Performance evaluation of 3D polystyrene 96-well plates with human neural stem cells in a calcium assay

Journal of Laboratory Automation

Volumn 17, Issue 4, 2012, Pages 284-292

  Lai, Yinzhi ^a   Kisaalita, William S ^a  

^a UNIVERSITY OF GEORGIA   (United States)

Author keywords

3D cell culture; Cell based assay; Drug discovery; HTS; Progenitor cell; Scaffold; Voltage gated calcium channel

Indexed keywords

CALCIUM; CELL CULTURE; DRUG PRODUCTS; POLYSTYRENES; SCAFFOLDS; SCANNING ELECTRON MICROSCOPY; STEM CELLS; WELDING;

3-D CELL CULTURE; CALCIUM CHANNELS; CELL-BASED ASSAYS; DRUG DISCOVERY; PROGENITOR CELL;

SCAFFOLDS (BIOLOGY);

FLUORESCENT DYE; LAMININ; MOLECULAR SCAFFOLD; POLYORNITHINE; POLYSTYRENE; SCLEROPROTEIN; VOLTAGE GATED POTASSIUM CHANNEL; CALCIUM; POLYSTYRENE DERIVATIVE;

3D CELL CULTURE PLATE; ANALYTICAL PARAMETERS; ARTICLE; BIOMEDICAL TECHNOLOGY ASSESSMENT; CALCIUM CELL LEVEL; CALCIUM DETERMINATION; CALCIUM SIGNALING; CELL CULTURE EQUIPMENT; CELL STRUCTURE; COMPARATIVE STUDY; CONTROLLED STUDY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; LABORATORY AUTOMATION; MATERIAL COATING; MICROPLATE READER; NEURAL STEM CELL; POROSITY; RESERVOIR; SCANNING ELECTRON MICROSCOPE; SCANNING ELECTRON MICROSCOPY; Z' FACTOR; CULTURE TECHNIQUE; DEVICES; EVALUATION; METABOLISM; METHODOLOGY; MICROSCOPY; PHYSIOLOGY;

CALCIUM; CELL CULTURE TECHNIQUES; EQUIPMENT AND SUPPLIES; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MICROSCOPY; NEURAL STEM CELLS; POLYSTYRENES;

EID: 84866712166     PISSN: 22110682     EISSN: 22110690     Source Type: Journal    
DOI: 10.1177/2211068212442503     Document Type: Article

References (13)

1. Murugan, R.; Ramakrishna, S. Nano-Featured Scaffolds for Tissue Engineering: A Review of Spinning Methodologies. Tissue Eng. 2006, 12, 435-447.
2. Cheng, K.; Lai, Y.; Kisaalita, W. S. Three-Dimensional Polymer Scaffolds for High Throughput Cell-Based Assay Systems. Biomaterials 2008, 29, 2802-2812.
3. Knight, E.; Murray, B.; Carnachan, R.; Przyborski, S. Alvetex®: Polystyrene Scaffold Technology for Routine Three Dimensional Cell Culture. Methods Mol. Biol. 2011, 695, 323-340.
4. Dhara, S. K.; Hasneen, K.; Machacek, D. W.; Boyd, N. L.; Rao, R. R.; Stice, S. L. Human Neural Progenitor Cells Derived from Embryonic Stem Cells in Feeder-Free Cultures. Differentiation 2008, 76, 454-464.
5. Breier, J. M.; Gassmann, K.; Kayser, R.; Stegeman, H.; De Groot, D.; Fritsche, E.; Shafer, T. J. Neural Progenitor Cells as Models for High-Throughput Screens of Developmental Neurotoxicity: State of the Science. Neurotoxicol. Teratol. 2010, 32, 4-15.
6. Mathie, A. Ion Channels as Novel Therapeutic Targets in the Treatment of Pain. J. Pharm. Pharmacol. 2010, 62, 1089-1095.
7. Macphee, C. New Advances in Drug Discovery. Expert Opin. Investig. Drugs 2004, 13, 1213-1215.
8. Molokanova, E.; Savchenko, A. Bright Future of Optical Assays for Ion Channel Drug Discovery. Drug Discov. Today 2008, 13, 14-22.
9. Arcangeli, A.; Crociani, O.; Lastraioli, E.; Masi, A.; Pillozzi, S.; Becchetti, A. Targeting Ion Channels in Cancer: A Novel Frontier in Antineoplastic Therapy. Curr. Med. Chem. 2009, 16, 66-93.
10. Varga, Z.; Hajdu, P.; Panyi, G.; Gaspar, R.; Krasznai, Z. Involvement of Membrane Channels in Autoimmune Disorders. Curr. Pharm. Des. 2007, 13, 2456-2468.
11. Zheng, W.; Spencer, R. H.; Kiss, L. High Throughput Assay Technologies for Ion Channel Drug Discovery. Assay Drug Dev. Technol. 2004, 2, 543-552.
12. Zhang, J. H.; Chung, T. D.; Oldenburg, K. R. A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assay. J. Biomol. Screen. 1999, 4, 67-73.
13. Invitrogen. Fluo-4 AM & Fluo-4 NW Calcium Indicator. http:// www.invitrogen.com/site/us/en/home/Products-and-Services/ Applications/Drug-Discovery/Target-and-Lead-Identificationand-Validation/g-protein_coupled_html/cell-based-secondmessenger-assays/fluo-4-am-fluo-4-nw-calcium-indicators. html. Accessed October 7, 2011.