Critical stages of extracting DNA from Aspergillus fumigatus in whole-blood specimens

Journal of Clinical Microbiology

Volumn 48, Issue 10, 2010, Pages 3753-3755

  White, P Lewis ^a   Perry, Michael D ^a   Loeffler, Juergen ^b   Melchers, Willem ^c   Klingspor, Lena ^d   Bretagne, Stephane ^e   McCulloch, Elaine ^f   Cuenca Estrella, Manuel ^g   Finnstrom, Niklas ^h   Donnelly, J Peter ^c   Barnes, Rosemary A ⁱ  

^a UNIVERSITY HOSPITAL OF WALES   (United Kingdom)

^b UNIVERSITY OF WÜRZBURG   (Germany)

^c RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

^d KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^e HENRI MONDOR HOSPITAL   (France)

^f ROYAL HOSPITAL FOR SICK CHILDREN   (United Kingdom)

^g National Microbiological Institute   (Spain)

^h Cepheid AB   (Sweden)

ⁱ CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ARTICLE; ASPERGILLUS FUMIGATUS; BLOOD; CONTROLLED STUDY; CYTOLYSIS; DNA EXTRACTION; DNA PURIFICATION; INTERNAL CONSISTENCY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY ANALYSIS;

ASPERGILLOSIS; ASPERGILLUS FUMIGATUS; BLOOD; DNA, FUNGAL; HUMANS; MYCOLOGY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ASPERGILLUS; ASPERGILLUS FUMIGATUS;

EID: 77957765288     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01466-10     Document Type: Article

References (6)

1. De Pauw, B., T. J. Walsh, J. P. Donnelly, D. A. Stevens, J. E. Edwards, T. Calandra, P. G. Pappas, J. Maertens, O. Lortholary, C. A. Kauffman, D. W. Denning, T. F. Patterson, G. Maschmeyer, J. Bille, W. E. Dismukes, R. Herbrecht, W. W. Hope, C. C. Kibbler, B. J. Kullberg, K. A. Marr, P. Muñoz, F. C. Odds, J. R. Perfect, A. Restrepo, M. Ruhnke, B. H. Segal, J. D. Sobel, T. C. Sorrell, C. Viscoli, J. R. Wingard, T. Zaoutis, J. E. Bennett, European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group, and National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group. 2008. Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group. Clin. Infect. Dis. 46:1813-1821.
2. Herrera, M. L., A. C. Vallor, J. A. Gelfond, T. F. Patterson, and B. L. Wickes. 2009. Strain-dependent variation in 18S ribosomal DNA copy numbers in Aspergillus fumigatus. J. Clin. Microbiol. 47:1325-1332.
3. Loeffler, J., N. Henke, H. Hebart, D. Schmidt, L. Hagmeyer, U. Schumacher, and H. Einsele. 2000. Quantification of fungal DNA by using fluorescence resonance energy transfer and the light cycler system. J. Clin. Microbiol. 38:586-590.
4. Millon, L., R. Piarroux, E. Deconinck, C. E. Bulabois, F. Grenouillet, P. Rohrlich, J. M. Costa, and S. Bretagne. 2005. Use of real-time PCR to process the first galactomannan-positive serum sample in diagnosing invasive aspergillosis. J. Clin. Microbiol. 43:5097-5101.
5. White, P. L., S. Bretagne, L. Klingspor, W. J. G. Melchers, E. McCulloch, B. Schulz, N. Finnstrom, C. Mengoli, R. A. Barnes, J. P. Donnelly, and J. Loeffler on behalf of the European Aspergillus PCR Initiative. 2010. Aspergillus PCR: one step closer towards standardization. J. Clin. Microbiol. 48:1231-1240.
6. White, P. L., C. J. Linton, M. D. Perry, E. M. Johnson, and R. A. Barnes. 2006. The evolution and evaluation of a whole blood polymerase chain reaction assay for the detection of invasive aspergillosis in hematology patients in a routine clinical setting. Clin. Infect. Dis. 42:479-486.