Development of a peptidase-resistant substrate for single-cell measurement of protein kinase b activation

Analytical Chemistry

Volumn 84, Issue 16, 2012, Pages 7195-7202

  Proctor, Angela ^a   Wang, Qunzhao ^a   Lawrence, David S ^a,b   Allbritton, Nancy L ^a,b,c  

^a University of North Carolina at Chapel Hill   (United States)

^b UNIVERSITY OF NORTH CAROLINA   (United States)

^c NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOSOLIC; ITERATIVE DESIGN; KINASE ACTIVITY; KINASE SUBSTRATES; LIFETIME IMPROVEMENT; LNCAP CELLS; LYSATES; NON-NATIVE; PEPTIDE FRAGMENTS; PROTEIN KINASE; PROTEIN KINASE B; SINGLE CELLS; SINGLE-CELL MEASUREMENTS; SUBSTRATE PEPTIDES;

AMINO ACIDS; CELLS; CYTOLOGY; DEGRADATION; ENZYMES; PHOSPHORYLATION; SUBSTRATES;

PEPTIDES;

ARGININE; OLIGOPEPTIDE; PEPTIDE HYDROLASE; PROTEIN KINASE B; PROTEIN KINASE INHIBITOR;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CHEMISTRY; CYTOSOL; DRUG ANTAGONISM; DRUG DESIGN; DRUG EFFECT; ENZYME ACTIVATION; HELA CELL; HUMAN; METABOLISM; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN DEGRADATION; SINGLE CELL ANALYSIS;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARGININE; CYTOSOL; DRUG DESIGN; ENZYME ACTIVATION; HELA CELLS; HUMANS; OLIGOPEPTIDES; PEPTIDE HYDROLASES; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN KINASE INHIBITORS; PROTEOLYSIS; PROTO-ONCOGENE PROTEINS C-AKT; SINGLE-CELL ANALYSIS;

EID: 84865448302     PISSN: 00032700     EISSN: 15206882     Source Type: Journal    
DOI: 10.1021/ac301489d     Document Type: Article

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