Construction of in vitro transcription system for Corynebacterium glutamicum and its use in the recognition of promoters of different classes

Applied Microbiology and Biotechnology

Volumn 96, Issue 2, 2012, Pages 521-529

  Holátko, Jirí ^a   Šilar, Radoslav ^a   Rabatinová, Alžbeta ^a   Šanderová, Hana ^a   Halada, Petr ^a   Nešvera, Jan ^a   Krásný, Libor ^a   Pátek, Miroslav ^a  

^a INSTITUTE OF MICROBIOLOGY   (Czech Republic)

Author keywords

Corynebacterium glutamicum; In vitro transcription; Promoter; RNA polymerase; Sigma factor

Indexed keywords

CORYNEBACTERIUM GLUTAMICUM; IN-VITRO; PROMOTER; RNA POLYMERASE; SIGMA FACTOR;

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; CLONING; ESCHERICHIA COLI; GENES; PURIFICATION; RNA;

TRANSCRIPTION;

AMINO ACID; BACTERIAL PROTEIN; REGULATOR PROTEIN; RNA POLYMERASE; SIGMA FACTOR;

AMINO ACID; BACTERIUM; NUMERICAL MODEL; PLASMID; POLYMERASE CHAIN REACTION; PROTEIN; RNA;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CORYNEBACTERIUM GLUTAMICUM; DNA TEMPLATE; GENE OVEREXPRESSION; GENE SEQUENCE; GENETIC TRANSCRIPTION; IN VITRO STUDY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR CLONING; MOLECULAR RECOGNITION; NONHUMAN; PROMOTER REGION; REACTION OPTIMIZATION; RPOC GENE; SIGA GENE; SIGH GENE; TRANSCRIPTION REGULATION;

BASE SEQUENCE; CORYNEBACTERIUM GLUTAMICUM; DNA-DIRECTED RNA POLYMERASES; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC TECHNIQUES; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS, GENETIC; PROTEIN BINDING; SIGMA FACTOR; SPECIES SPECIFICITY; TRANSCRIPTION, GENETIC;

ACTINOBACTERIA; BACTERIA (MICROORGANISMS); CORYNEBACTERIUM GLUTAMICUM; ESCHERICHIA COLI;

EID: 84866495062     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4336-1     Document Type: Article

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