Molecular cloning and characterization of a novel β-glucosidase with high hydrolyzing ability for soybean isoflavone glycosides and glucose-tolerance from soil metagenomic library

Bioresource Technology

Volumn 123, Issue , 2012, Pages 15-22

  Li, Gang ^a   Jiang, Yang ^a   Fan, Xin Jiong ^a   Liu, Yu Huan ^a  

^a SUN YAT SEN UNIVERSITY   (China)

Author keywords

Glucosidase; Enzyme characterization; Glucose tolerance; Metagenomic library; Soybean isoflavone hydrolysis

Indexed keywords

ENZYME CHARACTERIZATION; GLUCOSE TOLERANCE; GLUCOSIDASE; ISOFLAVONES; METAGENOMIC LIBRARIES;

AMINO ACIDS; CLONING; ENZYMES; ESCHERICHIA COLI; FLAVONOIDS; GLUCOSE; HYDROLYSIS; METAL IONS; SOILS;

OILSEEDS;

BETA GLUCOSIDASE; DAIDZIN; GENISTEIN; GLYCOSIDE; RECOMBINANT ENZYME;

COLIFORM BACTERIUM; ENZYME ACTIVITY; GENE EXPRESSION; GENOMICS; GLUCOSE; HYDROLYSIS; MOLECULAR ANALYSIS; PH; RECOMBINATION; SOYBEAN; TEMPERATURE EFFECT; TOLERANCE;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVITY; ENZYME STABILITY; ESCHERICHIA COLI; GLUCOSE TOLERANCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; METAGENOMICS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SOIL; SOYBEAN; TEMPERATURE; THERMOSTABILITY;

AMINO ACID SEQUENCE; BETA-GLUCOSIDASE; BIOCATALYSIS; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; GENE LIBRARY; GLUCOSE; GLUCOSIDES; GLYCOSIDES; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; INDICATORS AND REAGENTS; IONS; ISOFLAVONES; KINETICS; METAGENOME; METALS; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SOIL MICROBIOLOGY; SOYBEANS; TEMPERATURE;

ESCHERICHIA COLI; GLYCINE MAX;

EID: 84865564716     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2012.07.083     Document Type: Article

References (35)

1. Amann R.I., Ludwig W., Schleifer K.H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 1995, 59:143-169.
2. Banik J.J., Brady S.F. Recent application of metagenomic approaches toward the discovery of antimicrobials and other bioactive small molecules. Curr. Opin. Microbiol. 2010, 13:603-609.
3. Bao L., Huang Q., Chang L., Sun Q., Zhou J., Lu H. Cloning and characterization of two β-glucosidase/xylosidase enzymes from yak rumen metagenome. Appl. Biochem. Biotechnol. 2012, 166:72-86.
4. Benoliel B., Pocas-Fonseca J.P., Araripe F., Torres G., de Moraes L.M.P. Expression of a glucose-tolerant β-glucosidase from Humicola grisea var. thermoidea in Saccharomyces cerevisiae. Appl. Biochem. Biotechnol. 2010, 160:2036-2044.
5. Chiou T.Y., Lin Y.H., Su N.W., Lee M.H. Beta-glucosidase isolated from soybean okara shows specificity toward glucosyl isoflavones. J. Agric. Food. Chem. 2010, 58:8872-8878.
6. Cotter A., Cashman K.D. Genistein appears to prevent early postmenopausal bone loss as effectively as hormone replacement therapy. Nat. Rev. 2003, 61:346-351.
7. Coughlan M.P. The properties of fungal and bacterial cellulases with comments on their production and application. Biotechnol. Genet. Eng. Rev. 1985, 3:39-109.
8. Curtis T.P., Sloan W.T. Exploring microbial diversity - a vast below. Science 2005, 309:1331-1333.
9. Dixon M. The determination of enzyme inhibitor constants. Biochem. J. 1953, 55:170-171.
10. Dotsenko G.S., Sinitsyna O.A., Hinz S.W.A., Wery J., Sinitsyn A.P. Characterization of a GH family 3β-glycioside hydrolase from Chrysosporium lucknowense and its application to the hydrolysis of β-glucan and xylan. Bioresour. Technol. 2012, 112:345-349.
11. Eberhart B., Cross D.F., Chase L.R. Beta-glucosidase system of Neurospora crass I. beta-glucosidase and cellulose activities of mutant and wild-type strains. J. Bacteriol. 1964, 87:761-770.
12. Fang Z., Fang W., Liu J., Hong Y., Peng H., Zhang X., Sun B., Xiao Y. Cloning and characterization of a β-glucosidase from marine microbial metagenome with excellent glucose tolerance. J. Microbiol. Biotechnol. 2010, 20:1351-1358.
13. Feng Y., Duan C.J., Liu L., Tang J.L., Feng J.X. Properties of a metagenome-derived β-glucosidase from the contents of rabbit cecum. Biosci., Biotechnol., Biochem. 2009, 73:1470-1473.
14. Goodman-Gruen D., Kritz-Silverstein D. Usual dietary isoflavone intake is associated with cardiovascular disease risk factors in postmenopausal women. J. Nutr. 2001, 131:1202-1206.
15. Gueguen Y., Chemardin P., Janbon G., Arnaud A., Galzy P. A very efficient β-glucosidase catalyst for the hydrolysis of flavor precursors of wines and fruit juices. J. Agric. Food. Chem. 1996, 44:2336-2340.
16. Handelsman J., Rondon M.R., Brady S.F., Clardy J., Goodman R.M. Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem. Biol. 1998, 5:R245-R249.
17. Horri K., Adachi T., Matsuda T., Tanaka T., Sahara H., Shibasaki S., Ogino C., Hata Y., Ueda M., Kondo A. Improvement of isoflavone aglycones production using beta-glucosidase secretory produced in recombinant Aspergillus oryzae. J. Mol. Catal. B: Enzym. 2009, 59:297-301.
18. Ismail B., Hayes K. Beta-glycosidase activity toward different glycosidic forms of isoflavones. J. Agric. Food. Chem. 2005, 53:4918-4924.
19. Izumi T., Piskula M.K., Osawa S., Obata A., Tobe K., Saito M., Kataoka S., Kubota Y., Kikuchi M. Soy isoflavone aglycones are absorbed faster and in higher amounts than their glucosides in humans. J. Nutr. 2000, 130:1695-1699.
20. Jiang C., Li S.X., Luo F.F., Jin K., Wang Q., Hao Z.Y., Wu L.L., Zhao G.C., Ma G.F., Shen P.H., Tang X.L., Wu B. Biochemical characterization of two novel-β-glucosidase genes by metagenome expression cloning. Bioresour. Technol. 2011, 102:3272-3278.
21. Karnchanatat A., Petsom A., Sangvanich P., Piaphukiew J., Whalley A.J., Reynolds C.D., Sihanonth P. Purification and biochemical characterization of an extracellular beta-glucosidase from the wood-decaying fungus Daldinia eschscholzii (Ehrenb.:Fr.) Rehm. FEMS Microbiol. Lett. 2007, 270:162-170.
22. Kim B.N., Yeom S.J., Kim Y.S., Oh D.K. Characterization of a β-glucosidase from Sulfolobus solfataricus for isoflavone glycosides. Biotechnol. Lett. 2012, 34:125-129.
23. Krisch J., Bencsik O., Papp T., Vágvölgyi C., TaKó M. Characterization of a β-glucosidase with transgalactosylation capacity from the zygomycete Rhizomucor miehei. Bioresour. Technol. 2012, 114:555-560.
24. Kuo L.C., Lee K.T. Cloning, expression, and characterization of two beta-glucosidases from isoflavone glycoside-hydrolyzing Bacillus subtilis natto. J. Agric. Food. Chem. 2008, 56:119-125.
25. Matsuura M., Obata A. β-Glucosidases from soybeans hydrolyze daidzin and genistin. J. Food Sci. 1993, 58:144-147.
26. Parry N.J., Beever D.E., Owen E., Vandenberghe I., Van Beeumen J., Bhat M.K. Biochemical characterization and mechanism of action of a thermostable beta-glucosidase purified from Thermoascus aurantiacus. Biochem. J. 2001, 353:117-127.
27. Ravindranath M.H., Muthugounder S., Presser N., Viswanathan S. Anticancer therapeutic potential of soy isoflavone, genistein. Adv. Exp. Med. Biol. 2004, 546:121-165.
28. Riou C., Salmon J., Vallierl M., Gunata Z., Barre P. Purification, characterization and substrate specificity of a novel highly glucose-tolerant β-glucosidase from Aspergillus oryzae. Appl. Environ. Microbiol. 1998, 64:3607-3614.
29. Saha B., Bothast R. Production, purification and characterization of a highly glucose-tolerant novel β-glucosidase from Candida peltata. Appl. Environ. Microbiol. 1996, 62:3165-3170.
30. Sonia K.G., Chadha B.S., Badhan A.K., Saini H.S., Bhat M.K. Identification of glucose tolerant acid active β-glucosidases from thermophilic and thermotolerant fungi. World J. Microbiol. Biotechnol. 2008, 24:599-604.
31. Uchima C.A., Tokuda G., Watanabe H., Kitamoto K., Arioka M. Heterologous expression in Pichia pastoris and characterization of an endogenous thermostable and high-glucose-tolerant β-glucosidase from the termite Nasutitermes takasagoensis. Appl. Environ. Microbiol. 2012, 78:4288-4293.
32. Xue Y., Yu J., Song X. Hydrolysis of soy isoflavone glycosides by recombinant beta-glucosidase from hyperthermophile Thermotoga maritime. J. Ind. Microbiol. Biotechnol. 2009, 36:1401-1408.
33. Yan Q.J., Hua C.W., Yang S.Q., Li Y.N., Jiang Z.Q. High level expression of extracellular secretion of a β-glycioside gene (PtBglu3) from Paecilomyces thermophila in Pichia pastoris. Protein Expression Purif. 2012, 84:64-72.
34. Yang S., Wang L., Yan Q., Jiang Z., Li L. Hydrolysis of soybean isoflavone glycosides by a thermostable beta-glucosidase from Paecilomyces thermophila. Food Chem. 2009, 115:1247-1252.
35. Yeom S.J., Kim B.N., Kim Y.S., Oh D.K. Hydrolysis of isoflavone glycosides by a thermostable β-glucosidase from Pyrococcus furiosus. J. Agric. Food. Chem. 2012, 60:1535-1541.