Structural and functional features of UDP-N-acetylenolpyruvylglucosamine reductase of Mycobacterium tuberculosis H37Rv

International Journal of Integrative Biology

Volumn 10, Issue 2, 2010, Pages 66-75

  Gautam, Ankur ^a   Shukla, Harish ^b   Khan, Saif ^c   Rishi, Praveen ^a   Bhakuni, Vinod ^b   Tewari, Rupinder ^a  

^a PANJAB UNIVERSITY   (India)

^b CENTRAL DRUG RESEARCH INSTITUTE   (India)

^c INTEGRAL UNIVERSITY   (India)

Author keywords

Bacterial cell wall; Denaturation; Flavin adenine dinucleotide (FAD); Peptidoglycan biosynthesis; UDP N acetylenolpyruvylglucosamine reductase (MurB)

Indexed keywords

BACTERIAL ENZYME; DIVALENT CATION; GUANIDINE; PEPTIDOGLYCAN; UDP N ACETYLENOLPYRUVYLGLUCOSAMINE REDUCTASE; UNCLASSIFIED DRUG; UREA;

ARTICLE; BACTERIAL CELL WALL; BACTERIAL GENE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME DENATURATION; ENZYME INACTIVATION; ENZYME MECHANISM; GENE EXPRESSION REGULATION; GENE OVEREXPRESSION; GENE SEQUENCE; MOLECULAR CLONING; MOLECULAR WEIGHT; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; OLIGOMERIZATION; PROTEIN EXPRESSION; STRUCTURE ACTIVITY RELATION; UDP N ACETYLENOLPYRUVYLGLUCOSAMINE REDUCTASE GENE;

EID: 84865362877     PISSN: 09738363     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (31)

1. Ahmed A, Akhtar MS, et al. (2001) Monovalent cation-induced conformational change in glucose oxidase leading to stabilization of the enzyme. Biochemistry, 40(7): 1945-1955.
2. Andrade MA, Chacon P, et al. (1993) Evaluation of secondary structure of proteins from UV circular dichroism spectra using an unsupervised learning neural network. Protein Eng., 6(4): 383-390.
3. Babajan B, Anuradha CM, et al. (2009) In silico structural characterization of Mycobacterium tuberculosis H37Rv UDP-W- acetylmuramate dehydrogenase. Int. J. Integ. Biol., 6: 12-16.
4. Barreteau H, Kovac A, et al. (2008) Cytoplasmic steps of peptidoglycan biosynthesis. FEMSMicrobiol. Rev., 32(2): 168-207.
5. Bastiaens PI, van Hoek A, et al. (1992) Molecular relaxation spectroscopy of flavin adenine dinucleotide in wild type and mutant lipoamide dehydrogenase from Azotobacter vinelandii. Biochemistry, 31(31): 7061-7068.
6. Benson TE, Filman DJ, et al. (1995) An enzyme-substrate complex involved in bacterial cell wall biosynthesis. Nat. Struct. Biol., 2(8): 644-653.
7. Benson TE, Harris MS, et al. (2001) A structural variation for MurB: X-ray crystal structure of Staphylococcus aureus UDP-W- Acetylenolpyruvylglucosamine reductase (MurB). Biochemistry, 40(8): 2340-2350.
8. Benson TE, Marquardt JL, et al. (1993) Overexpression, purification, and mechanistic study of UDP-N-acetylenolpymvylglucosamine reductase. Biochemistry, 32(8): 2024-2030.
9. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254.
10. Chen YH, Yang JT, et al. (1972) Determination of the secondary structures of proteins by circular dichroism and optical rotatory dispersion. Biochemistry, 11(22): 4120-4131.
11. Cole ST, Brosch R, et al. (1998) Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature, 393(6685): 537-544.
12. Culliton BJ (1992) Drug-resistant TB may bring epidemic. Nature, 356(6369): 473.
13. Dlialla AM, Yanchunas J Jr, et al. (1995) Steady-state kinetic mechanism of Escherichia coli UDP-N-acetylenolpyruvylglucosamine reductase. Biochemistry, 34(16): 5390-5402.
14. ElZoeiby A, Sanschagrin F, et al. (2003) Identification of novel inhibitors of Pseudomonas aeruginosa MurC enzyme derived from phage-displayed peptide libraries. J. Antimicrob. Chemother., 51(3): 531-543.
15. Green DW (2002) The bacterial cell wall as a source of antibacterial targets. Expert Opin. Ther. Targets, 6(1): 1-19.
16. Katz AH and Caufreld CE (2003) Structure-based design approaches to cell wall biosynthesis inhibitors. Curr. Pharm. Des., 9(11): 857-866.
17. Kim MK, Cho MK, et al. (2007) Crystal stmcture of UDP-N- acetylenolpyruvyl- glucosamine reductase (MurB) from Thermits caldophilus. Proteins, 66(3): 751-754.
18. Lackowicz JR (2006) Principles of Fluorescence Spectroscopy. 3ri ed. Plenum Press, New York
19. McDevitt D, Payne DJ, et al. (2002) Novel targets for the future development of antibacterial agents. J. Appi. Microbiol., 92: 28S-34S.
20. Mengin-Lecreulx D, Flouret B, et al. (1982) Cytoplasmic steps of peptidoglycan synthesis in Escherichia coli. J. Bacteriol, 151(3): 1109-1117.
21. Pucci MJ, Discotto LF, et al. (1992) Cloning and identification of the Escherichia coli murB DNA sequence, which encodes UDP-N- acetylenolpyruvoylglucosamine reductase. J. Bacteriol, 174(5): 1690-1693.
22. Reddy SG, Sacchettini JC, et al. (1995) Expression, purification, and characterization of Escherichia coli dihydrodipicolinate reductase. Biochemistry, 34(11): 3492-3501.
23. Rowland SL, Errington J, et al. (1995) The Bacillus subtilis cell- division 135-137 degrees region contains an essential orf with significant similarity to murB and a dispensable sbp gene. Gene, 164(1): 113-116.
24. Sambrook J, Fritsch EF, et al. (2000) Molecular Cloning: a laboratory manual. 3ri ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, USA
25. Sarver RW, Rogers JM, et al. (2002) Determination of ligand-MurB interactions by isothermal denaturation: application as a secondary assay to complement high throughput screening. J. Biomol. Screen., 7(1): 21-28
26. Sliafiani S, Slianna P, et al. (2005) Cloning and characterization of aspartate-beta-semialdehyde dehydrogenase from Mycobacterium tuberculosis H37Rv. J. Àppi. Microbiol., 98(4): 832-838.
27. Sylvester DR, Alvarez E, et al. (2001) Identification and characterization of UDP-N-acetylenolpymvylglucosamine reductase (MurB) from the Gram-positive pathogen Streptococcus pneumoniae. Biochem. J., 355(Pt 2): 431-435.
28. Tsuge H and Mitsuda H (1973) Studies on the molecular complex of flavins. IV. Activity and FAD-fluorescence change caused by the chemical modification of tryptophyl and tyrosyl residues in glucose oxidase. J. Biochem., 73(2): 199-206.
29. Van Soolingen D and Hermans PW (1995) Epidemiology of tuberculosis by DNA fingerprinting. Eur. Respir. J., 8(Suppl. 20): 649-656.
30. Vollmer W, Blanot D, et al (2008) Peptidoglycan structure and architecture. FEMS Microbiol. Rev. 32(2): 149-167.
31. World Health Organization (2008) Tuberculosis. Fact sheet 104. http://www.who.int/tb/publications/2008/factsheet_april08.pdf.