Persistent DNA damage measured by comet assay of sprague dawley rat lung cells after five days of inhalation exposure and 1 month post-exposure to dispersed multi-wall carbon nanotubes (MWCNTS) generated by new MWCNT aerosol generation system

Toxicological Sciences

Volumn 128, Issue 2, 2012, Pages 439-448

  Kim, Jin Sik ^a   Sung, Jae Huyck ^a   Song, Kyung Seuk ^a   Lee, Ji Hyun ^b   Kim, Sun Man ^c   Lee, Gun Ho ^c   Ahn, Kang Ho ^c   Lee, Jong Seong ^d   Shin, Jae Hoon ^d   Park, Jung Duck ^e   Yu, Il Je ^b  

^a Korea Institute of Construction Materials   (South Korea)

^b Hoseo University   (South Korea)

^c Hanyang University   (South Korea)

^d Occupational Lung Diseases Institute   (South Korea)

^e Chung Ang University   (South Korea)

Author keywords

CNTs; Inhalation; Lung DNA damage; MWCNTs; Single cell gel electrophoresis assay (Comet assay)

Indexed keywords

CARBON; MULTI WALLED NANOTUBE;

AEROSOL; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; COMET ASSAY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA DAMAGE; EXPOSURE; GENOTOXICITY; IN VIVO STUDY; INHALATION; LUNG ALVEOLUS CELL; LUNG ALVEOLUS EPITHELIUM; LUNG ALVEOLUS MACROPHAGE; LUNG LAVAGE; LUNG TOXICITY; MALE; MEMBRANE FILTER; MORTALITY; NONHUMAN; RAT; SPRAGUE DAWLEY RAT; WEIGHT CHANGE; WORKPLACE;

AEROSOLS; ANIMALS; BRONCHOALVEOLAR LAVAGE FLUID; COMET ASSAY; DNA DAMAGE; HYDROGEN PEROXIDE; INHALATION EXPOSURE; LUNG; MALE; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, ELECTRON, TRANSMISSION; NANOTUBES, CARBON; RATS; RATS, SPRAGUE-DAWLEY;

EID: 84865354439     PISSN: 10966080     EISSN: 10960929     Source Type: Journal    
DOI: 10.1093/toxsci/kfs161     Document Type: Article

References (50)

1. Ahn, K. H., Kim, S. M., and Yu, I. J. (2011). Multi-walled carbon nanotube (MWCNT) dispersion and aerosolization with hot water atomization without addition of any surfactant. Safety Health Work 2, 65-69.
2. Baughman, R. H., Zakhidov, A. A., and de Heer, W. A. (2002). Carbon nanotubes-the route towards applications. Science 297, 787-792.
3. Brown, D.M., Wilson, M.R., MacNee, W., Stone, V., and Donaldson, K. (2001). Size-dependent proinflammatory effects of ultrafine polystyrene particles: A role for surface area and oxidative stress in the enhanced activity of ultrafines. Toxicol. Appl. Pharmacol. 175, 191-199.
4. Card, J. W., Zeldin, D. C., Bonner, J. C., and Nestmann, E. R. (2008). Pulmonary applications and toxicity of engineered nanoparticles. Am. J. Physiol. Lung Cell. Mol. Physiol. 295, L400-L411.
5. Daniel, L. N., Mao, Y., Williams, A. O., and Saffiotti, U. (1995). Direct interaction between crystalline silica and DNA-a proposed model for silica carcinogenesis. Scand. J. Work Environ. Health 21(Suppl. 2), 22-26.
6. Dearfield, K. L., Thybaud, V., Cimino, M. C., Custer, L., Czich, A., Harvey, J. S., Hester, S., Kim, J. H., Kirkland, D., Levy, D. D., et al. (2011). Follow-up actions from positive results of in vitro genetic toxicity testing. Environ. Mol. Mutagen. 52, 177-204.
7. Deng, X., Jia, G., Wang, H., Sun, H., Wang, X., Yang, S., Wang, T., and Liu, Y. (2007). Translocation and fate of multi-walled carbon nanotubes in vivo. Carbon 45, 1419-1424.
8. Di Giorgio, M. L., Di Bucchianico, S., Ragnelli, A. M., Aimola, P., Santucci, S., and Poma, A. (2011). Effects of single and multi walled carbon nanotubes on macrophages: Cyto and genotoxicity and electron microscopy. Mutat. Res. 722, 20-31.
9. Fujitani, Y., Furuyama, A., and Hirano, S. (2009). Generation of airborne multi-walled carbon nanotubes for inhalation studies. Aerosol Sci. Technol. 43, 881-890.
10. Gonzales, L., Lison, D., and Kirsch-Volders, M. (2008). Genotoxicity of engineered nanomaterials: A critical review. Nanotoxicology 2, 252-273.
11. Han, J. H., Lee, E. J., Lee, J. H., So, K. P., Lee, Y. H., Bae, G. N., Lee, S-B., Ji, J. H., Cho, M. H., and Yu, I. J. (2008). Monitoring multiwalled carbon nanotube exposure in carbon nanotube research facility. Inhal. Toxicol. 20, 741-749.
12. Harper, T. E., and Vas, C. R. (2005). From polymer chemistry to nanotechnology: The return of the renaissance scientist? Abstr. Pap. Am. Chem. Soc. 229, U906.
13. Hubbard, R., Venn, A., Lewis, S., and Britton, J. (2000). Lung cancer and cryptogenic fibrosing alveolitis. A population-based cohort study. Am. J. Respir. Crit. Care Med. 161, 5-8.
14. International Conference on Harmonisation (ICH) Steering Committee. ICH Guideline S2B: Genotoxicity: A Standard Battery for Genotoxicity Testing of Pharmaceuticals. 1997 July.
15. Jaurand, M. C. (1989). Particulate-state carcinogenesis: A survey of recent studies on the mechanisms of action of fibres. IARC Sci. Publ. 90, 54-73.
16. Kim, J. S., Lee, K., Lee, Y. H., Cho, H. S., Kim, K. H., Choi, K. H., Lee, S. H., Song, K. S., Kang, C. S., and Yu, I. J. (2011). Aspect ratio has no effect on genotoxicity of multi-wall carbon nanotubes. Arch. Toxicol. 85, 775-786.
17. Kim, J. S., Song, K. S., Joo, H. J., Lee, J. H., and Yu, I. J. (2010). Determination of cytotoxicity attributed to multiwall carbon nanotubes (MWCNT) in normal human embryonic lung cell (WI-38) line. J. Toxicol. Environ. Health A. 73, 1521-1529.
18. Kisin, E. R., Murray, A. R., Keane, M. J., Shi, X. C., Schwegler-Berry, D., Gorelik, O., Arepalli, S., Castranova, V., Wallace, W. E., Kagan, V. E., et al. (2007). Single-walled carbon nanotubes: Geno-and cytotoxic effects in lung fibroblast V79 cells. J. Toxicol. Environ. Health A. 70, 2071-2079.
19. Knaapen, A. M., Borm, P. J., Albrecht, C., and Schins, R. P. (2004). Inhaled particles and lung cancer. Part A: Mechanisms. Int. J. Cancer 109, 799-809.
20. Ku, B. K., Maynard, A. D., Baron, P. A., and Deye, G. J. (2007). Observation and measurement of anomalous responses in a differential mobility analyzer caused by ultrafine fibrous carbon aerosols. J. Electrostat. 65, 542-548.
21. Kumaravel, T. S., and Jha, A. N. (2006). Reliable Comet assay measurements for detecting DNA damage induced by ionising radiation and chemicals. Mutat. Res. 605, 7-16.
22. Lam, C. W., James, J. T., McCluskey, R., and Hunter, R. L. (2004). Pulmonary toxicity of single-wall carbon nanotubes in mice 7 and 90 days after intratracheal instillation. Toxicol. Sci. 77, 126-134.
23. Lee, J. H., Lee, S. B., Bae, G. N., Jeon, K. S., Yoon, J. U., Ji, J. H., Sung, J. H., Lee. B. G., Lee, J. H., Yang, J. S., et al. (2010). Exposure assessment of carbon nanotube manufacturing workplaces. Inhal. Toxicol. 22, 369-381.
24. Li, J. G., Li, W. X., Xu, J. Y., Cai, X. Q., Liu, R. L., Li, Y. J., Zhao, Q. F., and Li, Q. N. (2007). Comparative study of pathological lesions induced by multiwalled carbon nanotubes in lungs of mice by intratracheal instillation and inhalation. Environ. Toxicol. 22, 415-421.
25. Lin, Y., Taylor, S., Li, H., Fernando, S. K. A., Qu, L., Wang, W., Gu, L., Zhou, B., and Sun, Y. P. (2004). Advances towards bioapplications of carbon nanotubes. J. Mater. Chem. 14, 527-541.
26. Lindberg, H. K., Falck, G. C. M., Suhonen, S., Vippola, M., Vanhala, E., Catalan, J., Savolainen, K., and Norppa, H. (2009). Genotoxicity of nanomaterials: DNA damage and micronuclei induced by carbon nanotubes and graphite nanofibres in human bronchial epithelial cells in vitro. Toxicol. Lett. 186, 166-173.
27. Ma-Hock, L., Treumann, S., Strauss, V., Brill, S., Luizi, F., Mertler, M., Wiench, K., Gamer, A. O., van Ravenzwaay, B., and Landsiedel, R. (2009). Inhalation toxicity of multiwall carbon nanotubes in rats exposed for 3 months. Toxicol. Sci. 112, 468-481.
28. Martin, C. R., and Kohli, P. (2003). The emerging field of nanotube biotechnology. Nat. Rev. Drug Discov. 2, 29-37.
29. Maynard, A. D., Baron, P. A., Foley, M., Shvedova, A. A., Kisin, E. R., and Castranova, V. (2004). Exposure to carbon nanotube material: Aerosol release during the handling of unrefined single walled carbon nanotube material. J. Toxicol. Environ. Health A 67, 87-107.
30. Mercer, R. R., Hubbs, A. F., Scabilloni, J. F., Wang, L., Battelli, L. A., Friend, S., Castranova, V., and Porter, D. W. (2011). Pulmonary fibrotic response to aspiration of multi-walled carbon nanotubes. Part. Fibre Tox. 8, 21.
31. Miller, F. J. (2000). Dosimetry of particles in laboratory animals and humans in relationship to issues surrounding lung overload and human health risk assessment: A critical review. Inhal. Toxicol. 12, 19-57.
32. Mitchell, L. A., Gao, J., Wal, R. V., Gigliotti, A., Burchiel, S. W., and McDonald, J. D. (2007). Pulmonary and systemic immune response to inhaled multiwalled carbon nanotubes. Toxicol. Sci. 100, 203-214.
33. Muller, J., Huaux, F., Moreau, N., Misson, P., Heilier, J. F., Delos, M., Arras, M., Fonseca, A., Nagy, J. B., and Lison, D. (2005). Respiratory toxicity of multi-wall carbon nanotubes. Toxicol. Appl. Pharmacol. 207, 221-231.
34. Muller, J., Decordier, I., Hoet, P. H., Lombaert, N., Thomassen, L., Huaux, F., Lison, D., and Kirsch-Volders, M. (2008). Clastogenic and aneugenic effects of multi-wall carbon nanotubes in epithelial cells. Carcinogenesis 29, 427-443.
35. Oberdorster, G., Oberdorster, E., and Oberdorster, J. (2005). Nanotoxicology: An emerging discipline evolving from studies of ultrafine particles. Environ. Health Perspect. 113, 823-839.
36. Pauluhn, J. (2010). Subchronic 13-week inhalation exposure of rats to multiwalled carbon nanotubes: Toxic effects are determined by density of agglomerate structures, not fibrillar structures. Toxicol. Sci. 113, 226-242.
37. Porter, D. W., Hubbs, A. F., Mercer, R. R., Wu, N., Wolfarth, M. G., Sriram, K., Leonard, S., Battelli, L., Schwegler-Berry, D., Friend, S., et al. (2010). Mouse pulmonary dose-and time course-responses induced by exposure to multi-walled carbon nanotubes. Toxicology 269, 136-147.
38. Ren, S., Zhang, L., Cheng, Z., and Guo, Y. J. (2005). Immobilized carbon nanotubes as matrix for MALDI-TOF-MS analysis: Applications to neutral small carbohydrates. Am. Soc. Mass Spectrom. 16, 333-339.
39. Ryman-Rasmussen, J. P., Cesta, M. F., Brody, A. R., Shipley-Phillips, J. K., Everitt, J. I., Tewksbury, E. W., Moss, O. R., Wong, B. A., Dodd, D. E., Andersen, M. E., et al. (2009a). Inhaled carbon nanotubes reach the subpleural tissue in mice. Nat. Nanotechnol. 4, 747-751.
40. Ryman-Rasmussen, J. P., Tewksbury, E. W., Moss, O. R., Cesta, M. F., Wong, B. A., and Bonner, J. C. (2009b). Inhaled multiwalled carbon nanotubes potentiate airway fibrosis in a murine model of allergic asthma. Am. J. Resp. Cell. Mol. Biol. 40, 349-358.
41. Sargent, L. M., Reynolds, S. H., and Castranova, V. (2010). Potential pulmonary effects of engineered carbon nanotubes: In vitro genotoxic effects. Nanotoxicology 4, 396-408.
42. Shvedova, A. A., Kisin, E. R., Mercer, R., Murray, A. R., Johnson, V. J., Potapovich, A. I., Tyurina, Y. Y., Gorelik, O., Arepalli, S., SchweglerBerry, D., et al. (2005). Unusual inflammatory and fibrogenic pulmonary responses to single-walled carbon nanotubes in mice. Am. J. Physiol. Lung Cell. Mol. Physiol. 289, L698-L708.
43. Singh, N. P., Mccoy, M. T., Tice, R. R., and Schneider, E. L. (1988). A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175, 184-191.
44. Sung, J. H., Ji, J. H., Park, J. D. Yoon, J. U., Kim, D. S., Jeon, K. S., Song, M. Y., Jeong, J., Han, B. S., Han, J. H., et al. (2009). Subchronic inhalation toxicity of silver nanoparticles. Toxicol. Sci. 108, 452-461.
45. Svensson, S., Olin, A. C., Lärstad, M., Ljungkvist, G., and Torén, K. (2004). Determination of hydrogen peroxide in exhaled breath condensate by flow injection analysis with fluorescence detection. J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 809, 199-203.
46. Takagi, A., Hirose, A., Nishimura, T., Fukumori, N., Ogata, A., Ohashi, N., Kitajima, S., and Kanno, J. (2008). Induction of mesothelioma in p53+/-mouse by intraperitoneal application of multi-wall carbon nanotubes. J. Toxicol. Sci. 33, 105-116.
47. Uno, Y. (2006). Understanding of genotoxic mechanism of action for carcinogen risk assessment to humans; a commentary to the discussion at the 4th International Workshop on Genotoxicity Testing (IWGT). Genes Environ. 28, 38-39.
48. Vaslet, C. A., Messier, N. J., and Kane, A. B. (2002). Accelerated progression of asbestos-induced mesotheliomas in heterozygous p53+/-mice. Toxicol. Sci. 68, 331-338.
49. Warheit, D. B., Laurence, B. R., Reed, K. L., Roach, D. H., Reynolds, G. A., and Webb, T. R. (2004). Comparative pulmonary toxicity assessment of single-wall carbon nanotubes in rats. Toxicol. Sci. 77, 117-125.
50. Zegura, B., and Filipic, M. (2004). Application of in vitro comet assay for genotoxicity testing. In Optimization in Drug Discovery: In Vitro Methods (Methods in Pharmacology and Toxicology). (Z. Yan and G. Caldwell, Eds.), pp. 301-313. Humana Press, Totowa, NJ.