Cloning and expression of a novel xylanase gene (Auxyn11D) from Aspergillus usamii E001 in Pichia pastoris

Applied Biochemistry and Biotechnology

Volumn 167, Issue 8, 2012, Pages 2198-2211

  Zhang, Huimin ^a   Wu, Minchen ^a   Li, Jianfang ^a   Gao, Shujuan ^a   Yang, Yanjun ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Aspergillus usamii; Cloning; Expression; Pichia pastoris; Xylanase

Indexed keywords

AMINO ACID SEQUENCE; CDNA ENDS; CDNA SEQUENCE; CLONING AND EXPRESSION; EXPRESSION; GLYCOSIDE HYDROLASES; GLYCOSYLATED; OPEN READING FRAME; P. PASTORIS; PH RANGE; PICHIA PASTORIS; SDS-PAGE; SHAKE FLASK TESTS; SIGNAL PEPTIDE; UNTRANSLATED REGIONS; XYLANASES;

AMINO ACIDS; ASPERGILLUS; CLONING; ENCODING (SYMBOLS); MANGANESE; MANGANESE COMPOUNDS; METAL IONS; PEPTIDES; YEAST;

GENE EXPRESSION;

CALCIUM ION; COMPLEMENTARY DNA; DNA FRAGMENT; EDETIC ACID; GLYCOSIDASE; MANGANESE; METAL ION; PROTEIN AUXYN11D; RECOMBINANT PROTEIN; SIGNAL PEPTIDE; UNCLASSIFIED DRUG; XYLAN; XYLAN ENDO 1,3 BETA XYLOSIDASE;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS; ASPERGILLUS USAMII; AUXYN11D GENE; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME STABILITY; FUNGAL GENE; GENE AMPLIFICATION; GENE EXPRESSION; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN FAMILY; PROTEIN PURIFICATION; PROTEIN SECRETION; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ASPERGILLUS; BASE SEQUENCE; CLONING, MOLECULAR; ENDO-1,4-BETA XYLANASES; ENZYME STABILITY; FUNGAL PROTEINS; GENE EXPRESSION; MOLECULAR SEQUENCE DATA; PICHIA; SEQUENCE ALIGNMENT;

ASPERGILLUS USAMII; PICHIA PASTORIS;

EID: 84866395854     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-012-9757-x     Document Type: Article

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