Production of alkaliphilic, halotolerent, thermostable cellulase free xylanase by Bacillus halodurans PPKS-2 using agro waste: Single step purification and characterization

World Journal of Microbiology and Biotechnology

Volumn 28, Issue 1, 2012, Pages 183-192

  Prakash, P ^a,c   Jayalakshmi, S K ^a   Prakash, B ^b   Rubul, M ^b   Sreeramulu, K ^c  

^a UNIVERSITY OF AGRICULTURAL SCIENCES   (India)

^b TATA INSTITUTE OF FUNDAMENTAL RESEARCH   (India)

^c GULBARGA UNIVERSITY   (India)

Author keywords

Alkaliphilic; Bacillus halodurans PPKS 2; Corn husk; Endo 1,4 xylanase; Halotolerent; Thermostable

Indexed keywords

ALKALIPHILIC; BACILLUS HALODURANS PPKS-2; CORN HUSK; HALOTOLERENT; THERMOSTABLE;

AMINO ACIDS; BACILLI; BACTERIOLOGY; CHROMATOGRAPHIC ANALYSIS; ENZYME ACTIVITY; SODIUM; SODIUM CHLORIDE; SUGARS;

PURIFICATION;

CARBON; CELLULASE; ENDO 1,4 BETA XYLANASE; NITROGEN;

AGRICULTURE; AMINO ACID SEQUENCE; ARTICLE; BACILLUS; BIOSYNTHESIS; ENZYME STABILITY; ENZYMOLOGY; GENETICS; GROWTH, DEVELOPMENT AND AGING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INDUSTRIAL WASTE; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR GENETICS; PH; SALINITY; SEQUENCE HOMOLOGY; TANDEM MASS SPECTROMETRY; TEMPERATURE;

AGRICULTURE; AMINO ACID SEQUENCE; BACILLUS; CARBON; CELLULASE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENDO-1,4-BETA XYLANASES; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; INDUSTRIAL WASTE; KINETICS; MOLECULAR SEQUENCE DATA; NITROGEN; SALINITY; SEQUENCE HOMOLOGY, AMINO ACID; TANDEM MASS SPECTROMETRY; TEMPERATURE;

BACILLUS HALODURANS; TRITICUM AESTIVUM SUBSP. SPELTA; ZEA MAYS;

EID: 84655169750     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-011-0807-2     Document Type: Article

References (46)

1. Anuradha P, Vijayalakshmi K, Prasanna ND, Sridevi K (2007) Production and properties of alkaline xylanases from Bacillus sp. isolated from sugarcane fields. Curr Sci 90: 1283-1286.
2. Bajpai P (1999) Application of enzymes in the pulp and paper industry. Biotechnol Prog 15: 147-157.
3. Battan B, Sharma J, Dhiman SS, Kuhad RC (2007) Enhanced production of cellulase-free thermostable xylanse by Bacillus pumilus ASH and its potential application in paper industry. Enzyme Microb Technol 41: 733-739.
4. Beg QK, Bhushan B, Kapoor M, Hoondal GS (2000) Enhanced production of a thermostable xylanase from streptomyces sp, QG-11-3 and its application in biobleaching of eucalyptus kraft pulp. Enzyme Microbiol Technol 27: 459-466.
5. Beg QK, Kapoor M, Mahajan L, Hoondal GS (2001) Microbial xylanases and their industrial applications-a review. Appl Microbiol Biotechnol 56: 326-338.
6. Cardoso OAV, Filho EXF (2003) Purification and characterization of a novel cellulase-free xylanase from Acrophialophora nainiana. FEMS Microbiol Lett 223: 309-314.
7. Collins T, Gerday C, Feller G (2005) Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiol Rev 29: 3-23.
8. Dhillon A, Khanna S (2000) Production of a thermostable alkalitolerent xylanase from Bacillus circulans AB 16 grown on wheat straw. World J Microbiol Biotechnol 16: 325-327.
9. Gallardo O, Diaz P, Pastor FI (2004) Cloning and characterization of xylanase A from the strain Bacillus sp. BP-7: comparison with alkaline pI-low molecular weight xylanases of family 11. Curr Microbiol 48: 276-279.
10. Georis J, Giannotta F, De Buyl E, Granier B, Frere JM, De Buyl E (2000) Purification and properties of three endo-beta-1, 4-xylanases produced by Streptomyces sp. strain S38 which differ in their ability to enhance the bleaching of kraft pulps. Enzyme Microb Technol 26: 178-186.
11. Gessesse A, Mamo G (1999) High-level xylanase production by an alkaliphilic Bacillus sp. by using solid-state fermentation. Enzyme Microb Technol 25: 68-72.
12. Jalal A, Rashid N, Rasool N, Akhtar M (2009) Gene cloning and characterization of a xylanase from a newly isolated Bacillus subtilis strain R5. J Biosci Bioeng 107: 360-365.
13. Khandeparkar R, Bhosle N (2006a) Purification and characterization of thermoalkalophilic xylanase isolated from the Enterobacter sp. MTCC 5112. Res Microbiol 157: 315-325.
14. Khandeparkar R, Bhosle N (2006b) Isolation, purification and characterization of the xylanase produced by Arthrobacter sp. MTCC 5214 when grown in solid-state fermentation. Enzyme Microb Technol 39: 732-742.
15. Khasin A, Shoham AY (1993) Purification and characterization of a thermostable xylanase from Bacillus stearothermophilus T-6. Appl Environ Microbiol 59: 1725-1730.
16. Laemmli UK (1970) Cleavage of structural proteins during assembly of head of bacteriophage. T4. Nature 227: 680-685.
17. Lopez-Fernandez CL, Rodriguez J, Ball AS, Copa-Patino JL, Perez-Leblic MI, Arias ME (1998) Application of the affinity binding of xylanases to oat-spelt xylan in the purification of endoxylanase CM-2 from Streptomyces chattanoogensis CECT 3336. Appl Microbiol Biotechnol 50: 284-287.
18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193(1): 265-275.
19. Mamo G, Hatti-kaul R, Mattiasson B (2006) A thermostable alkaline active endo-β1-4-xylanase from Bacillus halodurans S7. Purification and characterization. Enzyme Microb Technol 39: 1492-1498.
20. Miller G (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal Chem 31: 426-428.
21. Nakamura S, Wakabayashi K, Nakai R, Ano R, Horikoshi K (1993) Purification and some properties of an alkaline xylanase from alkaliphilic Bacillus sp. strain 41M-1. Appl Environ Microbiol 59: 2311-2316.
22. Nath D, Rao M (2000) pH dependent conformational and structural changes of xylanase from an alkalophilic thermophilic Bacillus sp. (NCIM59). Enzyme Microb Technol 28: 397-403.
23. Palonen H, Tenkanen M, Linder M (1999) Dynamic interaction of Trichoderma reeseicellobiohydrolases Cel6A and Cel7A and cellulose at equilibrium and during hydrolysis. Appl Environ Microbiol 65: 5229-5233.
24. Perkins DN, Pappian DJ, Creasy DM, Cottrell JS (1999) Probability-based protein identification by searching sequences database using mass spectroscopy data. Electrophoresis 20: 3551-3567.
25. Prakash P, Jayalakshmi SK, Sreeramulu K (2010) Production of keratinase by free and immobilized cells of Bacillus halodurans strain PPKS-2 partial characterization and its application in feather degradation and dehairing of the goat skin. Appl Biochem Biotechnol 160: 1909-1920.
26. Qureshy AF, Khan LA, Khanna S (2000) Expression of Bacillus circulans Teri-42 xylanase gene in Bacillus subtilis. Enzyme Microb Technol 27: 227-233.
27. Rani DS, Nand K (2000) Production of thermostable cellulase-free xylanase by Clostridium absonum CFR-702. Process Biochem 36: 255-262.
28. Ratanakhanokchai K, Kyu KL, Tanticharoen M (1999) Purification and properties of a xylan-binding endoxylanase from alkaliphilic Bacillus sp. Strain K-1. Appl Environ Microbiol 65: 694-697.
29. Saha CB (2003) Hemicellulosic bioconversion. J Ind Microbiol Biotechnol 30: 279-291.
30. Sanghi A, Garg N, Kuhar K, Kuhad RC, Gupta VK (2009) Enhanced production of cellulase-free xylanase by alkalophilic Bacillus subtilis ASH and its application in biobleaching of kraft pulp. Bio Resources 4: 1109-1129.
31. Santos ESD, Guirardello R, Franco TT (2002) Preparative chromatography of xylanase using expanded bed adsorption. J Chromatogr 944: 217-224.
32. Silveira FQP, Melo IS, Filho EXF (1997) Carbohydrate-hydrolysing enzyme activity production by solid-state cultures of Trichoderma harzianum strains. Rev Microbiol 28: 152-156.
33. Singh S, Madlala AM, Prior BA (2003) Thermomyces lanuginosus: properties of strains and their hemicellulases. FEMS Microbiol Rev 27: 3-16.
34. Subramaniyan S, Prema P (2000) Cellulase free xylanases from Bacillus and other microorganisms. FEMS Microbiol Lett 183: 1-7.
35. Subramaniyan S, Sandhia GS, Prema P (2001) Control of xylanase production without protease activity in Bacillus sp. by selection of nitrogen source. Biotechnol Lett 23: 369-371.
36. Sun Y, Cheng J (2002) Hydrolysis of lignocellulose materials for ethanol production. A review. Biores Technol 83: 1-11.
37. Tabernero C, Sanchez-Torres J, Perez P, Santamaria RI (1995) Cloning and DNA sequencing of xyaA, a gene encoding an endo-beta-1, 4-xylanase from an alkalophilic Bacillus strain (N137). Appl Environ Microbiol 61: 2420-2424.
38. Techaapiakoon C, Kyu KL, Ratanakhnokchai K (2006) Purification of xylanase from alkaliphilic Bacillus sp. K-8 by using corn husk column. Process Biochem 41: 2441-2445.
39. Techapun C, Sinsuwongwat S, Poosaran N, Watanabe M, Sasaki K (2001) Production of a cellulase-free xylanase from agricultural waste materials by a thermotolerant Streptomyces sp. Biotechnol Lett 23: 1685-1689.
40. Teotia S, Khare SK, Gupta MN (2001) An efficient purification process for sweet potato beta-amylase by affinity precipitation with alginate. Enzyme Microb Technol 28: 792-795.
41. Vafiadi C, Christakopoulos P, Topakas E (2010) Purification, characterization and mass spectrometric identification of two thermophilic xylanases from Sporotrichumthermophile. Process Biochem 45: 419-424.
42. Viikari L, Kantelinen A, Sundquist J, Linko M (1994) Xylanases in bleaching: from an idea to the industry. FEMS Microbiol Rev 13: 335-350.
43. Wang CY, Chan H, Lin HTY, Shyu YT (2010) Production, purification and characterisation of a novel halostable xylanase from Bacillus sp. NTU-06. Ann Appl Biol 156: 187-197.
44. Wejse PL, Ingvorsen K, Mortensen KK (2003) Xylanase production by a novel halophilic bacterium increased 20-fold by response surface methodology. Enzyme Microb Technol 32: 721-727.
45. Wu S, Liu B, Zhang X (2006) Characterization of a recombinant thermostable xylanase from deep-sea thermophilic Geobacillus sp. MT-1 in East Pacific. Appl Microbiol Biotechnol 72: 1210-1216.
46. Xia L, Len P (1999) Cellulose production by solid-state fermentation on lignocellulosic waste from the xylose industry. Process Biochem 34: 909-912.