Aspergillus-PCR in bronchoalveolar lavage for detection of invasive pulmonary aspergillosis in immunocompromised patients

BMC Infectious Diseases

Volumn 12, Issue , 2012, Pages

  Buess, Michael ^a   Cathomas, Gieri ^b   Halter, Jörg ^a   Junker, Lilian ^a   Grendelmeier, Peter ^a   Tamm, Michael ^a   Stolz, Daiana ^a  

^a UNIVERSITY HOSPITAL BASEL   (Switzerland)

^b Liestal Hospital   (Switzerland)

Author keywords

Bronchoscopy; Conventional nested PCR; Diagnosis; Invasive aspergillosis; Lung infection

Indexed keywords

ANTIFUNGAL AGENT; ANTIINFECTIVE AGENT; ANTIVIRUS AGENT; AZATHIOPRINE; CYCLOSPORIN; MYCOPHENOLIC ACID 2 MORPHOLINOETHYL ESTER; RAPAMYCIN; STEROID; TACROLIMUS;

ADULT; ARTICLE; ASPERGILLUS FLAVUS; ASPERGILLUS FUMIGATUS; ASPERGILLUS NIGER; ASPERGILLUS TERREUS; ASPERGILLUS TOMARRII; BRONCHOSCOPY; CLINICAL TRIAL; COMPUTER ASSISTED TOMOGRAPHY; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; EOSINOPHIL; EUROTIUM HERBARIORUM; FEMALE; FUNGAL DETECTION; GRAFT RECIPIENT; HEMATOLOGIC MALIGNANCY; HISTOPATHOLOGY; HUMAN; HUMAN TISSUE; IMMUNOCOMPROMISED PATIENT; LUNG ASPERGILLOSIS; LUNG INFECTION; LUNG LAVAGE; LYMPHOCYTE; MACROPHAGE; MAJOR CLINICAL STUDY; MALE; NEUTROPHIL; OBSERVATIONAL STUDY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STEM CELL TRANSPLANTATION; THORAX RADIOGRAPHY;

ADULT; AGED; AGED, 80 AND OVER; ASPERGILLUS FUMIGATUS; BRONCHOALVEOLAR LAVAGE FLUID; COHORT STUDIES; FEMALE; HUMANS; IMMUNOCOMPROMISED HOST; INVASIVE PULMONARY ASPERGILLOSIS; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; MYCOLOGY; POLYMERASE CHAIN REACTION; PROSPECTIVE STUDIES; SENSITIVITY AND SPECIFICITY; YOUNG ADULT;

EID: 84866780803     PISSN: None     EISSN: 14712334     Source Type: Journal    
DOI: 10.1186/1471-2334-12-237     Document Type: Article

References (40)

1. Herbrecht R, Denning DW, Patterson TF, Bennett JE, Greene RE, Oestmann JW, Kern WV, Marr KA, Ribaud P, Lortholary O, et al. Voriconazole versus amphotericin B for primary therapy of invasive aspergillosis. N Engl J Med 2002, 347(6):408-415. 10.1056/NEJMoa020191, 12167683.
2. Kim A, Nicolau DP, Kuti JL. Hospital costs and outcomes among intravenous antifungal therapies for patients with invasive aspergillosis in the United States. Mycoses 2011, 54(5):e301-12. Epub 2010 Jun 14, 10.1111/j.1439-0507.2010.01903.x, 20557463.
3. Reichenberger F, Habicht J, Matt P, Frei R, Soler M, Bolliger CT, Dalquen P, Gratwohl A, Tamm M. Diagnostic yield of bronchoscopy in histologically proven invasive pulmonary aspergillosis. Bone Marrow Transplant 1999, 24(11):1195-1199. 10.1038/sj.bmt.1702045, 10642808.
4. Weisser M, Rausch C, Droll A, Simcock M, Sendi P, Steffen I, Buitrago C, Sonnet S, Gratwohl A, Passweg J, et al. Galactomannan does not precede major signs on a pulmonary computerized tomographic scan suggestive of invasive aspergillosis in patients with hematological malignancies. Clin Infect Dis 2005, 41(8):1143-1149. 10.1086/444462, 16163633.
5. Danpornprasert P, Foongladda S, Tscheikuna J. Impact of bronchoalveolar lavage galactomannan on the outcome of patients at risk for invasive pulmonary aspergillosis. J Med Assoc Thai 2010, 93(Suppl 1):S86-93.
6. Clancy CJ, Jaber RA, Leather HL, Wingard JR, Staley B, Wheat LJ, Cline CL, Rand KH, Schain D, Baz M, et al. Bronchoalveolar lavage galactomannan in diagnosis of invasive pulmonary aspergillosis among solid-organ transplant recipients. J Clin Microbiol 2007, 45(6):1759-1765. 10.1128/JCM.00077-07, 1933052, 17428933.
7. Verweij PE, Latge JP, Rijs AJMM, Melchers WJG, Depauw BE, Hoogkampkorstanje JAA, Meis JFGM. Comparison of Antigen-Detection and Pcr Assay Using Bronchoalveolar Lavage Fluid for Diagnosing Invasive Pulmonary Aspergillosis in Patients Receiving Treatment for Hematological Malignancies. J Clin Microbiol 1995, 33(12):3150-3153. 228662, 8586691.
8. Pazos C, del Palacio A. Early diagnosis of invasive aspergillosis in neutropenic patients with bi-weekly serial screening of circulating galactomannan by Platelia Aspergillus. Rev Iberoam Micol 2003, 20(3):99-102.
9. Luong ML, Filion C, Labbe AC, Roy J, Pepin J, Cadrin-Tourigny J, Carignan S, Sheppard DC, Laverdiere M. Clinical utility and prognostic value of bronchoalveolar lavage galactomannan in patients with hematologic malignancies. Diagn Microbiol Infect Dis 2010, 68(2):132-139. 10.1016/j.diagmicrobio.2010.03.017, 20846585.
10. Reinwald M, Spiess B, Heinz WJ, Vehreschild JJ, Lass-Florl C, Kiehl M, Schultheis B, Krause SW, Wolf HH, Bertz H, et al. Diagnosing pulmonary aspergillosis in patients with hematological malignancies: a multicenter prospective evaluation of an Aspergillus PCR assay and a galactomannan ELISA in bronchoalveolar lavage samples. Eur J Haematol 2012, 89(2):120-7. Epub 2012 Jun 22, 10.1111/j.1600-0609.2012.01806.x, 22650156.
11. Moragues MD, Amutio E, Garcia-Ruiz JC, Ponton J. Usefulness of galactomannan detection in the diagnosis and follow-up of hematological patients with invasive aspergillosis. Rev Iberoam Micol 2003, 20(3):103-110.
12. Ascioglu S, Rex JH, de Pauw B, Bennett JE, Bille J, Crokaert F, Denning DW, Donnelly JP, Edwards JE, Erjavec Z, et al. Defining opportunistic invasive fungal infections in immunocompromised patients with cancer and hematopoietic stem cell transplants: an international consensus. Clin Infect Dis 2002, 34(1):7-14. 10.1086/323335, 11731939.
13. Hernandez Blasco L, Sanchez Hernandez IM, Villena Garrido V, de Miguel Poch E, Nunez Delgado M, Alfaro Abreu J. Safety of the transbronchial biopsy in outpatients. Chest 1991, 99(3):562-565. 10.1378/chest.99.3.562, 1995209.
14. Ahmad M, Livingston DR, Golish JA, Mehta AC, Wiedemann HP. The safety of outpatient transbronchial biopsy. Chest 1986, 90(3):403-405. 10.1378/chest.90.3.403, 3743154.
15. Ernst A, Eberhardt R, Wahidi M, Becker HD, Herth FJ. Effect of routine clopidogrel use on bleeding complications after transbronchial biopsy in humans. Chest 2006, 129(3):734-737. 10.1378/chest.129.3.734, 16537875.
16. Reichenberger F, Habicht J, Kaim A, Dalquen P, Bernet F, Schlapfer R, Stulz P, Perruchoud AP, Tichelli A, Gratwohl A, et al. Lung resection for invasive pulmonary aspergillosis in neutropenic patients with hematologic diseases. Am J Respir Crit Care Med 1998, 158(3):885-890.
17. Nebiker C, Lardinois D, Junker L, Gambazzi F, Matt P, Habicht J, Halter J, Heim D, Stern M, Buser A, et al. Lung resection in hematologic patients with pulmoary invasive fungal disease. Chest 2012, in press.
18. Khot PD, Ko DL, Hackman RC, Fredricks DN. Development and optimization of quantitative PCR for the diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid. BMC Infect Dis 2008, 8:73. 10.1186/1471-2334-8-73, 2440748, 18510764.
19. Tuon FF. A systematic literature review on the diagnosis of invasive aspergillosis using polymerase chain reaction (PCR) from bronchoalveolar lavage clinical samples. Rev Iberoam Micol 2007, 24(2):89-94. 10.1016/S1130-1406(07)70020-7, 17604424.
20. White PL, Perry MD, Loeffler J, Melchers W, Klingspor L, Bretagne S, McCulloch E, Cuenca-Estrella M, Finnstrom N, Donnelly JP, et al. Critical stages of extracting DNA from Aspergillus fumigatus in whole-blood specimens. J Clin Microbiol 2010, 48(10):3753-3755. 10.1128/JCM.01466-10, 2953093, 20720026.
21. Stolz D, Chhajed PN, Leuppi JD, Brutsche M, Pflimlin E, Tamm M. Cough suppression during flexible bronchoscopy using combined sedation with midazolam and hydrocodone: a randomised, double blind, placebo controlled trial. Thorax 2004, 59(9):773-776. 10.1136/thx.2003.019836, 1747138, 15333854.
22. Stolz D, Stulz A, Muller B, Gratwohl A, Tamm M. BAL neutrophils, serum procalcitonin, and C-reactive protein to predict bacterial infection in the immunocompromised host. Chest 2007, 132(2):504-514. 10.1378/chest.07-0175, 17573524.
23. De Pauw B, Walsh TJ, Donnelly JP, Stevens DA, Edwards JE, Calandra T, Pappas PG, Maertens J, Lortholary O, Kauffman CA, et al. Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group. Clin Infect Dis 2008, 46(12):1813-1821. 10.1086/588660, 2671227, 18462102.
24. Cathomas G, Stalder A, McGandy CE, Mihatsch MJ. Distribution of human herpesvirus 8 DNA in tumorous and nontumorous tissue of patients with acquired immunodeficiency syndrome with and without Kaposi's sarcoma. Modern pathology: an official journal of the United States and Canadian Academy of Pathology, Inc 1998, 11(5):415-420.
25. Pham AS, Tarrand JJ, May GS, Lee MS, Kontoyiannis DP, Han XY. Diagnosis of invasive mold infection by real-time quantitative PCR. Am J Clin Pathol 2003, 119(1):38-44. 10.1309/RQ05PP9NEG6DADXR, 12520695.
26. Zsikla V, Hailemariam S, Baumann M, Mund MT, Schaub N, Meier R, Cathomas G. Increased rate of Helicobacter pylori infection detected by PCR in biopsies with chronic gastritis. Am J Surg Pathol 2006, 30(2):242-248. 10.1097/01.pas.0000186397.18206.1b, 16434900.
27. Luong ML, Clancy CJ, Vadnerkar A, Kwak EJ, Silveira FP, Wissel MC, Grantham KJ, Shields RK, Crespo M, Pilewski J, et al. Comparison of an Aspergillus real-time polymerase chain reaction assay with galactomannan testing of bronchoalvelolar lavage fluid for the diagnosis of invasive pulmonary aspergillosis in lung transplant recipients. Clinical infectious diseases: an official publication of the Infectious Diseases Society of America 2011, 52(10):1218-1226.
28. Shahid M, Malik A, Bhargava R. Bronchogenic carcinoma and secondary aspergillosis-common yet unexplored: evaluation of the role of bronchoalveolar lavage-polymerase chain reaction and some nonvalidated serologic methods to establish early diagnosis. Cancer 2008, 113(3):547-558. 10.1002/cncr.23570, 18521930.
29. Raad I, Hanna H, Huaringa A, Sumoza D, Hachem R, Albitar M. Diagnosis of invasive pulmonary aspergillosis using polymerase chain reaction-based detection of aspergillus in BAL. Chest 2002, 121(4):1171-1176. 10.1378/chest.121.4.1171, 11948049.
30. Buchheidt D, Baust C, Skladny H, Ritter J, Suedhoff T, Baldus M, Seifarth W, Leib-Moesch C, Hehlmann R. Detection of Aspergillus species in blood and bronchoalveolar lavage samples from immunocompromised patients by means of 2-step polymerase chain reaction: clinical results. Clin Infect Dis 2001, 33(4):428-435. 10.1086/321887, 11462176.
31. Hayette MP, Vaira D, Susin F, Boland P, Christiaens G, Melin P, De Mol P. Detection of Aspergillus species DNA by PCR in bronchoalveolar lavage fluid. J Clin Microbiol 2001, 39(6):2338-2340. 10.1128/JCM.39.6.2338-2340.2001, 88140, 11376086.
32. Skladny H, Buchheidt D, Baust C, Krieg-Schneider F, Seifarth W, Leib-Mosch C, Hehlmann R. Specific detection of Aspergillus species in blood and bronchoalveolar lavage samples of immunocompromised patients by two-step PCR. J Clin Microbiol 1999, 37(12):3865-3871. 85831, 10565898.
33. Buchheidt D, Baust C, Skladny H, Baldus M, Brauninger S, Hehlmann R. Clinical evaluation of a polymerase chain reaction assay to detect Aspergillus species in bronchoalveolar lavage samples of neutropenic patients. Br J Haematol 2002, 116(4):803-811. 10.1046/j.0007-1048.2002.03337.x, 11886384.
34. Reinwald M, Hummel M, Kovalevskaya E, Spiess B, Heinz WJ, Vehreschild JJ, Schultheis B, Krause SW, Claus B, Suedhoff T, et al. Therapy with antifungals decreases the diagnostic performance of PCR for diagnosing invasive aspergillosis in bronchoalveolar lavage samples of patients with haematological malignancies. J Antimicrob Chemother 2012, 67(9):2260-7. Epub 2012 Jun 27, 10.1093/jac/dks208, 22740590.
35. Marr KA, Laverdiere M, Gugel A, Leisenring W. Antifungal therapy decreases sensitivity of the Aspergillus galactomannan enzyme immunoassay. Clin Infect Dis 2005, 40(12):1762-1769. 10.1086/429921, 15909264.
36. Rantakokko-Jalava K, Laaksonen S, Issakainen J, Vauras J, Nikoskelainen J, Viljanen MK, Salonen J. Semiquantitative detection by real-time PCR of Aspergillus fumigatus in bronchoalveolar lavage fluids and tissue biopsy specimens from patients with invasive aspergillosis. J Clin Microbiol 2003, 41(9):4304-4311. 10.1128/JCM.41.9.4304-4311.2003, 193834, 12958261.
37. Cathomas G. Molecular diagnostic in infectious disease pathology: an update. Ann Pathol 2009, 29(Spec No 1):S19-21.
38. Regamey N, Tamm M, Wernli M, Witschi A, Thiel G, Cathomas G, Erb P. Transmission of human herpesvirus 8 infection from renal-transplant donors to recipients. N Engl J Med 1998, 339(19):1358-1363. 10.1056/NEJM199811053391903, 9801396.
39. Hofman V, Dhouibi A, Butori C, Padovani B, Gari-Toussaint M, Garcia-Hermoso D, Baumann M, Venissac N, Cathomas G, Hofman P. Usefulness of molecular biology performed with formaldehyde-fixed paraffin embedded tissue for the diagnosis of combined pulmonary invasive mucormycosis and aspergillosis in an immunocompromised patient. Diagn Pathol 2010, 5:1. 10.1186/1746-1596-5-1, 2823679, 20205795.
40. Tamm M, Traenkle P, Grilli B, Soler M, Bolliger CT, Dalquen P, Cathomas G. Pulmonary cytomegalovirus infection in immunocompromised patients. Chest 2001, 119(3):838-843. 10.1378/chest.119.3.838, 11243966.