Identification and characterization of UDP-N-acetylenolpyruvylglucosamine reductase (MurB) from the Gram-positive pathogen Streptococcus pneumoniae

Biochemical Journal

Volumn 355, Issue 2, 2001, Pages 431-435

  Sylvester, D R ^a   Alvarez, E ^a   Patel, A ^a   Ratnam, K ^a   Kallender, H ^a   Wallis, N G ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

Author keywords

Bacterial cell wall; Peptidoglycan biosynthesis; UDP N acetylmuramate

Indexed keywords

ABSORPTION; CATALYSIS; CLONING; ENZYMES; GENES; SUBSTRATES;

ANTIBACTERIAL AGENTS;

ESCHERICHIA COLI;

FLAVOPROTEIN; OXIDOREDUCTASE; PEPTIDOGLYCAN; URIDINE DIPHOSPHATE N ACETYLGLUCOSAMINE;

ARTICLE; BACTERIAL CELL WALL; ENZYME ACTIVITY; ENZYME ANALYSIS; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; STREPTOCOCCUS PNEUMONIAE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CARBOHYDRATE DEHYDROGENASES; DNA PRIMERS; KINETICS; MOLECULAR SEQUENCE DATA; SEQUENCE HOMOLOGY, NUCLEIC ACID; SOLUBILITY; STREPTOCOCCUS PNEUMONIAE;

EID: 0035871278     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3550431     Document Type: Article

References (19)

1. Intracellular steps of bacterial cell wall peptidoglycan biosynthesis: Enzymology, antibiotics and antibiotic resistance
2. The mechanism of action of fosfomycin (phosphonomycin)
3. Cloning and identification of the Escherichia coli murB sequence which encodes UDP-Nacetylenolpyruvoylglucosamine reductase
4. The Bacillus subtilis cell-division 135-137 o, region contains an essential orf with similarity to murB and a dispensable sbp,gene
5. Overexpression, purification and mechanistic study of UDP-N-acetylenolpyruvylglucosamine reductase
6. Overproduction and one-step purification of Escherichia coli UDP-N-acetylglucosamine enolpyruvyl reductase
7. Steady state kinetic mechanism of Escherichia coli UDP-Nacetylenolpyruvylglucosamine reductase
8. An enzymesubstrate complex involved in bacterial cell wall biosynthesis
9. The structure of the substratefree form of MurB, an essential enzyme for the synthesis of bacterial cell walls
10. X-ray crystal structures of the S229A mutant and wild-type MurB in the presence of the substrate enolpyruvyl-UDP-N-acetylglucosamine at 1.8-A resolution
11. Kinetic charactedzation of wild-type and S229A mutant MurB: Evidence for the role of Ser 229 as a general acid
12. Two active forms of UDP-Nacetylglucosamine enolpyruvyltransferase in Gram-positive bacteria
13. Gappe BLAST and PSI-BLAST: A new generation of protein database search programs
14. Sequence divergence of the murB and rrfB genes from Escherichia coli and Salmonella typhimurium
15. Identification and characterization of cell wall-cell division gene clusters in pathogenic Gram-positive cocci
16. Unconventional organization of the division and cell wall gene cluster of Streptococcus pneumoniae
17. Biosynthesis of uridine-diphospho-N-acetylmuramic acid
18. + binding site on the MurB enzyme by NMR
19. + binding to predeuterated MurB: Backbone atom NMR assignments and chemical-shift changes