Evaluation of protective effects of trehalose on desiccation of epithelial cells in three dimensional reconstructed human corneal epithelium

Current Eye Research

Volumn 37, Issue 11, 2012, Pages 982-989

  Hovakimyan, Marine ^a   Ramoth, Theresa ^a   Löbler, Marian ^a   Schmitz, Klaus Peter ^a   Witt, Martin ^a   Guthoff, Rudolf ^a   Stachs, Oliver ^a  

^a UNIVERSITY OF ROSTOCK   (Germany)

Author keywords

3D culture system; Cornea; Dry eye; Epithelium; Trehalose

Indexed keywords

TREHALOSE;

ARTICLE; CELL PROTECTION; CELL VIABILITY; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; CORNEA EPITHELIUM; DESICCATION; DRY EYE; EPITHELIUM CELL; EX VIVO STUDY; HISTOLOGY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOFLUORESCENCE; IN VITRO STUDY; IN VIVO STUDY; KERATOPLASTY; MORPHOLOGY; OPTICAL COHERENCE TOMOGRAPHY; PRIORITY JOURNAL; THREE DIMENSIONAL IMAGING;

CELL COUNT; CELL SURVIVAL; DESICCATION; DRY EYE SYNDROMES; EPITHELIUM, CORNEAL; HUMANS; IMAGING, THREE-DIMENSIONAL; MICROSCOPY, CONFOCAL; OPHTHALMIC SOLUTIONS; TIGHT JUNCTIONS; TISSUE CULTURE TECHNIQUES; TOMOGRAPHY, OPTICAL COHERENCE; TREHALOSE;

EID: 84866909849     PISSN: 02713683     EISSN: 14602202     Source Type: Journal    
DOI: 10.3109/02713683.2012.700754     Document Type: Article

References (23)

1. Smith RE. The tear flm complex: pathogenesis and emerging therapies for dry eyes. Cornea 2005;24:1-7.
2. Gumus K, Cavanagh DH. The role of infammation and anti-infammation therapies in keratoconjunctivitis sicca. Clin Ophthalmol 2009;3:57-67.
3. Lemp MA, Foulks GN. The defnition and classifcation of dry eye disease: report of the Defnition and Classifcation Subcommittee of the International Dry Eye WorkShop. Ocular Surf 2007;5:75-92.
4. Crowe JH, Crowe LM. Preservation of mammalian cells-learning nature's tricks. Nat Biotechnol 2000;18:145-146.
5. Luyckx J, Baudouin C. Trehalose: an intriguing disaccharide with potential for medical application in ophthalmology. Clin Ophthalmol 2011;5:577-581.
6. Tang M, Waring AJ, Hong M. Trehalose-protected lipid membranes for determining membrane protein structure and insertion. J Magn Reson 2007;184:222-227.
7. Elbein AD, Pan YT, Pastuszak I et al. New insights on trehalose: a multifunctional molecule. Glycobiology 2003;13:17R-27R.
8. Carpenter JF, Martin B, Crowe LM et al. Stabilization of phosphofructokinase during air-drying with sugars and sugar/transition metal mixtures. Cryobiology 1987;24:455-464.
9. Crowe JH, Crowe LM, Carpenter JF et al. Stabilization of dry phospholipid bilayers and proteins by sugars. Biochem J 1987;242:1-10.
10. Matsuo T. Trehalose protects corneal epithelial cells from death by drying. Br J Ophthalmol 2001;85:610-612.
11. Chen W, Zhang X, Liu M et al. Trehalose protects against ocular surface disorders in experimental murine dry eye through suppression of apoptosis. Exp Eye Res 2009;89:311-318.
12. Doucet O, Lanvin M, Thillou C et al. Reconstituted human corneal epithelium: a new alternative to the Draize eye test for the assessment of the eye irritation potential of chemicals and cosmetic products. Toxicol In Vitro 2006;20:499-512.
13. Van Goethem F, Adriaens E, Alépée N et al. Prevalidation of a new in vitro reconstituted human cornea model to assess the eye irritating potential of chemicals. Toxicol In Vitro 2006;20:1-17.
14. Cotovio J, Grandidier MH, Lelièvre D et al. In vitro assessment of eye irritancy using the Reconstructed Human Corneal Epithelial SkinEthic HCE model: application to 435 substances from consumer products industry. Toxicol In Vitro 2010;24:523-537.
15. Nguyen DH, Beuerman RW, De Wever B et al. Alternative Toxicological Methods. (Eds.), USA: CRC Press, 2003; pp. 147-159.
16. Liang H, Pauly A, Riancho L et al. Toxicological evaluation of preservative-containing and preservative-free topical prostaglandin analogues on a three-dimensional-reconstituted corneal epithelium system. Br J Ophthalmol 2011;95:869-875.
17. Pauly A, Meloni M, Brignole-Baudouin F et al. Multiple endpoint analysis of the 3D-reconstituted corneal epithelium after treatment with benzalkonium chloride: early detection of toxic damage. Invest Ophthalmol Vis Sci 2009;50: 1644-1652.
18. Barabino S, Dana MR. Animal models of dry eye: a critical assessment of opportunities and limitations. Invest Ophthalmol Vis Sci 2004;45:1641-1646.
19. Lin Z, Liu X, Zhou T et al. A mouse dry eye model induced by topical administration of benzalkonium chloride. Mol Vis 2011;17:257-264.
20. Seo MJ, Kim JM, Lee MJ, et al. The therapeutic effect of DA-6034 on ocular infammation via suppression of MMP-9 and infammatory cytokines and activation of the MAPK signaling pathway in an experimental dry eye model. Curr Eye Res 2010;35:165-175.
21. Nakamura T, Sekiyama E, Takaoka M et al. The use of tre-halose-treated freeze-dried amniotic membrane for ocular surface reconstruction. Biomaterials 2008;29:3729-3737.
22. Teramoto N, Sachinvala ND, Shibata M. Trehalose and trehalose-based polymers for environmentally benign, biocompatible and bioactive materials. Molecules 2008;13: 1773-1816.
23. Meloni M, De Servi B, Marasco D et al. Molecular mechanism of ocular surface damage: application to an in vitro dry eye model on human corneal epithelium. Mol Vis 2011;17:113-126.