Alanine scanning mutagenesis of a high-affinity nitrate transporter highlights the requirement for glycine and asparagine residues in the two nitrate signature motifs

Biochemical Journal

Volumn 447, Issue 1, 2012, Pages 35-42

  Unkles, Shiela E ^a   Karabika, Eugenia ^a,d   Symington, Vicki F ^a   Cecile, Jennifer L ^b   Rouch, Duncan A ^c   Akhtar, Naureen ^a   Cromer, Brett A ^c   Kinghorn, James R ^a  

^a UNIVERSITY OF ST ANDREWS   (United Kingdom)

^b APPALACHIAN STATE UNIVERSITY   (United States)

^c RMIT UNIVERSITY   (Australia)

^d UNIVERSITY OF IOANNINA   (Greece)

Author keywords

Alanine scanning; Helix packing; Major facilitator superfamily (MFS); Nitrate transporter; Structural model

Indexed keywords

ALANINE; ARGININE; ASPARAGINE; GLYCINE; NITRATE; NITRATE TRANSPORTER; NITRATE TRANSPORTER NRTA; NITRATE TRANSPORTER NS1; NITRATE TRANSPORTER NS2; UNCLASSIFIED DRUG;

ALANINE SCANNING MUTAGENESIS; AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS NIDULANS; CYTOPLASM; ENZYME SUBSTRATE COMPLEX; GENETIC ANALYSIS; MICROBIAL GROWTH; MOLECULAR MODEL; MUTAGENESIS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SECONDARY STRUCTURE;

ALANINE; AMINO ACID MOTIFS; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANION TRANSPORT PROTEINS; ASPARAGINE; ASPERGILLUS NIDULANS; BINDING SITES; CONSERVED SEQUENCE; FUNGAL PROTEINS; GLYCINE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; NITRATES; PHENOTYPE; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY;

EMERICELLA NIDULANS;

EID: 84866399105     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20120631     Document Type: Article

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