Evaluation of plasmid and genomic DNA calibrants used for the quantification of genetically modified organisms

Analytical and Bioanalytical Chemistry

Volumn 404, Issue 1, 2012, Pages 29-42

  Caprioara Buda, M ^a   Meyer, W ^a   Jeynov, B ^a   Corbisier, P ^a   Trapmann, S ^a   Emons, H ^a  

^a JOINT RESEARCH CENTRE   (Italy)

Author keywords

Certified reference material; DNA calibrant; DNA copy number ratio; Genetically modified organism; Plasmid; Real time PCR

Indexed keywords

CALIBRANTS; CERTIFIED REFERENCE MATERIALS; COPY NUMBER; GENETICALLY MODIFIED ORGANISMS; PLASMID; REAL-TIME PCR;

BIOLOGY; BIOMOLECULES; BIOSENSORS; CALIBRATION; DNA; EFFICIENCY; GENES; TISSUE; UNITS OF MEASUREMENT;

POLYMERASE CHAIN REACTION;

PLANT DNA;

ARTICLE; CALIBRATION; EVALUATION; GENE DOSAGE; GENETICS; GENOMICS; MAIZE; METHODOLOGY; PLASMID; STANDARD; TRANSGENIC PLANT;

CALIBRATION; DNA, PLANT; GENE DOSAGE; GENOMICS; PLANTS, GENETICALLY MODIFIED; PLASMIDS; ZEA MAYS;

ZEA MAYS;

EID: 84865585278     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-012-6104-6     Document Type: Article

References (37)

1. Regulation (EC) No 1830/2003 of the European Parliament and of the Council of 22 September 2003 concerning the traceability and labelling of genetically modified organisms and the traceability of food and feed products produced from genetically modified organisms and amending Directive 2001/18/EC (2003). Official Journal of the European Union L:24-28
2. Commission Recommendation of 4 October 2004 on technical guidance for sampling and detection of genetically modified organisms and material produced from genetically modified organisms as or in products in the context of Regulation (EC) No 1830/2003 (2004). Official Journal of the European Union L:18-26
3. Burns M, Corbisier P, Wiseman G, Valdivia H, McDonald P, Bowler P, Ohara K, Schimmel H, Charels D, Damant A (2006) Comparison of plasmid and genomic DNA calibrants for the quantification of genetically modified ingredients. Eur Food Res Technol 224:249-258 (Pubitemid 44770948)
4. Debode F, Marien A, Janssen E, Berben G (2010) Design of multiplex calibrant plasmids, their use in GMO detection and the limit of their applicability for quantitative purposes owing to competition effects. Anal Bioanal Chem 396:2151-2164
5. Taverniers I, Van Bockstaele E, De Loose M (2001) Use of cloned DNA fragments as reference materials for event specific quantification of genetically modified organisms (GMOs). Mededelingen (Rijksuniversiteit te Gent 66:469-472
6. Taverniers I, Windels P, Van Bockstaele E, De Loose M (2001) Use of cloned DNA fragments for event-specific quantification of genetically modified organisms in pure and mixed food produc. Eur Food Res Technol 213:417-424 (Pubitemid 33808458)
7. Taverniers I, Van Bockstaele E, De Loose M (2004) Cloned plasmid DNA fragments as calibrators for controlling GMOs: different real-time duplex quantitative PCR methods. Anal Bioanal Chem 378:1198-1207 (Pubitemid 40877715)
8. Lievens A, Bellocchi G, De Bernardi D, Moens W, Savini C, Mazzara M, Van den Eede G, Van den Bulcke M (2010) Use of pJANUS-02-001 as a calibrator plasmid for Roundup Ready soybean event GTS-40-3-2 detection: an interlaboratory trial assessment. Anal Bioanal Chem 396:2165-2173
9. Yang L, Pan A, Zhang K, Guo J, Yin C, Chen J, Huang C, Zhang D (2005) Identification and quantification of three genetically modified insect resistant cotton lines using conventional and Taq- Man real-time polymerase chain reaction methods. J Agric Food Chem 53:6222-6229 (Pubitemid 41169364)
10. Zhang H, Yang L, Guo J, Li X, Jiang L, Zhang D (2008) Development of one novel multiple-target plasmid for duplex quantitative PCR analysis of roundup ready soybean. J Agric Food Chem 56:5514-5520
11. Taverniers I, Windels P, Vaitilingom M, Milcamps A, Van Bockstaele E, Van den Eede G, De Loose M (2005) Event-specific plasmid standards and real-time PCR methods for transgenic Bt11, Bt176, and GA21 maize and transgenic GT73 canola. J Agric Food Chem 53:3041-3052 (Pubitemid 40525267)
12. Charels D, Broeders S, Corbisier P, Trapmann S, Schimmel H, Emons H (2007) Toward metrological traceability for DNA fragment ratios in GM quantification. 3. Suitability of DNA calibrants studied with a MON 810 corn model. J Agric Food Chem 55:3268-3274 (Pubitemid 46806535)
13. de Andrade E, Jeynov B, Corbisier P, Kortekaas AM, Trapmann S, Vincent S, Emons H (2011) Certification of a Maize NK603 Reference Material for its DNA Copy Number Ratio. Certified Reference Material ERM®-BF415e. ISBN 978-92-79-19074-2. EUR 24699 EN. Luxembourg: Publications Office of the European Union
14. Meyer W, de Andrade E, Broeders S, Charels D, Corbisier P, Merveillie A, Trapmann S, Emons H (2011) Certification of a Soya 356043 Reference Material for its DNA Copy Number Ratio. Certified Reference Material ERM®-BF425c. ISBN 978-92-79-19070-4. EUR 24695 EN. Luxembourg: Publications Office of the European Union
15. Caprioara-Buda M, Trapmann S, Gancberg D, Corbisier P,Merveillie A, Contreras Lopez MC, Emons H (2011) Certification of Maize 98140 Reference Material ERM®-BF427c. ISBN 978-92-79-19279-1. Luxembourg: Publications Office of the European Union
16. Trapmann S, Conneely P, Corbisier P, Gancberg D, Gioria S, Van Nyen M, Schimmel H, Emons H (2005) The Certification of Reference Materials of Dry-Mixed Maize Powder with different Mass Fractions of NK603 Maize Certified Reference Materials ERM®-BF415 (ERM®-BF415-a/ERM®-BF415-b/ERM®-BF415- c/ERM®-BF415-d/ERM®-BF415-e/ERM®-BF415-f). ISBN 92-894-8063-7. EUR 21270 EN. Luxembourg: Office for Official Publications of the European Communities
17. Gancberg D, Conneely P, Contreras Lopez M, Corbisier P, Gioria S, Van Nyen M, Vincent S, Trapmann (2007) Certification of Reference Materials of Soya Powder with different Mass Fractions of 356043 Soya, ERM®-BF425 (ERM®-BF425a, ERM®-BF425b, ERM®-BF425c, ERM®-BF425d). ISBN 978-92-79-07464-6. EUR 23029 EN. Luxembourg: Office for Official Publications of the European Communities
18. Gancberg D, Corbisier P, de Andrade Silva E, Mazoua S, Merveillie A, Tumba M-F, Trapmann S (2009) Certification of reference materials of maize seed powder with different mass fractions of genetically modified 98140 maize. Certified Reference Material ERM®- BF427 (ERM®-BF427a, ERM®-BF427b, ERM®-BF427c, ERM®- BF427d). ISBN 978-92-79-10174-8. EUR 23537 EN. Luxembourg: Office for Official Publications of the European Communities
19. Jeynov B, de Andrade E, Broothaerts W, Corbisier P, Mazoua S, Merveillie A, Trapmann S, Emons H (2011) Certification of Plasmid DNA Containing NK603 Maize DNA Fragments. Certified Reference Material ERM®-AD415. ISBN 978-92-79-19071-1. EUR 24696 EN. Luxembourg: Publications Office of the European Union
20. Corbisier P, Broeders S, Meeus N, Mazoua S, Meyer W, Trapmann S, Emons H (2011) Certification of Plasmid DNA Containing 356043 Soya DNA fragments. Certified Reference Material ERM®-AD425. ISBN 978-92-79-18993-7. EUR 24687 EN. Luxembourg: Publications Office of the European Union
21. Caprioara-Buda M, Corbisier P, Gancberg D, Broeders S, Trapmann S, Mazoua S, Matayron G, Emons H (2011) Certification of Plasmid DNA Containing 98140 Maize DNA Fragments. Certified Reference Material ERM®-AD427. ISBN 978-92-79-19277-7. EUR 24722 EN. Luxembourg: Publications Office of the European Union
22. Broothaerts W, Corbisier P, Schimmel H, Trapmann S, Vincent S, Emons H (2008) A single nucleotide polymorphism (SNP839) in the adh1 reference gene affects the quantitation of genetically modified maize (Zea mays L.). J Agric Food Chem 56:8825-8831
23. Event-Specific Method for the Quantitation of Maize Line NK603 Using Real-Time PCR - Validation Report and Protocol (2005) EU-RL GMFF. http://gmo-crl.jrc.ec.europa.eu/summaries/NK603report-mm.pdf. Accessed April 2010
24. Event-specific Method for the Quantification of Maize 98140 Using Real-time PCR - Validation Report and Protocol (2011) EU-RL GMFF. http://gmo-crl.jrc.ec.europa.eu/summaries/DP- 098140-6-validated-Method.pdf. Accessed January 2011
25. Event-specific Method for the Quantification of Soybean Event DP- 356043-5 Using Real-time PCR - Validation Report and Protocol (2009) EU-RL GMFF. http://gmo-crl.jrc.ec.europa.eu/summaries/DP- 356043-validated-Method- correctedversion1.pdf. Accessed April 2010
26. Edwards K, Johnstone C, Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19:1349
27. Arumuganathan K, Earle ED, Nuclear DNA (1991) Content of some important plant species. Plant Mol Biol Rep 9:208-218
28. Zhang D, Corlet A, Fouilloux S (2008) Impact of genetic structures on haploid genome-based quantification of genetically modified DNA: theoretical considerations, experimental data in MON 810 maize kernels (Zea mays L.) and some practical applications. Transgenic Res 17:393-402 (Pubitemid 351579733)
29. Charels D, Broeders S, Corbisier P, Trapmann S, Schimmel H, Linsinger T, Emons H (2007) Toward metrological traceability for DNA fragment ratios in GM quantification. 2. Systematic study of parameters influencing the quantitative determination of MON 810 corn by real-time PCR. J Agric Food Chem 55:3258-3267 (Pubitemid 46806534)
30. Definition of Minimum Performance Requirements for Analytical Methods of GMO Testing (2008). http://gmo-crl.jrc.ec.europa.eu/doc/Min-Perf-Requirements- Analytical-methods.pdf. Accessed September 2009
31. Schwarze J (ed.) (2009) Grundlagen der Statistik 2: Wahrscheinlichkeitsrechnung und induktive Statistik. Verlag Neue Wirtschafts- Briefe, GmbH & Co. KG, Herne 1986
32. Peano C, Samson MC, Palmieri L, Gulli M, Marmiroli N (2004) Qualitative and quantitative evaluation of the genomic DNA extracted from GMO and non-GMO foodstuffs with four different extraction methods. J Agric Food Chem 52:6962-6968
33. Smith DS, Maxwell PW (2005) Use of quantitative PCR to evaluate several methods for extracting DNA from corn Xour and cornstarch. Food Control 18:236-242
34. European Union Reference Laboratory for GM Food & Feed official website. http://gmo-crl.jrc.ec.europa.eu/statusofdoss.htm
35. Cankar K, Stebih D, Dreo T, Zel J, Gruden K (2006) Critical points of DNA quantification by real-time PCR - effects of DNA extraction method and sample matrix on quantification of genetically modified organisms. BMC Biotechnol 6:1-15
36. Holst-Jensen A, De Loose M, Van den Eede G (2006) Coherence between legal requirements and approaches for detection of genetically modified organisms (GMOs) and their derived products. J Agric Food Chem 54:2799-2809
37. ENGL explanatory document on the use of "Percentage of GM-DNA copy numbers in relation to target taxon specific DNA copy numbers calculated in terms of haploid genomes" as a general unit to express the percentage of GMOs (2007). http://engl.jrc.ec.europa.eu/docs/HGE-release.pdf. Accessed in September 2009