krohn, R.I.; Hermanson, G.T.; Mallia, A.K.; Gartner, F.H.; Provenzano, M.D.; Fujimoto, E.K.; Goeke, N.M.; Olson, B.J.; Klenk, D.C. Measurement of protein using bicinchoninic acid. Anal. Biochem. 1985, 150, 76-85.
29. Laemmli, U.K. Cleavage of the structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685.
30. Luan, H.; Liu, X.; Qi, X.; Hu, Y.; Hao, D.; Cui, Y.; Yang, L. Purification and characterization of a novel stable ginsenoside Rb1-hydrolyzing β-D-glucosidase from China white jade snail. Process Biochem. 2006, 41, 1974-1980.
31. Chang, K.H.; Jee, H.S.; Lee, N.K.; Park, S.H.; Lee, N.W.; Paik, H.D. Optimization of the enzymatic production of 20(S)-ginsenoside Rg3 from white ginseng extract using response surface methodology. New Biotechnol. 2009, 26, 181-186.