Specific detection of Aspergillus species in blood and bronchoalveolar lavage samples of immunocompromised patients by two-step PCR

Journal of Clinical Microbiology

Volumn 37, Issue 12, 1999, Pages 3865-3871

  Skladny, Heyko ^a   Buchheidt, Dieter ^a,c   Baust, Corinna ^a   Krieg Schneider, Frank ^a,d   Seifarth, Wolfgang ^a   Leib Mösch, Christine ^a,b   Hehlmann, Rüdiger ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b INSTITUTE OF EPIDEMIOLOGY   (Germany)

^c UNIVERSITY OF HEIDELBERG   (Germany)

^d QIAGEN GMBH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACCURACY; ARTICLE; ASPERGILLOSIS; ASPERGILLUS; BLOOD ANALYSIS; CONTROLLED STUDY; EARLY DIAGNOSIS; HIGH RISK PATIENT; HUMAN; IMMUNE DEFICIENCY; LUNG LAVAGE; MAJOR CLINICAL STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ASPERGILLUS; BACTERIA (MICROORGANISMS); CANDIDA;

EID: 0032728086     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.37.12.3865-3871.1999     Document Type: Article

References (54)

1. Anaissie, E. J., G. P. Bodey, and M. G. Rinaldi. 1989. Emerging fungal pathogens. Eur. J. Clin. Microbiol. Infect. Dis. 8:323-330.
2. Aquino, S. L., S. T. Kee, M. L. Warnock, and G. Gamsu. 1994. Pulmonary aspergillosis: imaging findings with pathologic correlation. Am. J. Roentgenol. 163:811-815.
3. Arning, M., and C. Aul. 1994. Prophylaxis against mycosis in neutropenic patients. Mycoses 37(Suppl. 2):70-76.
4. Aufavre-Brown, A., J. Cohen, and D. W. Holden. 1992. Use of randomly amplified polymorphic DNA markers to distinguish isolates of Aspergillus fumigatus. J. Clin. Microbiol. 30:2991-2993.
5. Birch, M., N. Nolard, G. S. Shankland, and D. W. Denning. 1995. DNA typing of epidemiologically related isolates of Aspergillus fumigatus. Epidemiol. Infect. 114:161-168.
6. Blum, U., M. Windfuhr, C. Buitrago-Tellez, G. Sigmund, E. W. Herbst, and M. Langer. 1994. Invasive pulmonary aspergillosis. MRI, CT and plain radiographic findings and their contribution for early diagnosis. Chest 106: 1156-1161.
7. Bodey, G. P., B. Bueltmann, W. Duguid, D. Gibbs, H. Hanak, M. Hotchi, G. Mall, P. Martino, F. Meunier, S. Milllken, S. Naoe, M. Okudaira, D. Scevola, and J. van't Wout. 1992. Fungal infections in cancer patients: an international autopsy survey. Eur. J. Clin. Microbiol. Infect. Dis. 11:99-109.
8. Bretagne, S., J.-M. Costa, A. Marmorat-Khuong, F. Poron, C. Cordonnier, M. Vidaud, and J. Fleury-Feith. 1995. Detection of Aspergillus species DNA in bronchoalveolar lavage samples by competitive PCR. J. Clin. Microbiol. 33:1164-1168.
9. Burg, J. L., M. Grover, P. Pouletty, and J. C. Boothroyd. 1989. Direct and sensitive detection of a pathogenic protozoon, Toxoplasma gondii, by polymerase chain reaction. J. Clin. Microbiol. 27:1787-1792.
10. Cafferkey, M. T. 1994. Chemoprophylaxis of invasive pulmonary aspergillosis. J. Antimicrob. Chemother. 33:917-924.
11. Caillot, D., O. Casasnovas, A. Bernard, J. F. Couaillier, C. Durand, B. Cuisenier, E. Solary, F. Piard, T. Petrella, A. Bonnin, G. Couillault, M. Dumas, and H. Guy. 1997. Improved management of invasive pulmonary aspergillosis in neutropenic patients using early thoracic computed tomography scan and surgery. J. Clin. Oncol. 15:139-147.
12. Chandrasekar, P. H., A. Weinmann, and C. Shearer. 1995. Autopsy-identified infections among bone marrow transplant recipients: a clinico-pathologic study of 56 patients. Bone Marrow Transplant. 16:675-681.
13. Denning, D. W. 1994. Treatment of invasive aspergillosis. J. Infect. 28:25-33.
14. Deutsche Gesellschaft fur Pneumologie. 1994. Empfehlungen zur diagnostischen bronchoalveolären Lavage. Pneumologie 48:311-323.
15. Duthie, R., and D. W. Denning. 1995. Aspergillus fungemia: report of two cases and review. Clin. Infect. Dis. 20:598-605.
16. Einsele, H., H. Steidle, A. Vallbracht, J. G. Saal, G. Ehninger, and C. A. Müller. 1991. Early occurrence of human cytomegalovirus infection after bone marrow transplantation as demonstrated by the polymerase chain reaction technique. Blood 77:1104-1110.
17. Einsele, H., H. Hebart, G. Roller, J. Löffler, I. Rothenhöfer, C. A. Müller, R. A. Bowden, J.-A. van Burik, D. Engelhard, L. Kanz, and U. Schumacher. 1997. Detection and identification of fungal pathogens in blood using molecular probes. J. Clin. Microbiol. 35:1353-1360.
18. Girardin, H., J. Sarfati, F. Traore, J. Dupouy-Camet, F. Derouin, and J. P. Latge. 1994. Molecular epidemiology of nosocomial invasive aspergillosis. J. Clin. Microbiol. 32:684-690.
19. Glass, N. L., and G. C. Donaldson. 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl. Environ. Microbiol. 61:1323-1330.
20. Groll, A. H., P. M. Shah, C. Mentzel, M. Schneider, G. Just-Nübling, and K. Huebner. 1996. Trends in the postmortem epidemiology of invasive fungal infections at a university hospital. J. Infect. 33:23-32.
21. Guiot, H. F. L., W. E. Fibbe, and J. W. van't Wout. 1994. Risk factors for fungal infection in patients with malignant hematologic disorders: implications for empirical therapy and prophylaxis. Clin. Infect. Dis. 18:525-532.
22. Haynes, K., and T. R. Rogers. 1994. Retrospective evaluation of a latex agglutination test for diagnosis of invasive aspergillosis in immunocompromised patients. Eur. J. Clin. Microbiol. Infect. Dis. 13:670-674.
23. Hearn, V. M., C. Pinel, S. Blachier, P. Ambroise-Thomas, and R. Grillot. 1995. Specific antibody detection in invasive aspergillosis by analytical isoelectrofocusing and immunoblotting methods. J. Clin. Microbiol. 33:982-986.
24. Hehlmann, R., H. Skladny, and D. Buchheidt. 16 February 1999. Verfahren zum Nachweis von Aspergillus-Nukleinsäuren in einer Körperprobe, Nukleinsäure-Sonden und Diagnosekits zur Durchführung des Verfahrens. Patent no. 198 06 274.5. German Patent Office.
25. Huppertz, H.-I., H. Schmidt, and H. Karch. 1993. Detection of Borrelia burgdorferi by nested polymerase chain reaction in cerebrospinal fluid and urine of children with neuroborreliosis. Eur. J. Pediatr. 152:414-418.
26. Kappe, R., A. Schulze-Berge, and H. G. Sonntag. 1996. Evaluation of eight antibody tests and one antigen test for the diagnosis of invasive aspergillosis. Mycoses 39:13-23.
27. Kuhlmann, J. E., E. K. Fishman, P. A. Burch, J. E. Karp, E. A. Zerhouni, and S. S. Siegelman. 1987. Invasive pulmonary aspergillosis in acute leukemia: the contribution of CT to early diagnosis and aggressive management. Chest 92:95-99.
28. Logan, P. M., S. L. Primack, R. R. Miller, and N. L. Muller. 1994. Invasive aspergillosis of the airways: radiographic, CT and pathologic findings. Radiology 193:383-388.
29. Makimura, K., S. Y. Murayama, and H. Yamaguchi. 1994. Specific detection of Aspergillus and Penicillium species from respiratory specimens by PCR. Jpn. J. Med. Sci. Biol. 47:141-156.
30. Makimura, K., S. Y. Murayama, and H. Yamaguchi. 1994. Detection of a wide range of medically important fungi by the polymerase chain reaction. J. Med. Microbiol. 40:358-364.
31. Melchers, W. J., P. E. Verweij, P. van den Hurk, A. ran Belkum, B. E. De Pauw, J. A. Hoogkamp-Korstanje, and J. F. Meis. 1994. General primer-mediated PCR for detection of Aspergillus species. J. Clin. Microbiol. 32: 1710-1717.
32. Morrison, V. A., R. J. Haake, and D. J. Weisdorf. 1993. The spectrum of non-Candida fungal infections following bone marrow transplantation. Medicine 72:78-89.
33. Obayashi, T., M. Yoshida, T. Mori, H. Goto, A. Yasuoka, H. Iwasaki, H. Teshima, S. Kohno, A. Horiuchi, and A. Ito. 1994. Plasma(1-3)-beta-D-glucan measurement in diagnosis of invasive deep mycosis and fungal febrile episodes. Lancet 345:17-20.
34. Ou, C. Y., S. Kwok, W. Mitchell, D. H. Mack, J. J. Sninsky, J. W. Krebs, P. Feorino, G. Warfield, and G. Schochetman. 1988. DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells. Science 239:295-297.
35. Patterson, T. F., P. Miniter, J. E. Patterson, J. M. Rappaport, and V. T. Andriole. 1995. Aspergillus antigen detection in the diagnosis on invasive aspergillosis. J. Infect. Dis. 171:1553-1558.
36. Pfaffenbach, B., K. Donhuijsen, J. Pahnke, R. Bug, R. J. Adamek, M. Wegener, and D. Ricken. 1994. Systemische Pilzinfektionen bei hämatologischen Neoplasien. Eine Autopsiestudie an 1053 Patienten. Med. Klin. 89: 299-304.
37. Rinaldi, M. G. 1991. Problems in the diagnosis of invasive fungal diseases. Rev. Infect. Dis. 13:493-498.
38. Robinson, L. A., E. C. Reed, T. A. Galbraith, A. Alonso, A. L. Moulton, and W. H. Fleming. 1995. Pulmonary resection for invasive Aspergillus infections in immunocompromised patients. J. Thorac. Cardiovasc. Surg. 109:1182-1196.
39. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
40. Saugier-Veber, P., A. Devergie, A. Sulahian, et al. 1993. Epidemiology and diagnosis of invasive pulmonary aspergillosis in bone marrow transplant patients. Results of a 5 year retrospective study. Bone Marrow Transplant. 12:121-124.
41. Shibata, D., J. W. Martin, M. D. Appleman, D. M. Causey, J. M. Leedom, and N. Arnheim. 1988. Detection of cytomegalavirus DNA in peripheral blood of patients infected with human immunodeficiency virus. J. Infect. Dis. 158:1185-1192.
42. Spreadbury, C., D. Holden, A. Aufavre-Brown, B. Bainbridge, and J. Cohen. 1993. Detection of Aspergillus fumigatus by polymerase chain reaction. J. Clin. Microbiol. 31:615-621.
43. Stynen, D., A. Coris, J. Sarfati, and J. P. Latge. 1995. A new sensitive sandwich enzyme-linked immunosorbent assay to detect galactofuran in patients with invasive aspergillosis. J. Clin. Microbiol. 33:497-500.
44. Swanink, C. M. A., J. F. G. M. Meis, A. J. M. M. Rijs, J. P. Donnelly, and P. E. Verweij. 1997. Specificity of a sandwich enzyme linked immunosorbent assay for detecting Aspergillus galactomannan. J. Clin. Microbiol. 35:257-260.
45. Tang, C. M., D. Holden, A. Aufauvre-Brown, and J. Cohen. 1993. Detection of Aspergillus species by the polymerase chain reaction and its evaluation in bronchoalveolar lavage fluid. Am. Rev. Respir. Dis. 148:1313-1317.
46. van Burik, J.-A., D. Myerson, R. W. Schreckhise, and R. A. Bowden. 1998. Panfungal PCR assay for detection of fungal infection in human blood specimens. J. Clin. Microbiol. 36:1169-1175.
47. Verweg, P. E., A. J. Rijs, B. E. De Pauw, A. M. Horrevorts, J. A. Hoogkamp-Korstanje, and J. F. Meis. 1995. Clinical evaluation and reproducibility of the Pastorex Aspergillus antigen latex test for diagnosing invasive aspergillosis. J. Clin. Pathol. 48:474-476.
48. Verweij, P. E., J. P. Latge, A. J. Rijs, W. J. Melchers, B. E. de Pauw, J. A. Hoogkamp-Korstanje, and J. F. Meis. 1995. Comparison of antigen detection and PCR assay using bronchoalveolar lavage fluid for diagnosing invasive pulmonary aspergillosis in patients receiving treatment for hematological malignancies. J. Clin. Microbiol. 33:3150-3153.
49. von Eiff, M., M. Zuhlsdorf, N. Roos, M. Hesse, R. Schulten, and J. van de Loo. 1995. Pulmonary fungal infections in patients with hematological malignancies: diagnostic approaches. Ann. Hematol. 70:135-141.
50. Walsh, T. J., K. Garrett, E. Feuerstein, M. Girton, M. Allende, J. Bacher, A. Francesconi, R. Schaufele, and P. A. Pizzo. 1995. Therapeutic monitoring of experimental invasive pulmonary aspergillosis by ultrafast computerized tomography: a novel, noninvasive method for measuring responses to antifungal therapy. Antimicrob. Agents Chemother. 39:1065-1069.
51. Walsh, T. J., J. Lee, J. Lecciones, M. Rubin, K. Butler, P. Francis, M. Weinberger, E. Roilides, D. Marshall, J. Gress, and P. A. Pizzo. 1991. Empiric therapy with amphotericin B in febrile granulocytopenic patients. Rev. Infect. Dis. 13:496-503.
52. Walsh, T. J., A. Francesconi, M. Kasai, and S. J. Chanock. 1995. PCR and single-strand conformational polymorphism for recognition of medically important opportunistic fungi. J. Clin. Microbiol. 33:3216-3220.
53. Yamakami, Y., A. Hashimoto, I. Tokimatsu, and M. Nasu. 1996. PCR detection of DNA specific for Aspergillus species in serum of patients with invasive aspergillosis. J. Clin. Microbiol. 34:2464-2468.
54. Yu, V. L., R. R. Muder, and A. Poorsattar. 1986. Significance of isolation of Aspergillus from the respiratory tract in diagnosis of invasive pulmonary aspergillosis. Am. J. Med. 81:249-254.