Purification and characterization of β-glucosidase from honey bees (Apis mellifera)

Insect Biochemistry and Molecular Biology

Volumn 32, Issue 6, 2002, Pages 679-690

  Pontoh, J ^a   Low, N H ^a  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

Author keywords

Glucosidase; Characterization; Honey bee; Honey sac; Hypopharyngeal glands; MALDI TOF MS; Purification; Ventriculus

Indexed keywords

BETA GLUCOSIDASE; HYDROXYAPATITE;

ANIMAL; ANION EXCHANGE; ARTICLE; BEE; CATION EXCHANGE; ENZYME ANALYSIS; ENZYME GLYCOSYLATION; ENZYME KINETICS; ENZYME LOCALIZATION; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME SUBUNIT; ENZYMOLOGY; GEL PERMEATION CHROMATOGRAPHY; HONEYBEE; HYPOPHARYNX; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; METHODOLOGY; NONHUMAN; PEPTIDE MAPPING; PH; PH MEASUREMENT; POLYACRYLAMIDE GEL ELECTROPHORESIS; TEMPERATURE; TEMPERATURE SENSITIVITY; ISOLATION AND PURIFICATION;

APINAE; APIS; APIS MELLIFERA; APOIDEA;

ANIMALS; BEES; BETA-GLUCOSIDASE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYDROGEN-ION CONCENTRATION; PEPTIDE MAPPING; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TEMPERATURE;

EID: 0036015247     PISSN: 09651748     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0965-1748(01)00147-3     Document Type: Article

References (40)

1. Digestive enzymes and regional localization of proteolytic endopeptidases in the alimentary canal of the kola weevil, Sophrorhinus insperatus Faust (Coleoptera: Curculionidae)
2. Biochemistry of digestion
3. Beta-glucosidases in the rice weevil, Sitophiulus oryzae: Purification, properties and activity levels in wheat- and legume-feeding strains
4. Establishing optimum measurement conditions
5. Rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of proteindye binding
6. Purification by chromatography and properties of a β-glucosidase from the larvae of Phoracantha semipunctata
7. Purification and partial characterization of a cellobiase from the larvae of Rhagium inquisitor
8. Evolutionary considerations of the spatial organization of digestion in the luminescent predaceous larvae of Pyrearinus termitilluminans (Coleoptera: Elateridae)
9. β-glucosidases: Overview
10. Physical and kinetics properties of a plasma membrane bound β-D-glucosidase (cellobiose) from midgut cells of an insect (Rhynchosciara americana larvae)
11. Spatial organization of digestion, secretory mechanism and digestive enzyme properties in Pheropsophus aequinoctialis (Coleoptera: Carabidae)
12. The effect of dietary plant glycosidases on larval midgut β-glucosidases from Spodoptera frugiperda and Diatraea saccharalis
13. Substrate specificities of midgut β-glucosidases from insects of different orders
14. Mass spectrometric identification of proteins from silver-stained polyacrylamide gel: A method for the removal of silver ions to enhance sensitivity
15. Digestive enzymes and microorganisms in honey bees, Apis mellifera: Influence of streptomycin, age, season and pollen
16. Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases
17. A new enzyme, β-glucosidase, in honey
18. Carbohydrate analysis of Western Canadian honeys and their nectar sources to determine the origin of honey oligosaccharides
19. Midgut β-D-glucosidases from Abracris flavolineata (Orthoptera: Acrididae). Physical properties, substrate specificities and function
20. Purification and properties of a beta-glucosidase purified from midgut cells of Spodoptera frugiperda (lepidoptera) larvae
21. Relationship between gut cellobiase, lactase, aryl β-glucosidase, and aryl β-galactosidase activities of Locusta migratoria
22. Studies on glycosidases and glucanases in Thaumetopoea pityocampa larvae. II. Purification and some properties of a broad specificity β-D-glucosidase
23. Response surface cooptimization of reaction conditions in clinical chemical methods
24. Physical properties, substrate specificities and a probable mechanism for a β-D-glucosidase (cellobiase) from midgut cells of the cassava hornworm (Erinnyis ello)
25. Properties and compartmentalization of digestive carbohydrases and proteases in Scaptotrigona bipunctata (Apidae: Meliponinae) larvae
26. Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels
27. Enzyme markers and isolation of the microvillar and perimicrovillar membranes of Dysdercus peruvianus (Hemiptera: Pyrrhocoridae) midgut cells
28. The functions of the salivary glands of Apis mellifera
29. Invertase in the hypopharyngeal glands of the honeybee
30. The anatomy of the honey bee
31. Physiology and social physiology of the honey bee
32. Analysis and quantitation of the carbohydrates in honey using high performance liquid chromatography
33. Phylogenic considerations of insect digestion. Disaccharidases and the spatial organization of digestion in the Tenebrio molitor larvae
34. Origin, distribution, properties and functions of the major Rhodnius prolixus midgut hydrolases
35. Insect digestive enzymes: Properties, compartmentalization and function