Both the AOX1 promoter and the FLD1 promoter work together in a Pichia pastoris expression vector

World Journal of Microbiology and Biotechnology

Volumn 25, Issue 10, 2009, Pages 1779-1783

  Duan, Huiming ^a   Umar, Sirajo ^a   Hu, Yiping ^a   Chen, Jinchun ^a  

^a BEIJING UNIVERSITY OF CHEMICAL TECHNOLOGY   (China)

Author keywords

Expression vector; Pichia pastoris; The AOX1 promoter; The FLD1 promoter

Indexed keywords

ALCOHOL OXIDASE 1 PROMOTER; EXPRESSION VECTORS; EXTRACELLULAR PROTEINS; FORMALDEHYDE DEHYDROGENASE; GREEN FLUORESCENT PROTEIN; INDUCIBLE PROMOTER; PICHIA PASTORIS; PROTEIN EXPRESSIONS;

METHANOL; VECTORS;

PROTEINS;

PICHIA PASTORIS;

EID: 71349086113     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-009-0077-4     Document Type: Article

References (6)

1. Cregg JM, Claremont CA (2004) Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1. United States Patent 6730499B1.
2. Lin Cereghino GP, Lin Cereghino J, Sunga AJ, Johnson MA, Lim M, Gleeson MA, Cregg JM (2001) New selectable marker/auxotrophic host strain combinations for molecular genetic manipulation of Pichia pastoris. Gene 263: 159-169. doi: 10. 1016/S0378-1119(00)00576-X.
3. Myllyharju J, Nokelainen M, Vuorela A, Kivirikko KI (2000) Expression of recombinant human type I-III collagens in the yeast Pichia pastoris. Biochem Soc Trans 28: 353-357. doi: 10. 1042/0300-5127: 0280353.
4. Shen S, Sulter G, Jeffries TW, Cregg JM (1998) A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris. Gene 216: 93-102. doi: 10. 1016/S0378-1119(98)00315-1.
5. Toman PD, Chisholm G, McMullin H, Giere LM, Olsen DR, Kovach RJ, Leigh SD, Fong BE, Chang R, Daniels GA, Berg RA, Hitzeman RA (2000) Production of recombinant human type I procollagen trimers using a four-gene expression system in the yeast Saccharomyces cerevisiae. J Biol Chem 275: 23303-23309. doi: 10. 1074/jbc. M002284200.
6. Tschopp JF, Brust PF, Cregg JM, Stillman CA, Gingeras TR (1987) Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucleic Acids Res 15: 3859-3876. doi: 10. 1093/nar/15. 9. 3859.