Targeted codon optimization improves translational fidelity for an Fc fusion protein

Biotechnology and Bioengineering

Volumn 109, Issue 11, 2012, Pages 2770-2777

  Hutterer, Katariina M ^a   Zhang, Zhongqi ^a   Michaels, Mark Leo ^a   Belouski, Ed ^a   Hong, Robert W ^a   Shah, Bhavana ^a   Berge, Mark ^b   Barkhordarian, Hedieh ^a   Le, Eleanor ^a   Smith, Steve ^a   Winters, Dwight ^a   Abroson, Frank ^a   Hecht, Randy ^a   Liu, Jennifer ^a  

^a AMGEN INC   (United States)

^b MEDIMMUNE   (United States)

Author keywords

Codon; E. coli; Fusion protein; Low fidelity translation; Misincorporation

Indexed keywords

AMINO ACID SEQUENCE; BIOPHARMACEUTICAL PRODUCTS; CODON; CODON OPTIMIZATION; E. COLI; FERMENTATION CONDITIONS; FULL OPTIMIZATION; FUSION PROTEINS; HIGH QUALITY; MISINCORPORATION; PROTEIN QUALITY; TRANSGENICS;

AMINO ACIDS; ERRORS; ESCHERICHIA COLI; FERMENTATION; PROTEINS;

OPTIMIZATION;

ARGININE; GLUTAMIC ACID; GLYCINE; HYBRID PROTEIN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CODON; DNA SEQUENCE; ELECTROSPRAY; ESCHERICHIA COLI; FERMENTATION; NONHUMAN; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

BIOTECHNOLOGY; CODON; ESCHERICHIA COLI; FERMENTATION; IMMUNOGLOBULIN FC FRAGMENTS; METABOLIC ENGINEERING; PROTEIN BIOSYNTHESIS; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI;

EID: 84866750386     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24555     Document Type: Article

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