Splicing by over-lap extension by PCR using asymmetric amplification: An improved technique for the generation of hybrid proteins of immunological interest

Gene

Volumn 186, Issue 1, 1997, Pages 29-35

  Warrens, Anthony N ^a   Jones, Michael D ^b   Lechler, Robert I ^a  

^a HAMMERSMITH HOSPITAL   (United Kingdom)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

DNA polymerase; Gene shuffling; Major histocompatibility complex; MHC; Oligonucleotide

Indexed keywords

COMPLEMENTARY DNA; DNA FRAGMENT; DNA POLYMERASE; GLYCOPROTEIN; HYBRID PROTEIN; RESTRICTION ENDONUCLEASE; HISTOCOMPATIBILITY ANTIGEN; HLA ANTIGEN; RECOMBINANT PROTEIN;

ANTIGEN RECOGNITION; ARTICLE; GENE AMPLIFICATION; GENE MUTATION; GENETIC ENGINEERING; HUMAN; IMMUNOGENETICS; MAJOR HISTOCOMPATIBILITY COMPLEX; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA SPLICING; STRUCTURE ACTIVITY RELATION; TECHNIQUE; ANIMAL; GENETICS; METHODOLOGY; MUTATION; PROTEIN ENGINEERING;

ANIMALS; HISTOCOMPATIBILITY ANTIGENS; HLA ANTIGENS; MAJOR HISTOCOMPATIBILITY COMPLEX; MICE; MUTATION; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; RNA SPLICING;

EID: 0031579372     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00674-9     Document Type: Article

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