Cloning and optimized expression of a GH-11 xylanase from Fusarium oxysporum in Pichia pastoris

New Biotechnology

Volumn 28, Issue 4, 2011, Pages 369-374

  Moukouli, Maria ^a   Topakas, Evangelos ^a   Christakopoulos, Paul ^a  

^a NATIONAL TECHNICAL UNIVERSITY OF ATHENS   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL OXIDASE PROMOTERS; CULTURE CONDITIONS; ENZYMATIC ACTIVITIES; ENZYME PRODUCTION; FAMILY 11; FLASK CULTURE; FUSARIUM OXYSPORUMS; GLYCOSYL HYDROLASES; INITIAL PH; METHANOL CONCENTRATION; METHYLOTROPHIC YEASTS; P. PASTORIS; PICHIA PASTORIS; SIGNAL PEPTIDE; XYLANASE ACTIVITY; XYLANASES;

CLONING; ENZYMES; GENES; METHANOL;

YEAST;

ALCOHOL OXIDASE; GLYCOSIDASE; GLYCOSYL HYDROLASE 11; METHANOL; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SYNTHESIS; FUNGAL GENOME; FUSARIUM OXYSPORUM; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PICHIA PASTORIS; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION;

CLONING, MOLECULAR; ENDO-1,4-BETA XYLANASES; FUSARIUM; PICHIA; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

FUSARIUM OXYSPORUM; PICHIA PASTORIS;

EID: 79958031759     PISSN: 18716784     EISSN: 18764347     Source Type: Journal    
DOI: 10.1016/j.nbt.2011.03.002     Document Type: Article

References (26)

1. Prade R.A. Xylanases: from biology to biotechnology. Biotechnol. Genet. Eng. Rev. 1995, 13:101-131.
2. Cantarel B.L., et al. The carbohydrate-active enzymes database (CAZy): an expert resource for glycogenomics. Nucleic Acids Res. 2009, 37:D233-238.
3. Collins T., et al. Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiol. Rev. 2005, 29:3-23.
4. Polizeli M.L., et al. Xylanases from fungi: properties and industrial applications. Mini-review. Appl. Microbiol. Biotechnol. 2005, 67:577-591.
5. Viikari L., et al. Xylanases in bleaching: from an idea to the industry. FEMS Microbiol. Rev. 1994, 113:335-350.
6. Buchert J., et al. The role of Trichoderma reesei xylanase and mannanase in treatment of softwood kraft pulp prior to bleaching. Holzforschung 1993, 47:473-478.
7. Baipai P. Application of enzymes in the pulp and paper industry. Biotechnol. Prog. 1999, 15:147-157.
8. Macauley-Patrick S., et al. Heterologous protein production using the Pichia pastoris expression system. Yeast 2005, 22:249-270.
9. Christakopoulos P., et al. Purification and characterization of two low molecular mass alkaline xylanases from Fusarium oxysporum F3. J. Biotech 1996, 51:181-189.
10. Christakopoulos P., et al. The alkaline xylanase III from Fusarium oxysporum F3 belongs to family F/10. Carbohydr. Res. 1997, 302:191-195.
11. Ruiz-Roldán M.C., et al. Two xylanase genes of the vascular wilt pathogen Fusarium oxysporum are differentially expressed during infection of tomato plants. Mol. Gen. Genet. 1999, 261:530-536.
12. Gómez-Gómez E., et al. Molecular characterization of a novel endo-β-1,4-xylanase gene from the vascular wilt fungus Fusarium oxysporum. Curr. Genet. 2001, 40:268-275.
13. Christakopoulos P., et al. Direct fermentation of cellulose to ethanol by Fusarium oxysporum. Enzyme Microb. Technol. 1989, 11:236-239.
14. Moukouli M., et al. Cloning, characterization and functional expression of an alkalitolerant type C feruloyl esterase from Fusarium oxysporum. Appl. Microbiol. Biotechnol. 2008, 79:245-254.
15. Sambrook J., et al. Molecular Cloning: A Laboratory Manual 1989, Cold Spring Harbor Laboratory Press, New York.
16. Higgins D.R., et al. Small vectors for expression based on dominant drug resistance with direct multicopy selection. Methods in Molecular Biology: Pichia Protocols 1998, 28-41. Humana Press, New Jersey. D.R. Higgins, J.M. Cregg (Eds.).
17. Bailey M.J., et al. Interlaboratory testing of methods for assay of xylanase activity. J. Biotechnol. 1992, 23:257-270.
18. Miller G.L. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal. Chem. 1959, 31:426-428.
19. Altschul S.F., et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25:3389-3402.
20. Zhou C., et al. Optimized expression of an acid xylanase from Aspergillus usamii in Pichia pastoris and its biochemical characterization. World J. Microbiol. Biotechnol. 2008, 24:1393-1401.
21. Ohashi R., et al. A mini-scale mass production and separation system for secretory heterologous proteins by perfusion culture of recombinant Pichia pastoris using a shaken ceramic membrane flask. J. Biosci. Bioeng. 1999, 87:655-660.
22. Villatte F., et al. Expression level of heterologous proteins in Pichia pastoris is influenced by flask design. Appl. Microbiol. Biotechnol. 2000, 55:463-465.
23. Wakiyama M., et al. Purification and properties of family-10 endo-1,4-b-xylanase from Penicillium citrinum and structural organization of encoding gene. J. Biosci. Bioeng. 2008, 105:367-374.
24. Gaffney M., et al. Purification and characterisation of a xylanase from Thermomyces lanuginosus and its functional expression by Pichia pastoris. Enzyme Microbiol. Technol. 2009, 45:348-354.
25. Deng P., et al. Cloning of a gene encoding an acidophilic endo-β-1,4-xylanase obtained from Aspergillus niger CGMCC1067 and constitutive expression in Pichia pastoris. Enzyme Microbiol. Technol. 2006, 39:1096-1102.
26. Ruanglek V., et al. Cloning, expression, characterisation, and high cell-density production of recombinant endo-1,4-β-xylanase from Aspergillus niger in Pichia pastoris. Enzyme Microbiol. Technol. 2007, 41:19-25.