Demethylation-induced developmental pleiotropy in Arabidopsis

Science

Volumn 273, Issue 5275, 1996, Pages 654-657

  Ronemus, Michael J ^a   Galbiati, Massimo ^a   Ticknor, Christine ^a   Chen, Jychian ^b   Dellaporta, Stephen L ^a  

^a YALE UNIVERSITY   (United States)

^b ACADEMIA SINICA   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; COMPLEMENTARY RNA;

ARABIDOPSIS; DEMETHYLATION; DNA METHYLATION; GENE EXPRESSION; GENE SEQUENCE; PLANT GROWTH; PLEIOTROPY; PRIORITY JOURNAL; REVIEW; TRANSGENE; TRANSGENIC PLANT;

ARABIDOPSIS; ARABIDOPSIS THALIANA; EMBRYOPHYTA;

EID: 0029765771     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.273.5275.654     Document Type: Review

References (45)

1. Y. Gruenbaum, T. Naveh-Many, H. Cedar, A. Razin, Nature 292, 860 (1981).
2. F. F. Assaad, K. L. Tucker, E. R. Signer, Plant Mol. Biol. 22, 1067 (1993); C. Napoli, C. Lemieux, R. Jorgensen, Plant Cell 2, 279 (1990); J. Bender and G. R. Fink, Cell 83, 725 (1995); P. S. Chomet, S. Wessler, S. L. Dellaporta, EMBO J. 6, 295 (1987); T. P. Brutnell and S. L. Dellaporta, Genetics 138, 213 (1994); R. A. Martienssen and E. J. Richards, Curr. Opin. Genet. Dev. 5, 234 (1995); M. A. Matzke and A. J. M. Matzke, Plant Physiol. 107, 679 (1995).
3. F. F. Assaad, K. L. Tucker, E. R. Signer, Plant Mol. Biol. 22, 1067 (1993); C. Napoli, C. Lemieux, R. Jorgensen, Plant Cell 2, 279 (1990); J. Bender and G. R. Fink, Cell 83, 725 (1995); P. S. Chomet, S. Wessler, S. L. Dellaporta, EMBO J. 6, 295 (1987); T. P. Brutnell and S. L. Dellaporta, Genetics 138, 213 (1994); R. A. Martienssen and E. J. Richards, Curr. Opin. Genet. Dev. 5, 234 (1995); M. A. Matzke and A. J. M. Matzke, Plant Physiol. 107, 679 (1995).
4. F. F. Assaad, K. L. Tucker, E. R. Signer, Plant Mol. Biol. 22, 1067 (1993); C. Napoli, C. Lemieux, R. Jorgensen, Plant Cell 2, 279 (1990); J. Bender and G. R. Fink, Cell 83, 725 (1995); P. S. Chomet, S. Wessler, S. L. Dellaporta, EMBO J. 6, 295 (1987); T. P. Brutnell and S. L. Dellaporta, Genetics 138, 213 (1994); R. A. Martienssen and E. J. Richards, Curr. Opin. Genet. Dev. 5, 234 (1995); M. A. Matzke and A. J. M. Matzke, Plant Physiol. 107, 679 (1995).
5. F. F. Assaad, K. L. Tucker, E. R. Signer, Plant Mol. Biol. 22, 1067 (1993); C. Napoli, C. Lemieux, R. Jorgensen, Plant Cell 2, 279 (1990); J. Bender and G. R. Fink, Cell 83, 725 (1995); P. S. Chomet, S. Wessler, S. L. Dellaporta, EMBO J. 6, 295 (1987); T. P. Brutnell and S. L. Dellaporta, Genetics 138, 213 (1994); R. A. Martienssen and E. J. Richards, Curr. Opin. Genet. Dev. 5, 234 (1995); M. A. Matzke and A. J. M. Matzke, Plant Physiol. 107, 679 (1995).
6. F. F. Assaad, K. L. Tucker, E. R. Signer, Plant Mol. Biol. 22, 1067 (1993); C. Napoli, C. Lemieux, R. Jorgensen, Plant Cell 2, 279 (1990); J. Bender and G. R. Fink, Cell 83, 725 (1995); P. S. Chomet, S. Wessler, S. L. Dellaporta, EMBO J. 6, 295 (1987); T. P. Brutnell and S. L. Dellaporta, Genetics 138, 213 (1994); R. A. Martienssen and E. J. Richards, Curr. Opin. Genet. Dev. 5, 234 (1995); M. A. Matzke and A. J. M. Matzke, Plant Physiol. 107, 679 (1995).
7. F. F. Assaad, K. L. Tucker, E. R. Signer, Plant Mol. Biol. 22, 1067 (1993); C. Napoli, C. Lemieux, R. Jorgensen, Plant Cell 2, 279 (1990); J. Bender and G. R. Fink, Cell 83, 725 (1995); P. S. Chomet, S. Wessler, S. L. Dellaporta, EMBO J. 6, 295 (1987); T. P. Brutnell and S. L. Dellaporta, Genetics 138, 213 (1994); R. A. Martienssen and E. J. Richards, Curr. Opin. Genet. Dev. 5, 234 (1995); M. A. Matzke and A. J. M. Matzke, Plant Physiol. 107, 679 (1995).
8. F. F. Assaad, K. L. Tucker, E. R. Signer, Plant Mol. Biol. 22, 1067 (1993); C. Napoli, C. Lemieux, R. Jorgensen, Plant Cell 2, 279 (1990); J. Bender and G. R. Fink, Cell 83, 725 (1995); P. S. Chomet, S. Wessler, S. L. Dellaporta, EMBO J. 6, 295 (1987); T. P. Brutnell and S. L. Dellaporta, Genetics 138, 213 (1994); R. A. Martienssen and E. J. Richards, Curr. Opin. Genet. Dev. 5, 234 (1995); M. A. Matzke and A. J. M. Matzke, Plant Physiol. 107, 679 (1995).
9. A. Vongs, T. Kakutani, R. A. Martienssen, E. J. Richards, Science 260, 1926 (1993).
10. T. Kakutani, J. A. Jeddeloh, E. J. Richards, Nucleic Acids Res. 23, 130 (1995).
11. E. J. Finnegan and E. S. Dennis, ibid. 21, 2383 (1993).
12. The MET1 cDNA (B6-2) used in these experiment was cloned independently; it lacked 436 base pairs (bp) downstream of the deduced translation start site and differed from the published sequence by a thymine-to-cytosine substitution at position +2981.
13. The MET1 gene was mapped with an Eco RI polymorphism between Arabidopsis ecotypes WS and W100 with RI lines (Dupont) and is nonallelic to the DDM1 locus (4).
14. H. Rodriguez-Rodriguez, M. Ronemus, A. Calderon-Urrea, S. L. Dellaporta, unpublished data.
15. MET1 cDNA B6-2 was cloned into the Eco RI site of T-DNA vector pMON530 (Monsanto) in the antisense orientation to the CaMV 35S promoter through use of Eco RI sites present in cDNA linkers. The pMON530 T-DNA confers resistance to kanamycin (50 μg/ml) on transgenic plants.
16. In all transformations and analyses, an Arabidopsis strain Columbia line homozygous for a mutation at the gl1 locus was used as a marker (but otherwise wild type), except as noted.
17. Transformation was by the protocol of N. Bechtold, J. Ellis, and G. Pelletier [C. R. Acad. Sci. Paris 316, 1194 (1993)], with modifications by A. Bent.
18. Six single-locus lines were identified by kanamycin segregation. Southern blot analyses revealed that all six lines contained independent insertions consisting of tandem repeats of the T-DNA. The plants used in this study represent the kanamycin-resistant progeny of T1 outcrosses from single-locus lines Tr242, 243, 244, 245, 246, and 248. Before germination, all seeds were plated on MS media (Gibco) with (all transgenic lines) or without (controls) kanamycin (50 μg/ml, Sigma) and incubated for 3 days at 4°C in the dark. Growth conditions were 16 hours light, 8 hours dark at 21°C, except as noted. Seedlings were transplanted into soil 8 days after germination.
19. J. Chen and S. L. Dellaporta, in The Maize Handbook, M. Freeling and V. Walbot, Eds. (Springer-Verlag, New York, 1994), pp. 526-527; S. L. Dellaporta and M. A. Moreno, ibid., pp. 569-572.
20. J. Chen and S. L. Dellaporta, in The Maize Handbook, M. Freeling and V. Walbot, Eds. (Springer-Verlag, New York, 1994), pp. 526-527; S. L. Dellaporta and M. A. Moreno, ibid., pp. 569-572.
21. M. Nelson and M. McClelland, Nucleic Acids Res. 19, 2045 (1991).
22. 5mC(A/T)GG, showed only minor differences in wild-type DNA when probed with the centromere repeat and 5S rDNA probes (27) suggesting C(A/T)G methylation may not be prevalent.
23. A. B. Rose, A. L. Casselman, R. L. Last, Plant Physiol. 100, 582 (1992); P. S. Springer, W. R. McCombie, V. Sundaresan, R. A. Martienssen, Science 268, 877 (1995); X. W. Deng et al., Cell 71, 791 (1992); K. U. Torii et al., Plant Cell 8, 735 (1996).
24. A. B. Rose, A. L. Casselman, R. L. Last, Plant Physiol. 100, 582 (1992); P. S. Springer, W. R. McCombie, V. Sundaresan, R. A. Martienssen, Science 268, 877 (1995); X. W. Deng et al., Cell 71, 791 (1992); K. U. Torii et al., Plant Cell 8, 735 (1996).
25. A. B. Rose, A. L. Casselman, R. L. Last, Plant Physiol. 100, 582 (1992); P. S. Springer, W. R. McCombie, V. Sundaresan, R. A. Martienssen, Science 268, 877 (1995); X. W. Deng et al., Cell 71, 791 (1992); K. U. Torii et al., Plant Cell 8, 735 (1996).
26. A. B. Rose, A. L. Casselman, R. L. Last, Plant Physiol. 100, 582 (1992); P. S. Springer, W. R. McCombie, V. Sundaresan, R. A. Martienssen, Science 268, 877 (1995); X. W. Deng et al., Cell 71, 791 (1992); K. U. Torii et al., Plant Cell 8, 735 (1996).
27. 4 (pH 4.0).
28. L. S. Leutwiler, B. R. Hough-Evans, E. M. Meyerowitz, Mol. Gen. Genet. 194, 15 (1984).
29. E. Li, T. H. Bestor, R. Jaenisch, Cell 69, 915 (1992).
30. S. Shannon and D. R. Meeks-Wagner, Plant Cell 5, 639 (1993).
31. Kanamycin resistance segregated in a 1:1 ratio in all outcrosses from strong antisense lines.
32. J. E. Burn, D. J. Bagnall, J. D. Metzger, E. S. Dennis, W. J. Peacock, Proc. Natl. Acad. Sci. U.S.A. 90, 287 (1993).
33. R. Jütterman, E. Li, R. Jaenisch, ibid. 91, 11797 (1994).
34. R. S. Poethig, Science 250, 923 (1990).
35. R. A. Martienssen, A. Barkan, W. C. Taylor, M. Freeling, Genes Dev. 4, 331 (1990).
36. C. Gustafson-Brown, B. Savidge, M. F. Yanofsky, Cell 76, 131 (1994).
37. M. J. Ronemus, M. Galbiati, C. Ticknor, J. Chen, S. L. Dellaporta, data not shown.
38. Flowering time refers to the number of days elapsed from seed germination until emergence of an inflorescence bolt 0.5 to 1.0 cm in height.
39. Pool sizes for each line were as follows: wild-type, Tr242, Tr245: n = 6; Tr243 and Tr244, n = 4; Tr246, n = 11; and Tr248, n = 15.
40. T. Bestor, A. Laudano, R. Mattaliano, V. Ingram, J. Mol. Biol. 203, 971 (1988).
41. J. M. Martinez-Zapater, M. A. Estelle, C. R. Somerville, Mol. Gen. Genet. 204, 417 (1986).
42. B. R. Campell, Y. Song, T. E. Posch, C. A. Cullis, C. D. Town, Gene 112, 225 (1992).
43. D. Weigel, J. Alvarez, D. R. Smyth, M. F. Yanofsky, E. M. Meyerowitz, Cell 69, 843 (1992).
44. Wild-type Columbia with no mutations.
45. We thank V. Irish for critical reading of the manuscript and D. Li, M. Moreno, and A. Calderon-Urrea for helpful discussions. Supported by grants from the National Institutes of Health (GM38148) to S.L.D. and the National Science Council (81-0203-B001-14) and Academia Sinica, Taiwan, to J.C.