Purification and Characterization of a β-Glucosidase from Aspergillus niger

Folia Microbiologica

Volumn 42, Issue 6, 1997, Pages 544-550

  Rashid, M H ^a   Siddiqui, K S ^a  

^a NATIONAL INSTITUTE FOR BIOTECHNOLOGY AND GENETIC ENGINEERING NIBGE   (Pakistan)

Author keywords

[No Author keywords available]

Indexed keywords

ASPERGILLUS NIGER;

AMMONIUM SULFATE; BETA GLUCOSIDASE; ENZYME INHIBITOR; FUNGAL PROTEIN; THERMOLYSIN;

ARTICLE; ASPERGILLUS NIGER; DRUG ANTAGONISM; ENZYMOLOGY; GEL CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR WEIGHT; PH; PRECIPITATION;

AMMONIUM SULFATE; ASPERGILLUS NIGER; BETA-GLUCOSIDASE; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; ENZYME INHIBITORS; FUNGAL PROTEINS; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR WEIGHT; PRECIPITATION; THERMOLYSIN;

EID: 0031305765     PISSN: 00155632     EISSN: None     Source Type: Journal    
DOI: 10.1007/bf02815462     Document Type: Article

References (20)

1. BENNETT J.W., KLICH MA.: Industrial enzymes from Aspergillus species, pp. 175-180 in Aspergillus: Biology and Industrial Applications (J.W. Bennett, MA. Klich, Eds). Butterworth-Heinemann, Boston 1992.
2. COUGHLAN M.P.: Staining techniques for the detection of the individual components of cellulolytic enzyme systems. Methods Enzymol. 160, 135-144 (1988).
3. DESHPANDE V., ERIKSSON K.E.: 1,4-β-Glucosidases of Sporotrichum pulverulentum. Methods Enzymol. 160, 415-424 (1988).
4. ESEN A.: β-Glucosidases, over review, pp. 1-14 in β-Glucosidases, Biochemistry and Molecular Biology (A. Esen, Ed.). American Chemical Society, Washington (DC) 1993.
5. ESEN A., GUNGOR G.: Stability and activity of plant and fungal β-glucosidases under denaturing conditions, pp. 214-239 in β-Glucosidases: Biochemistry and Molecular Biology (A. Esen, Ed.) American Chemical Society, Washington (DC) 1993.
6. GOLDENBERG D.P.: Analysis of protein conformation by gel electrophoresis, pp. 225-250 in Protein Structure: a Practicle Approach (T.E. Creighton, Ed.). IRL Press, Oxford (UK) 1989.
7. HIMMEL M.E., ADNEY W.S., FOX J.W., MITCHELL D.J., BAKER J.O.: Isolation and characterization of two forms of β-D-glucosidase from Aspergillus niger. Appl.Biochem.Biotechnol. 39/40, 213-225 (1993).
8. HOH Y.K., YEOH H.H., TAN T.K.: Isolation and characterization of β-glucosidase from Aspergillus nidulans mutant USDB 1183. World J.Microbiol.Biotechnol. 9, 555-558 (1993).
9. KVESITADZE G.I., SVANIDZE R.S., TSUPRUN V.L., NIZHARADZE D.N., CHIRGADZE L.T., BUACHIDZE T.S.: Quaternary structure and properties of β-glucosidase isolated from a thermophilic culture of Aspergillus wentii. Bioorg.Khim. 16, 881-888 (1990).
10. LAEMMLI U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685 (1970).
11. MCCLEARY B.V., HARRINGTON J.: Purification of β-glucosidase from Aspergillus niger. Methods Enzymol. 160, 575-583 (1988).
12. RASHID M.H., SIDDIQUI K.S.: The stability of extracellular β-glucosidase from Aspergillus niger is significantly enhanced by non-covalently attached polysaccharides. Folia Microbiol. 41, 341-346 (1996).
13. SADANA J.C., PATIL R.V., SHEWALE J.G.: β-D-Glucosidases from Sclerotium rolfsii. Methods Enzymol. 160, 424-431 (1988).
14. SANYAL A., KUNDU R.K., DUBE S., DUBE D.K.: Extracellular cellulytic enzyme system of Aspergillus japonicus: purification and characterization of an inducible extracellular β-glucosidase. Enzyme Microb.Technol. 10, 91-99 (1988).
15. SIDDIQUI K.S., RASHID M.H., RAJOKA M.I.: Kinetic analysis of the active site of an intracellular β-glucosidase from Cellulomonas biazotea. Folia Microbiol. 42, 53-58 (1997).
16. Sigma Technical Bulletin, no. MRK-137 (1986).
17. UNNO T., IDE K., YAZAKI T., TANAKA Y., NAKAKUKI T., OKADA G.: High recovery purification and some properties of a β-glucosidase from Aspergillus niger. Biosci.Biotech.Biochem. 57, 2172-2173 (1993).
18. VODJDANI G., DIZET P.L., PETEK F.: Purification et proprietes de deux (1-→4)-β-D-glucosidases d'Aspergillus roseus. Carbohydr.Res. 236, 267-279 (1992).
19. WOOD T.M., BHAT K.M.: Methods for measuring cellulase activities. Methods Enzymol. 160, 87-112 (1988).
20. XIMENES E.A., FELIX C.R., ULHOA C.J.: Production of cellulases by Aspergillus fumigatus and characterization of one β-glucosidase. Curr.Microbiol. 32, 119-123 (1996).