RNA interference-mediated gene knockdown within specific cell types

Plant Molecular Biology

Volumn 80, Issue 2, 2012, Pages 169-176

  Zhang, Changqing ^a,b   Galbraith, David W ^a  

^a UNIVERSITY OF ARIZONA   (United States)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

GFP; RNAi; Silencing signal

Indexed keywords

ARABIDOPSIS PROTEIN; DOUBLE STRANDED RNA; GREEN FLUORESCENT PROTEIN; HOMEODOMAIN PROTEIN; WUSCHEL PROTEIN, ARABIDOPSIS;

ARABIDOPSIS; ARTICLE; CONFOCAL MICROSCOPY; CYTOLOGY; GENE EXPRESSION REGULATION; GENE SILENCING; GENE VECTOR; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLANT ROOT; RNA INTERFERENCE; TRANSGENIC PLANT;

ARABIDOPSIS; ARABIDOPSIS PROTEINS; GENE EXPRESSION REGULATION, PLANT; GENE KNOCKDOWN TECHNIQUES; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HOMEODOMAIN PROTEINS; MICROSCOPY, CONFOCAL; MOLECULAR SEQUENCE DATA; PLANT ROOTS; PLANTS, GENETICALLY MODIFIED; RNA INTERFERENCE; RNA, DOUBLE-STRANDED;

ARABIDOPSIS;

EID: 84865413438     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11103-012-9937-7     Document Type: Article

References (30)

1. Birnbaum K, Shasha DE, Wang JY, Jung JW, Lambert GM, Galbraith DW, Benfey PN (2003) A gene expression map of the Arabidopsis root. Science 302(5652): 1956-1960. doi: 10. 1126/science. 1090022.
2. Brosnan CA, Voinnet O (2011) Cell-to-cell and long-distance siRNA movement in plants: mechanisms and biological implications. Curr Opin Plant Biol. doi: 10. 1016/j. pbi. 2011. 07. 011.
3. Byzova M, Verduyn C, De Brouwer D, De Block M (2004) Transforming petals into sepaloid organs in Arabidopsis and oilseed rape: implementation of the hairpin RNA-mediated gene silencing technology in an organ-specific manner. Planta 218(3): 379-387. doi: 10. 1007/s00425-003-1117-1.
4. Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16(6): 735-743.
5. Di Laurenzio L, Wysocka-Diller J, Malamy JE, Pysh L, Helariutta Y, Freshour G, Hahn MG, Feldmann KA, Benfey PN (1996) The SCARECROW gene regulates an asymmetric cell division that is essential for generating the radial organization of the Arabidopsis root. Cell 86(3): 423-433.
6. Dolan L, Janmaat K, Willemsen V, Linstead P, Poethig S, Roberts K, Scheres B (1993) Cellular-organization of the Arabidopsis-thaliana root. Development 119(1): 71-84.
7. Dunoyer P, Schott G, Himber C, Meyer D, Takeda A, Carrington JC, Voinnet O (2010) Small RNA duplexes function as mobile silencing signals between plant cells. Science 328(5980): 912-916. doi: 10. 1126/science. 1185880.
8. Finnegan EJ, Peacock WJ, Dennis ES (1996) Reduced DNA methylation in Arabidopsis thaliana results in abnormal plant development. Proc Natl Acad Sci USA 93(16): 8449-8454.
9. Haecker A, Gross-Hardt R, Geiges B, Sarkar A, Breuninger H, Herrmann M, Laux T (2004) Expression dynamics of WOX genes mark cell fate decisions during early embryonic patterning in Arabidopsis thaliana. Development 131(3): 657-668. doi: 10. 1242/dev. 00963.
10. Hamilton AJ, Baulcombe DC (1999) A species of small antisense RNA in posttranscriptional gene silencing in plants. Science 286(5441): 950-952.
11. Hammond SM, Bernstein E, Beach D, Hannon GJ (2000) An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature 404(6775): 293-296. doi: 10. 1038/35005107.
12. Heidstra R, Welch D, Scheres B (2004) Mosaic analyses using marked activation and deletion clones dissect Arabidopsis SCARECROW action in asymmetric cell division. Gene Dev 18(16): 1964-1969. doi: 10. 1101/Gad. 305504.
13. Helliwell CA, Waterhouse PM (2005) Constructs and methods for hairpin RNA-mediated gene silencing in plants. Method Enzymol 392: 24-35.
14. Himber C, Dunoyer P, Moissiard G, Ritzenthaler C, Voinnet O (2003) Transitivity-dependent and -independent cell-to-cell movement of RNA silencing. EMBO J 22(17): 4523-4533.
15. Islam S, Miyazaki T, Tanno F, Itoh K (2005) Dissection of gene function by RNA silencing. Plant Biotechnol 22: 4.
16. Limpens E, Ramos J, Franken C, Raz V, Compaan B, Franssen H, Bisseling T, Geurts R (2004) RNA interference in Agrobacterium rhizogenes-transformed roots of Arabidopsis and Medicago truncatula. J Exp Bot 55(399): 983-992. doi: 10. 1093/Jxb/Erh122.
17. Malamy JE, Benfey PN (1997) Organization and cell differentiation in lateral roots of Arabidopsis thaliana. Development 124(1): 33-44.
18. McGinnis K, Chandler V, Cone K, Kaeppler H, Kaeppler S, Kerschen A, Pikaard C, Richards E, Sidorenko L, Smith T, Springer N, Wulan T (2005) Transgene-induced RNA interference as a tool for plant functional genomics. Method Enzymol 392: 1-24.
19. Morel JB, Mourrain P, Beclin C, Vaucheret H (2000) DNA methylation and chromatin structure affect transcriptional and post-transcriptional transgene silencing in Arabidopsis. Curr Biol 10(24): 1591-1594.
20. Palauqui JC, Elmayan T, Pollien JM, Vaucheret H (1997) Systemic acquired silencing: transgene-specific post-transcriptional silencing is transmitted by grafting from silenced stocks to non-silenced scions. EMBO J 16(15): 4738-4745.
21. Ronemus MJ, Galbiati M, Ticknor C, Chen JC, Dellaporta SL (1996) Demethylation-induced developmental pleiotropy in Arabidopsis. Science 273(5275): 654-657.
22. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn, Cold Spring Harbor Laboratory Press.
23. Sunilkumar G, Campbell LM, Puckhaber L, Stipanovic RD, Rathore KS (2006) Engineering cottonseed for use in human nutrition by tissue-specific reduction of toxic gossypol. Proc Natl Acad Sci USA 103(48): 18054-18059. doi: 10. 1073/pnas. 0605389103.
24. Voinnet O, Vain P, Angell S, Baulcombe DC (1998) Systemic spread of sequence-specific transgene RNA degradation in plants is initiated by localized introduction of ectopic promoterless DNA. Cell 95(2): 177-187.
25. Waterhouse PM, Helliwell CA (2003) Exploring plant genomes by RNA-induced gene silencing. Nat Rev Genet 4(1): 29-38. doi: 10. 1038/Nrg982.
26. Wysocka-Diller JW, Helariutta Y, Fukaki H, Malamy JE, Benfey PN (2000) Molecular analysis of SCARECROW function reveals a radial patterning mechanism common to root and shoot. Development 127(3): 595-603.
27. Yoo BC, Kragler F, Varkonyi-Gasic E, Haywood V, Archer-Evans S, Lee YM, Lough TJ, Lucas WJ (2004) A systemic small RNA signaling system in plants. Plant Cell 16(8): 1979-2000. doi: 10. 1105/tpc. 104. 023614.
28. Zamore PD, Tuschl T, Sharp PA, Bartel DP (2000) RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell 101(1): 25-33.
29. Zhang C, Gong FC, Lambert GM, Galbraith DW (2005) Cell type-specific characterization of nuclear DNA contents within complex tissues and organs. Plant Methods 1(1): 7. doi: 10. 1186/1746-4811-1-7.
30. Zhang C, Barthelson RA, Lambert GM, Galbraith DW (2008) Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei. Plant Physiol 147(1): 30-40. doi: 10. 1104/pp. 107. 115246.