Trehalose-protected lipid membranes for determining membrane protein structure and insertion

Journal of Magnetic Resonance

Volumn 184, Issue 2, 2007, Pages 222-227

  Tang, Ming ^a   Waring, Alan J ^b   Hong, Mei ^a  

^a IOWA STATE UNIVERSITY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

13C 31P distances; Lipid bilayers; Membrane peptide structure; Solid state NMR; Trehalose

Indexed keywords

AMINO ACIDS; HYDROGEN BONDS; LIPIDS; PHASE TRANSITIONS; PROTEINS; TEMPERATURE MEASUREMENT;

LIPID BILAYERS; MEMBRANE PEPTIDE STRUCTURES; REHYDRATION; TREHALOSE;

BIOLOGICAL MEMBRANES;

DIPALMITOYLPHOSPHATIDYLCHOLINE; MEMBRANE PROTEIN; TREHALOSE; WATER;

ARTICLE; CHEMISTRY; LIPID BILAYER; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN CONFORMATION; ULTRASTRUCTURE;

1,2-DIPALMITOYLPHOSPHATIDYLCHOLINE; LIPID BILAYERS; MAGNETIC RESONANCE SPECTROSCOPY; MEMBRANE PROTEINS; PROTEIN CONFORMATION; TREHALOSE; WATER;

EID: 33846593768     PISSN: 10907807     EISSN: 10960856     Source Type: Journal    
DOI: 10.1016/j.jmr.2006.10.006     Document Type: Article

References (15)

1. Crowe J.H., Crowe L.M., and Chapman D. Preservation of membranes in anhydrobiotic organisms-the role of trehalose. Science 223 (1984) 701-703
2. Crowe J.H., Crowe L.M., Carpenter J.F., and Wistrom C.A. Stabilization of dry phospholipid-bilayers and proteins by sugars. Biochem. J. 242 (1987) 1-10
3. Anchordoquy T.J., Izutsu K.I., Randolph T.W., and Carpenter J.F. Maintenance of quaternary structure in the frozen state stabilizes lactate dehydrogenase during freeze-drying. Arch. Biochem. Biophys. 390 (2001) 35-41
4. Crowe J.H., Carpenter J.F., and Crowe L.M. The role of vitrification in anhydrobiosis. Annu. Rev. Physiol. 60 (1998) 73-103
5. Toke O., Maloy W.L., Kim S.J., Blazyk J., and Schaefer J. Secondary structure and lipid contact of a peptide antibiotic in phospholipid bilayers by REDOR. Biophys. J. 87 (2004) 662-674
6. Lee C.W., Das Gupta S.K., Mattai J., Shipley G.G., Abdel-Mageed O.H., Makriyannis A., and Griffin R.G. Characterization of the L lambda phase in trehalose-stabilized dry membranes by solid-state NMR and X-ray diffraction. Biochemistry 28 (1989) 5000-5009
7. Lee C.W., Waugh J.S., and Griffin R.G. Solid-state NMR study of trehalose/1,2-dipalmitoyl-sn-phosphatidylcholine interactions. Biochemistry 25 (1986) 3737-3742
8. Kohler S.J., and Klein M.P. Orientation and dynamics of phospholipid head groups in bilayers and membranes determined from P-31 nuclear magnetic-resonance chemical shielding tensors. Biochemistry 16 (1977) 519-526
9. Koster K.L., Webb M.S., Bryant G., and Lynch D.V. Interactions between soluble sugars and POPC (1-palmitoyl-2-oleoylphosphatidylcholine) during dehydration: vitrification of sugars alters the phase behavior of the phospholipid. Biochim. Biophys. Acta 1193 (1994) 143-150
10. Gullion T., and Schaefer J. Rotational echo double resonance NMR. J. Magn. Reson. 81 (1989) 196-200
11. Pearson R.H., and Pascher I. The molecular structure of lecithin dihydrate. Nature 281 (1979) 499-501
12. Heller H., Schaefer M., and Schulten K. Molecular-dynamics simulation of a bilayer of 200 lipids in the gel and in the liquid-crystal phases. J. Phys. Chem. 97 (1993) 8343-8360
13. Bellm L., Lehrer R.I., and Ganz T. Protegrins: new antibiotics of mammalian origin. Exp. Opin. Invest. Drugs 9 (2000) 1731-1742
14. Yamaguchi S., Hong T., Waring A., Lehrer R.I., and Hong M. Solid-state NMR investigations of peptide-lipid interaction and orientation of a beta-sheet antimicrobial peptide, protegrin. Biochemistry 41 (2002) 9852-9862
15. Sinha N., Schmidt-Rohr K., and Hong M. Compensation for pulse imperfections in rotational-echo double-resonance NMR by composite pulses and EXORCYCLE. J. Magn. Reson. 168 (2004) 358-365