Cross-Complementation Study of the Flagellar Type III Export Apparatus Membrane Protein FlhB

PLoS ONE

Volumn 7, Issue 8, 2012, Pages

  Barker, Clive S ^a   Samatey, Fadel A ^a  

^a OKINAWA INSTITUTE OF SCIENCE AND TECHNOLOGY GRADUATE UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

4 HYDROXY 3 METHYLBUT 2 EN 1 YL DIPHOSPHATE SYNTHASE; 4 HYDROXYBENZOATE OCTAPRENYLTRANSFERASE; AQUISALFLHB PROTEIN; BACTERIAL ENZYME; CHIMERIC PROTEIN; FLHA PROTEIN; FLHB PROTEIN; MEMBRANE PROTEIN; UBIQUINONE MENAQUINONE BIOSYNTHESIS METHYLTRANSFERASE; UNCLASSIFIED DRUG;

AQUIFEX AEOLICUS; ARTICLE; ASSAY; BACILLUS SUBTILIS; BACTERIAL FLAGELLUM; BACTERIAL GENOME; BACTERIAL STRAIN; CELL MOTILITY; CONTROLLED STUDY; CROSS COMPLEMENTATION ASSAY; GAIN OF FUNCTION MUTATION; LOSS OF FUNCTION MUTATION; NONHUMAN; PROTEIN DOMAIN; PROTEIN EXPRESSION; RESPIRATORY CHAIN; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SALMONELLA;

BACTERIA; BACTERIAL PROTEINS; BLOTTING, WESTERN; FLAGELLA; GENES, BACTERIAL; GENETIC COMPLEMENTATION TEST; MEMBRANE PROTEINS; MOVEMENT; PARABENS; PROTEIN TRANSPORT; RECOMBINANT PROTEINS; SALMONELLA; SUPPRESSION, GENETIC; UBIQUINONE;

AQUIFEX AEOLICUS; BACTERIA (MICROORGANISMS); EUKARYOTA; NEGIBACTERIA; SALMONELLA;

EID: 84865596112     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0044030     Document Type: Article

References (50)

1. Macnab RM, (2003) How bacteria assemble flagella. Annu Rev Microbiol 57: 77-100.
2. Chevance FFV, Hughes KT, (2008) Coordinating assembly of a bacterial macromolecular machine. Nature Rev Microbiol 6: 455-465.
3. Macnab RM, (2004) Type III flagellar protein export and flagellar assembly. Biochim Biophys Acta 1694: 207-217.
4. Cornelis GR, (2006) The type III secretion injectisome. Nature Rev Microbiol 4: 811-825.
5. Galán JE, Wolf-Watz H, (2006) Protein delivery into eukaryotic cells by type III secretion machines. Nature. 444: 567-573.
6. Minamino T, Namba K, (2008) Distinct roles of the FliI ATPase and proton motive force in bacterial flagellar protein export. Nature 451: 485-488.
7. Paul K, Erhardt M, Hirano T, Blair DF, Hughes KT, (2008) Energy source of flagellar type III secretion. Nature 451: 489-492.
8. Blocker A, Komoriya K, Aizawa SI, (2003) Type III secretion systems and bacterial flagella: insights into their function from structural similarities. Proc Natl Acad Sci U S A 100: 3027-3030.
9. Barker CS, Meshcheryakova IV, Kostyukova AS, Samatey FA, (2010) FliO regulation of FliP in the formation of the Salmonella enterica flagellum. PLoS Genet 6: e1001143 doi:10.1371/journal.pgen.1001143.
10. Minamino T, Imada K, Namba K, (2008) Mechanisms of type III protein export for bacterial flagellar assembly. Mol BioSyst 4: 1105-1115.
11. Bange G, Kümmerer N, Engel C, Bozkurt G, Wild K, et al. (2010) FlhA provides the adaptor for coordinated delivery of late flagella building blocks to the type III secretion system. Proc Natl Acad Sci U S A 107: 11295-11300.
12. Minamino T, Macnab RM, (2000) Interactions among components of the Salmonella flagellar export apparatus and its substrates. Mol Microbiol 35: 1052-1064.
13. Minamino T, Shimada M, Okabe M, Saijo-Hamano Y, Imada K, et al. (2010) Role of the C-terminal cytoplasmic domain of FlhA in bacterial flagellar type III protein export. J Bacteriol 192: 1929-1936.
14. Minamino T, Morimoto YV, Hara N, Namba K (2011) An energy transduction mechanism used in bacterial flagellar type III protein export. Nature Commun 2: doi: 10.1038/ncomms1488.
15. Fraser GM, Hirano T, Ferris HU, Devgan LL, Kihara M,et al. 2003. Substrate specificity of type III flagellar protein export in Salmonella is controlled by subdomain interactions in FlhB. Mol. Microbiol. 48: 1043-1057.
16. Morris DP, Roush ED, Thompson JW, Moseley MA, Murphy JW, et al. (2010) Kinetic characterization of Salmonella FliK-FlhB interactions demonstrates complexity of the type III secretion substrate-specificity switch. Biochemistry 49: 6386-6393.
17. Erhardt M, Hirano T, Su Y, Paul K, Wee DH, et al. (2010) The role of the FliK molecular ruler in hook-length control in Salmonella enterica. Mol Microbiol 75: 1272-1284.
18. Garza AG, Harris-Haller LW, Stoebner RA, Manson MD, (1995) Motility protein interactions in the bacterial flagellar motor. Proc Natl Acad Sci U S A 92: 1970-1974.
19. Zhou J, Lloyd SA, Blair DF, (1998) Electrostatic interactions between rotor and stator in the bacterial flagellar motor. Proc Natl Acad Sci U S A 95: 6436-6441.
20. Yakushi T, Yang J, Fukuoka H, Homma M, Blair DF, (2006) Roles of charged residues of rotor and stator in flagellar rotation: Comparative study using H+-driven and Na+-driven motors in Escherichia coli. J Bacteriol. 188: 1466-1472.
21. Kihara M, Minamino T, Yamaguchi S, Macnab RM, (2001) Intergenic suppression between the flagellar MS ring protein FliF of Salmonella and FlhA, a membrane component of its export apparatus. J Bacteriol 183: 1655-1662.
22. Ginocchio CC, Galán JE, (1995) Functional conservation among members of the Salmonella typhimurium InvA family of proteins. Infect Immun 63: 729-732.
23. Deckert G, Warren PV, Gaasterland T, Young WG, Lenox AL, et al. (1998) The complete genome of the hyperthermophilic bacterium Aquifex aeolicus. Nature 392: 353-358.
24. Meganathan R, Kwon O (2009) Biosynthesis of menaquinone (vitamin K2) and ubiquinone (coenzyme Q). In: Böck A, Curtiss III R, Kaper JB, Karp PD, Neidhardt FC, Nyström T, Slauch JM, Squires CL, Ussery D, editors. EcoSal-Escherichia coli and Salmonella: Cellular and Molecular Biology. Washington, D.C.: ASM Press, http://www.ecosal.org. Module 3.6.3.3.
25. Altincicek B, Kollas AK, Sanderbrand S, Wiesner J, Hintz M, et al. (2001) GcpE is involved in the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli. J Bacteriol 183: 2411-2416.
26. Young IG, Leppik RA, Hamilton JA, Gibson F, (1972) Biochemical and genetic studies on ubiquinone biosynthesis in Escherichia coli K-12: 4-Hydroxybenzoate octaprenyltransferase. J Bacteriol 110: 18-25.
27. Hara N, Namba K, Minamino T, (2011) Genetic characterization of conserved charged residues in the bacterial flagellar type III export protein FlhA. PLoS One 6: e22417 doi:10.1371/journal.pone.0022417.
28. Zhu K, González-Pedrajo B, Macnab RM, (2002) Interactions among membrane and soluble components of the flagellar export apparatus of Salmonella. Biochemistry 41: 9516-9524.
29. Bar Tana J, Howlett BJ, Koshland Jr DE, (1977) Flagellar formation in Escherichia coli electron transport mutants. J Bacteriol 130: 787-792.
30. Hertz R, Bar-Tana J, (1977) Anaerobic electron transport in anaerobic flagellum formation in Escherichia coli. J Bacteriol 132: 1034-1035.
31. Skinner MM, Puvathingal JM, Walter RL, Friedman AM, (2000) Crystal structure of protein isoaspartyl methyltransferase: A catalyst for protein repair. Structure 8: 1189-1201.
32. Singh S, McCoy JG, Zhang C, Bingman CA, Phillips Jr GN, et al. (2008) Structure and mechanism of the rebeccamycin sugar 4′-O-methyltransferase RebM. J Biol Chem 283: 22628-22636.
33. Lee M, Gräwert T, Quitterer F, Rohdich F, Eppinger J, et al. (2010) Biosynthesis of isoprenoids: crystal structure of the [4Fe-4S] cluster protein IspG. J Mol Biol 404: 600-610.
34. Rekittke I, Nonaka T, Wiesner J, Demmer U, Warkentin E, et al. (2011) Structure of the E-1-hydroxy-2-methyl-but-2-enyl-4-diphosphate synthase (GcpE) from Thermus thermophilus. FEBS Lett 585: 447-451.
35. Datsenko KA, Wanner BL, (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 97: 6640-6645.
36. Jiang W, Metcalf WW, Lee KS, Wanner BL, (1995) Molecular cloning, mapping, and regulation of Pho regulon genes for phosphonate breakdown by the phosphonatase pathway of Salmonella typhimurium LT2. J Bacteriol 177: 6411-6421.
37. Cherepanov PP, Wackernagel W, (1995) Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. Gene 158: 9-14.
38. Yamaguchi S, Fujita H, Sugata K, Taira T, Iino T, (1984) Genetic analysis of H2, the structural gene for phase-2 flagellin in Salmonella. J Gen Microbiol 130: 255-265.
39. Heckman KL, Pease LR, (2007) Gene splicing and mutagenesis by PCR-driven overlap extension. Nature Protoc 2: 924-932.
40. Ohnishi K, Fan F, Schoenhals GJ, Kihara M, Macnab RM, (1997) The FliO, FliP, FliQ, and FliR proteins of Salmonella typhimurium: putative components for flagellar assembly. J Bacteriol 179: 6092-6099.
41. Minamino T, Macnab RM, (2000) Domain structure of Salmonella FlhB, a flagellar export component responsible for substrate specificity switching. J Bacteriol 182: 4906-4914.
42. Ryu J, Hartin RJ, (1990) Quick transformation in Salmonella typhimurium LT2. Biotechniques. 8: 43-45.
43. Toker AS, Kihara M, Macnab RM, (1996) Deletion analysis of the FliM flagellar switch protein of Salmonella typhimurium. J Bacteriol 178: 7069-7079.
44. Li H, Ruan J, Durbin R, (2008) Mapping short DNA sequencing reads and calling variants using mapping quality scores. Genome Res 18: 1851-1858.
45. Qi J, Zhao F, Buboltz A, Schuster SC, (2010) inGAP: an integrated next-generation genome analysis pipeline. Bioinformatics 26: 127-129.
46. Bekker M, Kramer G, Hartog AF, Wagner MJ, de Koster CG, et al. (2007) Changes in the redox state and composition of the quinone pool of Escherichia coli during aerobic batch-culture growth. Microbiology. 153: 1974-1980.
47. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, et al. (2007) ClustalW and ClustalX version 2.0. Bioinformatics 23: 2947-2948.
48. Käll L, Krogh A, Sonnhammer EL, (2004) A combined transmembrane topology and signal peptide prediction method. J. Mol. Biol. 338: 1027-1036.
49. Käll L, Krogh A, Sonnhammer EL, (2005) An HMM posterior decoder for sequence feature prediction that includes homology information. Bioinformatics 21 (Suppl 1): i251-i257.
50. Cole C, Barber JD, Barton GJ, (2008) The Jpred 3 secondary structure prediction server. Nucleic Acids Res 36: W197-W021.