Development of one novel multiple-target plasmid for duplex quantitative PCR analysis of roundup ready soybean

Journal of Agricultural and Food Chemistry

Volumn 56, Issue 14, 2008, Pages 5514-5520

  Zhang, Haibo ^a   Yang, Litao ^a   Guo, Jinchao ^a   Li, Xiang ^a   Jiang, Lingxi ^a   Zhang, Dabing ^a  

^a SHANGHAI JIAO TONG UNIVERSITY   (China)

Author keywords

Duplex PCR; Genetically modified organisms; Plasmid with multiple targets; Roundup ready soybean

Indexed keywords

DRUG DERIVATIVE; GLYCINE; GLYPHOSATE; HERBICIDE; PLANT DNA; PLANT LECTIN; SOYBEAN LECTIN; SOYBEAN PROTEIN;

ARTICLE; CLASSIFICATION; FOOD PACKAGING; GENETICS; METHODOLOGY; PLASMID; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOYBEAN; TRANSGENIC PLANT;

DNA, PLANT; FOOD LABELING; GLYCINE; HERBICIDES; PLANT LECTINS; PLANTS, GENETICALLY MODIFIED; PLASMIDS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOYBEAN PROTEINS; SOYBEANS;

GLYCINE MAX;

EID: 49049091761     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf800033k     Document Type: Article

References (26)

1. James, C. Executive summary of global status of commercialized biotech/GM crops. ISAAA Briefs 2007, No. 37.
2. Authority of Agricultural Genetically Modified Organisms Imported As Raw and Processed Materials in China. http://www.stee.agri.gov.cn/biosafety/spxx/ t20051107_488946.htm.
3. European Commission Regulation (EC) 1829/2003 and 1830/2003. Off. J. Europe Communities 2003L 268, 1-28.
4. Notification 2007-43;Ministry of Agriculture and Forestry of Korea: Seoul, Korea,2007.
5. Notification 1775, Food and Marketing Bureau, Ministry of Agriculture, Forestry and Fisheries of Japan,: Tokyo, Japan, 2000.
6. Holst-Jensen, A.; Røning, S. B.; Løseth, A.; Berdal, K. G. PCR technology for screening and quantification of genetically modified organisms (GMOs). Anal. Bioanal. Chem. 2003, 375, 985-993.
7. Huang, C. C.; Pan, T. M. Event-specific real-time detection and quantification of genetically modified Roundup Ready soybean. J. Agric. Food Chem. 2005, 53, 3833-3839.
8. Yang, L. T.; Guo, J. C.; Pan, A. H.; Zhang, H. B.; Zhang, K. W.; Wang, Z. M.; Zhang, D. B. Event-specific quantitative detection of nine genetically modified maizes using one novel standard reference molecule. J. Agric. Food Chem. 2007, 55, 15-24.
9. Windels, P.; Taverniers, I.; Depicker, A.; Van Bockstaele, E.; De Loose, M. Characterization of the Roundup Ready soybean insert. Eur. Food Res. Technol. 2001, 213, 107-112.
10. Berdal, K. G.; Holst-Jensen, A. Roundup Ready soybean event specific real-time quantitative PCR assay and estimation of the practical detection and quantification limits in GMO analyses. Eur. Food Res. Technol. 2001, 213, 432-438.
11. Taverniers, I.; Windeis, P.; van Bockstaele, E.; De Loose, M. Use of cloned DNA fragments for event-specific quantification of genetically modified organisms in pure and mixed food products. Eur. Food Res. Technol. 2001, 213, 417-424.
12. Andersen, C. B.; Holst-Jensen, A.; Berdal, K. G.; Thorstensen, T.; Tengs, T. Equal performance of TaqMan, MGB, molecular beacon, and SYBR green-based detection assays in detection and quantification of Roundup Ready soybean. J. Agric. Food Chem. 2006, 54, 9658-9663.
13. Burns, M.; Corbisier, P.; Wiseman, G.; Valdivia, H.; McDonald, P.; Bowler, P.; Ohara, K.; Schimmel, H.; Charels, D.; Damant, A.; Harris, N. Comparison of plasmid and genomic DNA calibrants for the quantification of genetically modified ingredients. Eur Food Res Technol. 2006, 224, 249-258.
14. Alary, R.; Serin, A.; Maury, D.; Jouira, H. B.; Sirven, J.; Gautier, M.; Joudrier, P. Comparison of simplex and duplex real-time PCR for the quantification of GMO in maize and soybean. Food Control 2002, 13, 235-244.
15. Taverniers, I.; Van Bockstaele, E.; De Loose, M. Cloned plasmid DNA fragments as calibrators for controlling GMOs: different real-time duplex quantitative PCR methods. Anal Bioanal Chem. 2004, 378, 1198-1207.
16. Weighardt, F.; Barbati, C.; Paoletti, C.; Querci, M.; Kay, S.; DeBeukeleer, M.; Van den Eede, G. Real-time polymerase chain reaction-based approach for quantification of the pat gene in the T25 Zea mays event. J AOAC Int. 2004, 87, 1342-1355.
17. Mattarucchi, E.; Weighardt, F.; Barbati, C.; Querci, M.; Van den Eede, G. Development and applications of real-time PCR standards for GMO quantification based on tandem-marker plasmids. Eur Food Res Technol. 2005, 221, 511-519.
18. Kuribara, H.; Shindo, Y.; Matsuoka, T.; Takubo, K.; Futo, S.; Aoki, N.; Hirao, T.; Akiyama, H.; Goda, Y.; Toyoda, M.; Hino, A. Novel reference molecules for quantitation of genetically modified maize and soybean. J AOAC Int. 2002, 85 (5), 1077-1089.
19. Yang, L. T.; Pan, A. H.; Zhang, K. W.; Yin, C. S.; Qian, B. J.; Chen, J. X.; Huang, C.; Zhang, D. B. Qualitative and quantitative PCR methods for event-specific detection of genetically modified cotton Mon1445 and Mon531. Transgenic Res. 2005, 14, 817-831.
20. Corbisier, P.; Trapmann, S.; Gancberg, D,.; Hannes, L.; Van Iwaarden, P.; Berben, G.; Schimmel, H,.; Emons, H. Quantitative determination of Roundup Ready soybean (Glycine max) extracted from highly processed flour. Eur. Food Res. Technol. 2001, 213, 417-424.
21. Yang, L. T.; Shen, H. F.; Pan, A. H.; Chen, J. X.; Huang, C.; Zhang, D. B. Screening and construct-specific detection methods of transgenic Huafan No 1 tomato by conventional and real-time PCR. J. Sci. Food Agric. 2005, 85 (13), 2159-2166.
22. Yoshimura, T.; Kuribara, H.; Matsuoka, T.; Kodama, T.; Iida, M.; Watanabe, T.; Akiyama, H.; Maitani, T.; Furui, S.; Hino, A. Applicability of quantification of genetically modified organisms to foods processed from maize and soy. J. Agric. Food Chem. 2005, 53, 2052-2059.
23. Sambrook, J.; Russell, D. W. Molecular Cloning: A Laboratory Manual (Three-Volume Set); Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 2001.
24. Burns, M.; Corbisier, P.; Wiseman, G.; Valdivia, H.; McDonald, P.; Bowler, P.; Ohara, K.; Schimmel, H.; Charels, D.; Damant, A.; Harris, N. Comparison of plasmid and genomic DNA calibrants for the quantification of genetically modified ingredients. Eur. Food Res. Technol. 2006, 224, 249-258.
25. Charels, D.; Breeders, S.; Corbisier, P.; Trapmann, S.; Schimmel, H.; Emons, H. Toward metrological traceability for DNA fragment ratios in GM quantification. 3. Suitability of DNA calibrants studied with a MON corn. J. Agric. Food Chem. 2007, 55 (9), 3268-74.
26. Alary, R.; Serin, A.; Maury, D.; Jouira, H. B.; Sirven, J.; Gautier, M.; Joudrier, P. Comparison of simplex and duplex real-time PCR for the quantification of GMO in maize and soybean. Food Control 2002, 13, 235-244.