RT-qPCR analysis of putative beer-spoilage gene expression during growth of Lactobacillus brevis BSO 464 and Pediococcus claussenii ATCC BAA-344 T in beer

Applied Microbiology and Biotechnology

Volumn 96, Issue 2, 2012, Pages 461-470

  Bergsveinson, Jordyn ^a   Pittet, Vanessa ^a   Ziola, Barry ^a  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

Author keywords

Beer spoilage; Gene expression; Hop resistance; Lactic acid bacteria; Reference gene normalization; RT qPCR

Indexed keywords

ANTI-MICROBIAL EFFECTS; BEER SPOILAGE; DATA NORMALIZATION; GENETIC EXPRESSIONS; LACTIC ACID BACTERIA; LACTOBACILLUS BREVIS; PEDIOCOCCUS; REFERENCE GENE NORMALIZATION; REVERSE TRANSCRIPTION QUANTITATIVE-PCR; RT-QPCR;

ACID RESISTANCE; BACTERIA; GENE EXPRESSION; GENES; LACTIC ACID; POLYMERASE CHAIN REACTION; SPOILAGE; TRANSCRIPTION;

BEER;

ANTIMICROBIAL ACTIVITY; ASSESSMENT METHOD; BACTERIUM; GENE EXPRESSION; GENETIC ANALYSIS; GROWTH RATE; POLLUTION EFFECT; POLYMERASE CHAIN REACTION;

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BEER; BLPA BACTERIAL GENE; BSRA BACTERIAL GENE; FABD BACTERIAL GENE; FUSA BACTERIAL GENE; GENE EXPRESSION; GMK BACTERIAL GENE; GYRA BACTERIAL GENE; HITA BACTERIAL GENE; HORA BACTERIAL GENE; HORB BACTERIAL GENE; HORC BACTERIAL GENE; ILES BACTERIAL GENE; LACTOBACILLUS BREVIS; LDHA BACTERIAL GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; PCRA BACTERIAL GENE; PEDIOCOCCUS; PEDIOCOCCUS CLAUSSENII; PFKA BACTERIAL GENE; PROC BACTERIAL GENE; RECA BACTERIAL GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; RPOB BACTERIAL GENE;

BACTERIAL PROTEINS; BEER; DNA PRIMERS; FOOD CONTAMINATION; GENE EXPRESSION REGULATION, BACTERIAL; LACTOBACILLUS BREVIS; PEDIOCOCCUS; REAL-TIME POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); HUMULUS; LACTOBACILLUS BREVIS; PEDIOCOCCUS CLAUSSENII;

EID: 84866490508     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4334-3     Document Type: Article

References (36)

1. Bamforth CW (2006) Scientific principles of malting and brewing. American Society of Brewing Chemists, St. Paul
2. Behr J, Gänzle MG, Vogel RF (2006) Characterization of a highly hopresistant Lactobacillus brevis strain lacking hop transport. Appl Environ Microbiol 72:6483-6492 (Pubitemid 44582884)
3. Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT (2009) The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55:611-622
4. De Man JC, Rogosa M, Sharpe ME (1960) A medium for the cultivation of Lactobacilli. J Appl Bacteriol 23:130-135
5. Derveaux S, Vandesompele J, Hellemans J (2010) How to do successful gene expression analysis using real-time PCR. Methods 50:227-230
6. Desroche N, Beltramo C, Guzzo J (2005) Determination of an internal control to apply reverse transcription quantitative PCR to study stress response in the lactic acid bacterium Oenococcus oeni. J Microbiol Methods 60:325-333 (Pubitemid 40093058)
7. Dheda K, Huggett JF, Bustin SA, Johnson MA, Rook G, Zumla A (2004) Validation of housekeeping genes for normalizing RNA expression in real-time PCR. BioTechniques 37:112-119 (Pubitemid 38902668)
8. Dobson CM, Deneer H, Lee S, Hemmingsen S, Glaze S, Ziola B (2002) Phylogenetic analysis of the genus Pediococcus, including Pediococcus claussenii sp. nov., a novel lactic acid bacterium isolated from beer. Int J Syst Evol Microbiol 52:2003-2010 (Pubitemid 36004178)
9. Fiocco D, Crisetti E, Capozzi V, Spano G (2008) Validation of an internal control gene to apply reverse transcription quantitative PCR to study heat, cold, and ethanol stresses in Lactobacillus plantarum. World J Microbiol Biotechnol 24:899-902 (Pubitemid 351585268)
10. Fujii T, Nakashima K, Hayashi N (2005) Random amplified polymorphic DNA-PCR based cloning of markers to identify the beer-spoilage strains of Lactobacillus brevis, Pediococcus damnosus, Lactobacillus collinoides and Lactobacillus coryniformis. J Appl Microbiol 98:1209-1220 (Pubitemid 40562647)
11. Haakensen MC, Butt L, Chaban B, Deneer H, Ziola B, Dowgiert T (2007) horA-specific real-time PCR for detection of beer-spoilage lactic acid bacteria. J Am Soc Brew Chem 65:157-165 (Pubitemid 47221827)
12. Haakensen M, Schubert A, Ziola B (2008) Multiplex PCR for putative Lactobacillus and Pediococcus beer-spoilage genes and ability of gene presence to predict growth in beer. J Am Soc Brew Chem 66:63-70 (Pubitemid 351632709)
13. Haakensen M, Pittet V, Morrow K, Schubert A, Ferguson J, Ziola B (2009) Ability of novel ATP-binding cassette multidrug resistance genes to predict growth of Pediococcus isolates in beer. J Am Soc Brew Chem 67:170-176
14. Hayashi N, Ito M, Horiike S, Taguchi H (2001) Molecular cloning of a putative divalent-cation transporter gene as a new genetic marker for the identification of Lactobacillus brevis strains capable of growing in beer. Appl Microbiol Biotechnol 55:596-603 (Pubitemid 32480547)
15. Hellemans J, Mortier G, De Paepe A, Speleman F, Vandesompele J (2007) qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biol 8:R19
16. Huggett J, Dheda K, Bustin S, Zumla A (2005) Real-time RT-PCR normalisation; strategies and considerations. Genes Immun 6:279-284 (Pubitemid 40903399)
17. Iijima K, Suzuki K, Ozaki K, Yamashita H (2006) horC confers beerspoilage ability on hop-sensitive Lactobacillus brevis ABBC45cc. J Appl Microbiol 100:1282-1288 (Pubitemid 43725692)
18. Kandler O (1983) Carbohydrate metabolism in lactic acid bacteria. Anton Leeuw 49:209-224 (Pubitemid 13015417)
19. Marco ML, Kleerebezem M (2008) Assessment of real-time RT-PCR for quantification of Lactobacillus plantarum gene expression during stationary phase and nutrient starvation. J Appl Microbiol 104:587-594
20. Pittet V, Abegunde T, Marfleet T, Haakensen M, Morrow K, Jayaprakash T, Schroeder K, Trost B, Byrns S, Bergsveinson J, Kusalik A, Ziola B (2012) Complete genome sequence of the beer spoilage organism Pediococcus claussenii ATCC BAA-344T. J Bacteriol 194:1271-1272
21. Preissler P, Behr J, Vogel RF (2010) Detection of beer-spoilage Lactobacillus brevis strains by reduction of resazurin. J Inst Brew 116:399-404
22. Priest FG, Campbell I (2003) Brewing microbiology. Kluwer Academic/Plenum Publishers, New York
23. Sakamoto K, Konings WN (2003) Beer spoilage bacteria and hop resistance. Int J Food Microbiol 89:105-124 (Pubitemid 37420022)
24. Sakamoto K, van Veen HW, Saito H, Kobayashi H, Konings WN (2002) Membrane-bound ATPase contributes to hop resistance of Lactobacillus brevis. Appl Environ Microbiol 68:5374-5378 (Pubitemid 35265670)
25. Sami M, Yamashita H, Hirono T, Kadokura H, Kitamoto K, Yoda K, Yamasaki M (1997) Hop-resistant Lactobacillus brevis contains a novel plasmid harboring a multidrug resistance-like gene. J Ferment Bioeng 84:1-6 (Pubitemid 27392178)
26. Simpson WJ (1993a) Studies on the sensitivity of lactic acid bacteria to hop bitter acids. J Inst Brew 99:405-411
27. Simpson WJ (1993b) Ionophoric action of trans-isohumulone on Lactobacillus brevis. J Gen Microbiol 139:1041-1045 (Pubitemid 23164475)
28. Simpson WJ, Fernandez JL (1992) Selection of beer-spoilage lactic acid bacteria and induction of their ability to grow in beer. Lett Appl Microbiol 14:13-16
29. SimpsonWJ, Fernandez JL (1994)Mechanism of resistance of lactic acid bacteria to trans-isohumulone. J Am Soc Brew Chem 52:9-11
30. Suzuki K, Sami M, Kadokura H, Nakajima H, Kitamoto K (2002) Biochemical characterization of horA-independent hop resistance mechanism in Lactobacillus brevis. Int J Food Microbiol 76:223-230 (Pubitemid 34327248)
31. Suzuki K, Koyanagi M, Yamashita H (2004) Genetic characterization of non-spoilage variant isolated from beer-spoilage Lactobacillus brevis ABBC45. J Appl Microbiol 96:946-953 (Pubitemid 38582140)
32. Suzuki K, Iijima K, Ozaki K, Yamashita H (2005) Isolation of a hopsensitive variant of Lactobacillus lindneri and identification of genetic markers for beer spoilage ability of lactic acid bacteria. Appl Environ Microbiol 71:5089-5097 (Pubitemid 41338235)
33. Suzuki K, Iijima K, Sakamoto K, Sami M, Yamashita H (2006) A review of hop resistance in beer spoilage lactic acid bacteria. J Inst Brew 112:173-191 (Pubitemid 44326600)
34. Theis T, Skurray RA, Brown MH (2007) Identification of suitable internal controls to study expression of a Staphylococcus aureus multidrug resistance system by quantitative real-time PCR. J Microbiol Methods 70:355-362 (Pubitemid 47198142)
35. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3:0034.1-0034.11
36. Willems E, Leyns L, Vandesompele J (2008) Standardization of realtime PCR gene expression data from independent biological replicates. Anal Biochem 379:127-129