Therapy with antifungals decreases the diagnostic performance of PCR for diagnosing invasive aspergillosis in bronchoalveolar lavage samples of patients with haematological malignancies

Journal of Antimicrobial Chemotherapy

Volumn 67, Issue 9, 2012, Pages 2260-2267

  Reinwald, Mark ^a   Hummel, Margit ^a   Kovalevskaya, Elena ^a   Spiess, Birgit ^a   Heinz, Werner J ^b   Vehreschild, Jörg Janne ^c   Schultheis, Beate ^d   Krause, Stefan W ^e   Claus, Bernd ^f   Suedhoff, Thomas ^g   Schwerdtfeger, Rainer ^h   Reuter, Stefan ⁱ   Kiehl, Michael G ^j   Hofmann, Wolf Karsten ^a   Buchheidt, Dieter ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b UNIVERSITY HOSPITAL WÜRZBURG   (Germany)

^c UNIVERSITY HOSPITAL OF COLOGNE   (Germany)

^d RUHR UNIVERSITY BOCHUM   (Germany)

^e UNIVERSITY HOSPITAL ERLANGEN   (Germany)

^f Ludwigshafen General Hospital   (Germany)

^g Department of Internal Medicine   (Germany)

^h Bone Marrow Transplantation Center Wiesbaden   (Germany)

ⁱ UNIVERSITY HOSPITAL ULM   (Germany)

^j Frankfurt/Oder General Hospital   (Germany)

more..

Author keywords

Aspergillus PCR; BAL; IA

Indexed keywords

AMPHOTERICIN B; ANIDULAFUNGIN; ANTIFUNGAL AGENT; CASPOFUNGIN; ITRACONAZOLE; POSACONAZOLE; VORICONAZOLE;

ADOLESCENT; ADULT; AGED; ANTIMICROBIAL THERAPY; ARTICLE; ASPERGILLUS; CANCER PATIENT; CHEMOPROPHYLAXIS; CHILD; COMBINATION CHEMOTHERAPY; DIAGNOSTIC ACCURACY; DRUG EFFECT; FEMALE; HEMATOLOGIC MALIGNANCY; HIGH RISK PATIENT; HUMAN; INVASIVE ASPERGILLOSIS; LUNG LAVAGE; MAJOR CLINICAL STUDY; MALE; POLYMERASE CHAIN REACTION; PRESCHOOL CHILD; RETROSPECTIVE STUDY; SCHOOL CHILD; SENSITIVITY AND SPECIFICITY;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; ANTIFUNGAL AGENTS; ASPERGILLOSIS; ASPERGILLUS; BRONCHOALVEOLAR LAVAGE FLUID; CHEMOPREVENTION; CHILD; CHILD, PRESCHOOL; FEMALE; HEMATOLOGIC NEOPLASMS; HUMANS; IMMUNOCOMPROMISED HOST; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; MYCOLOGY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; YOUNG ADULT;

EID: 84865349023     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dks208     Document Type: Article

References (36)

1. De Pauw B, Walsh TJ, Donnelly JP et al. Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group. Clin Infect Dis 2008; 46: 1813-21.
2. Pappas PG, Alexander BD, Andes DR et al. Invasive fungal infections among organ transplant recipients: results of the Transplant-Associated Infection Surveillance Network (TRANSNET). Clin Infect Dis 2010; 50: 1101-11.
3. Kim A, Nicolau DP, Kuti JL. Hospital costs and outcomes among intravenous antifungal therapies for patients with invasive aspergillosis in the United States. Mycoses 2011; 54: e301-12.
4. Chamilos G, Luna M, Lewis RE et al. Invasive fungal infections in patients with hematologic malignancies in a tertiary care cancer center: an autopsy study over a 15-year period (1989-2003). Haematologica 2006; 91: 986-9.
5. White PL, Bretagne S, Klingspor L et al. Aspergillus PCR: one step closer to standardization. J Clin Microbiol 2010; 48: 1231-40.
6. Buchheidt D, Baust C, Skladny H et al. Detection of Aspergillus species in blood and bronchoalveolar lavage samples from immunocompromised patients by means of 2-step polymerase chain reaction: clinical results. Clin Infect Dis 2001; 33: 428-35.
7. Cornely OA, Maertens J, Winston DJ et al. Posaconazole vs. fluconazole or itraconazole prophylaxis in patients with neutropenia. N Engl J Med 2007; 356: 348-59.
8. Lass-Flörl C, Gunsilius E, Gastl G et al. Diagnosing invasive aspergillosis during antifungal therapy by PCR analysis of blood samples. J Clin Microbiol 2004; 42: 4154-7.
9. Musher B, Fredricks D, Leisenring W et al. Aspergillus galactomannan enzyme immunoassay and quantitative PCR for diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid. J Clin Microbiol 2004; 42: 5517-22.
10. Hummel M, Spiess B, Cornely OA et al. Aspergillus PCR testing: results from a prospective PCR study within the AmBiLoad trial. Eur J Haematol 2010; 85: 164-9.
11. McCulloch E, Ramage G, Rajendran R et al. Antifungal treatment affects the laboratory diagnosis of invasive aspergillosis. J Clin Pathol 2012; 65: 83-6.
12. Francesconi A, Kasai M, Petraitiene R et al. Characterization and comparison of galactomannan enzyme immunoassay and quantitative real-time PCR assay for detection of Aspergillus fumigatus in bronchoalveolar lavage fluid from experimental invasive pulmonary aspergillosis. J Clin Microbiol 2006; 44: 2475-80.
13. Skladny H, Buchheidt D, Baust C et al. Specific detection of Aspergillus species in blood and bronchoalveolar lavage samples of immunocompromised patients by two-step PCR. J Clin Microbiol 1999; 37: 3865-71.
14. Maschmeyer G, Beinert T, Buchheidt D et al. Diagnosis and antimicrobial therapy of lung infiltrates in febrile neutropenic patients: guidelines of the Infectious Diseases Working Party of the German Society of Haematology and Oncology. Eur J Cancer 2009; 45: 2462-72.
15. [Recommendations for diagnostic bronchoalveolar lavage. German Society of Pneumology]. Pneumologie 1994; 48 Suppl 1: 311-23.
16. Maschmeyer G, Beinert T, Buchheidt D et al. [Diagnosis and therapy of lung infiltrates in febrile neutropenic patients. Standard recommendations of the Work Group of Infections in Hematology and Oncology of the German Association of Hematology and Oncology]. Dtsch Med Wochenschr 1999; 124 Suppl 1: S18-23.
17. Maschmeyer G, Beinert T, Buchheidt D et al. Diagnosis and antimicrobial therapy of pulmonary infiltrates in febrile neutropenic patients-guidelines of the Infectious Diseases Working Party (AGIHO) of the German Society of Hematology and Oncology (DGHO). Ann Hematol 2003; 82 Suppl 2: S118-26.
18. Tuon FF. A systematic literature review on the diagnosis of invasive aspergillosis using polymerase chain reaction (PCR) from bronchoalveolar lavage clinical samples. Rev Iberoam Micol 2007; 24: 89-94.
19. Ascioglu S, Rex JH, De Pauw B et al. Defining opportunistic invasive fungal infections in immunocompromised patients with cancer and hematopoietic stem cell transplants: an international consensus. Clin Infect Dis 2002; 34: 7-14.
20. Verweij PE, Latge JP, Rijs AJ et al. Comparison of antigen detection and PCR assay using bronchoalveolar lavage fluid for diagnosing invasive pulmonary aspergillosis in patients receiving treatment for hematological malignancies. J Clin Microbiol 1995; 33: 3150-3.
21. Buchheidt D, Baust C, Skladny H et al. Clinical evaluation of a polymerase chain reaction assay to detect Aspergillus species in bronchoalveolar lavage samples of neutropenic patients. Br J Haematol 2002; 116: 803-11.
22. Khot PD, Ko DL, Hackman RC et al. Development and optimization of quantitative PCR for the diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid. BMC Infect Dis 2008; 8: 73.
23. Frealle E, Decrucq K, Botterel F et al. Diagnosis of invasive aspergillosis using bronchoalveolar lavage in haematology patients: influence of bronchoalveolar lavage human DNA content on real-time PCR performance. Eur J Clin Microbiol Infect Dis 2009; 28: 223-32.
24. Herbrecht R, Denning DW, Patterson TF et al. Voriconazole versus amphotericin B for primary therapy of invasive aspergillosis. N Engl J Med 2002; 347: 408-15.
25. Maertens J, Raad I, Petrikkos G et al. Efficacy and safety of caspofungin for treatment of invasive aspergillosis in patients refractory to or intolerant of conventional antifungal therapy. Clin Infect Dis 2004; 39: 1563-71.
26. Walsh TJ, Raad I, Patterson TF et al. Treatment of invasive aspergillosis with posaconazole in patients who are refractory to or intolerant of conventional therapy: an externally controlled trial. Clin Infect Dis 2007; 44: 2-12.
27. Schiel X, Link H, Maschmeyer G et al. A prospective, randomized multicenter trial of the empirical addition of antifungal therapy for febrile neutropenic cancer patients: results of the Paul Ehrlich Society for Chemotherapy (PEG) Multicenter Trial II. Infection 2006; 34: 118-26.
28. Kami M, Machida U, Okuzumi K et al. Effect of fluconazole prophylaxis on fungal blood cultures: an autopsy-based study involving 720 patients with haematological malignancy. Br J Haematol 2002; 117: 40-6.
29. Marr KA, Laverdiere M, Gugel A et al. Antifungal therapy decreases sensitivity of the Aspergillus galactomannan enzyme immunoassay. Clin Infect Dis 2005; 40: 1762-9.
30. Guo YL, Chen YQ, Wang K et al. Accuracy of BAL galactomannan in diagnosing invasive aspergillosis: a bivariate metaanalysis and systematic review. Chest 2010; 138: 817-24.
31. Koo S, Bryar JM, Page JH et al. Diagnostic performance of the (1→3)-β-D-glucan assay for invasive fungal disease. Clin Infect Dis 2009; 49: 1650-9.
32. Bretagne S, Costa JM, Bart-Delabesse E et al. Comparison of serum galactomannan antigen detection and competitive polymerase chain reaction for diagnosing invasive aspergillosis. Clin Infect Dis 1998; 26: 1407-12.
33. des Champs-Bro B, Leroy-Cotteau A, Mazingue F et al. Invasive fungal infections: epidemiology and analysis of antifungal prescriptions in onco-haematology. J Clin Pharm Ther 2011; 36: 152-60.
34. Knoke M, Bernhardt H, Schwesinger G. Is there a need for autopsies in the management of fungal disease? Mycoses 2008; 51: 291-300.
35. Lass-Flörl C, Gunsilius E, Gastl G et al. Clinical evaluation of Aspergillus-PCR for detection of invasive aspergillosis in immunosuppressed patients. Mycoses 2005; 48 Suppl 1: 12-7.
36. Sanguinetti M, Posteraro B, Pagano L et al. Comparison of real-time PCR, conventional PCR, and galactomannan antigen detection by enzyme-linked immunosorbent assay using bronchoalveolar lavage fluid samples from hematology patients for diagnosis of invasive pulmonary aspergillosis. J Clin Microbiol 2003; 41: 3922-5.