Aspergillus PCR testing: Results from a prospective PCR study within the AmBiLoad trial

European Journal of Haematology

Volumn 85, Issue 2, 2010, Pages 164-169

  Hummel, Margit ^a   Spiess, Birgit ^a   Cornely, Oliver A ^b   Dittmer, Martin ^c   Mörz, Handan ^a   Buchheidt, Dieter ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b UNIVERSITY OF COLOGNE   (Germany)

^c Gilead Sciences GmbH   (Germany)

Author keywords

Aspergillus PCR; hematologic malignancies; invasive aspergillosis; invasive fungal infection

Indexed keywords

AMPHOTERICIN B LIPID COMPLEX; FUNGAL DNA; FUNGUS ANTIGEN; GALACTOMANNAN;

ADOLESCENT; ADULT; AGED; ARTICLE; ASPERGILLUS; BLOOD SAMPLING; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DISEASE SEVERITY; DNA DETERMINATION; FEMALE; HUMAN; IMMUNOCOMPROMISED PATIENT; MAJOR CLINICAL STUDY; MALE; MICROORGANISM DETECTION; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROGNOSIS; PROSPECTIVE STUDY; RANDOMIZED CONTROLLED TRIAL; SEROLOGY; SYSTEMIC MYCOSIS; TREATMENT DURATION; TREATMENT OUTCOME;

ADOLESCENT; ADULT; AGED; AMPHOTERICIN B; ANTIFUNGAL AGENTS; ASPERGILLOSIS; ASPERGILLUS; DNA, FUNGAL; HUMANS; IMMUNOCOMPROMISED HOST; MIDDLE AGED; POLYMERASE CHAIN REACTION; PROGNOSIS; PROSPECTIVE STUDIES; SENSITIVITY AND SPECIFICITY; YOUNG ADULT;

EID: 77954739740     PISSN: 09024441     EISSN: 16000609     Source Type: Journal    
DOI: 10.1111/j.1600-0609.2010.01452.x     Document Type: Article

References (28)

1. Cornely OA, Ullmann AJ, Karthaus M. Evidence-based assessment of primary antifungal prophylaxis in patients with hematologic malignancies. Blood 2003 101 : 3365 3372.
2. Boudewijns M, Verweij PE, Melchers WJ. Molecular diagnosis of invasive aspergillosis: the long and winding road. Future Microbiol 2006 1 : 283 293.
3. Morace G, Sanguinetti M, Posteraro B, Lo CG, Fadda G. Identification of various medically important Candida species in clinical specimens by PCR-restriction enzyme analysis. J Clin Microbiol 1997 35 : 667 672.
4. von Eiff M, Roos N, Schulten R, Hesse M, Zuhlsdorf M, van de LJ. Pulmonary aspergillosis: early diagnosis improves survival. Respiration 1995 62 : 341 347.
5. Denning DW. Invasive aspergillosis. Clin Infect Dis 1998 26 : 781 803.
6. Buchheidt D, Hummel M. Aspergillus polymerase chain reaction (PCR) diagnosis. Med Mycol 2005 43 : 139 145.
7. White PL, Linton CJ, Perry MD, Johnson EM, Barnes RA. The evolution and evaluation of a whole blood polymerase chain reaction assay for the detection of invasive aspergillosis in hematology patients in a routine clinical setting. Clin Infect Dis 2006 42 : 479 486.
8. Florent M, Katsahian S, Vekhoff A, Levy V, Rio B, Marie JP, Bouvet A, Comet M. Prospective evaluation of a polymerase chain reaction-ELISA targeted to Aspergillus fumigatus and Aspergillus flavus for the early diagnosis of invasive aspergillosis in patients with hematological malignancies. J Infect Dis 2006 193 : 741 747.
9. Halliday C, Hoile R, Sorrell T, James G, Yadav S, Shaw P, Bleakley M, Bradstock K, Chen S. Role of prospective screening of blood for invasive aspergillosis by polymerase chain reaction in febrile neutropenic recipients of haematopoietic stem cell transplants and patients with acute leukaemia. Br J Haematol 2006 132 : 478 486.
10. Jordanides NE, Allan EK, McLintock LA, Copland M, Devaney M, Stewart K, Parker AN, Johnson PR, Holyoake TL, Jones BL. A prospective study of real-time panfungal PCR for the early diagnosis of invasive fungal infection in haemato-oncology patients. Bone Marrow Transplant 2005 35 : 389 395.
11. Donnelly JP. Polymerase chain reaction for diagnosing invasive aspergillosis: getting closer but still a ways to go. Clin Infect Dis 2006 42 : 487 489.
12. Cornely OA, Maertens J, Bresnik M, et al. Liposomal amphotericin B as initial therapy for invasive mold infection: a randomized trial comparing a high-loading dose regimen with standard dosing (AmBiLoad trial). Clin Infect Dis 2007 44 : 1289 1297.
13. Skladny H, Buchheidt D, Baust C, Krieg-Schneider F, Seifarth W, Leib-Mosch C, Hehlmann R. Specific detection of Aspergillus species in blood and bronchoalveolar lavage samples of immunocompromised patients by two-step PCR. J Clin Microbiol 1999 37 : 3865 3871.
14. Ascioglu S, Rex JH, de Pauw B, et al. Defining opportunistic invasive fungal infections in immunocompromised patients with cancer and hematopoietic stem cell transplants: an international consensus. Clin Infect Dis 2002 34 : 7 14.
15. Herbrecht R, Denning DW, Patterson TF, et al. Voriconazole versus amphotericin B for primary therapy of invasive aspergillosis. N Engl J Med 2002 347 : 408 415. (Pubitemid 34851833)
16. Spiess B, Buchheidt D, Baust C, Skladny H, Seifarth W, Zeilfelder U, Leib-Mosch C, Morz H, Hehlmann R. Development of a LightCycler PCR assay for detection and quantification of Aspergillus fumigatus DNA in clinical samples from neutropenic patients. J Clin Microbiol 2003 41 : 1811 1818.
17. Hope WW, Walsh TJ, Denning DW. Laboratory diagnosis of invasive aspergillosis. Lancet Infect Dis 2005 5 : 609 622.
18. Lass-Florl C, Gunsilius E, Gastl G, Bonatti H, Freund MC, Gschwendtner A, Kropshofer G, Dierich MP, Petzer A. Diagnosing invasive aspergillosis during antifungal therapy by PCR analysis of blood samples. J Clin Microbiol 2004 42 : 4154 4157.
19. Einsele H, Hebart H, Roller G, et al. Detection and identification of fungal pathogens in blood by using molecular probes. J Clin Microbiol 1997 35 : 1353 1360.
20. Kami M, Fukui T, Ogawa S, et al. Use of real-time PCR on blood samples for diagnosis of invasive aspergillosis. Clin Infect Dis 2001 33 : 1504 1512.
21. O'Sullivan CE, Kasai M, Francesconi A, Petraitis V, Petraitiene R, Kelaher AM, Sarafandi AA, Walsh TJ. Development and validation of a quantitative real-time PCR assay using fluorescence resonance energy transfer technology for detection of Aspergillus fumigatus in experimental invasive pulmonary aspergillosis. J Clin Microbiol 2003 41 : 5676 5682.
22. Bowman JC, Abruzzo GK, Anderson JW, Flattery AM, Gill CJ, Pikounis VB, Schmatz DM, Liberator PA, Douglas CM. Quantitative PCR assay to measure Aspergillus fumigatus burden in a murine model of disseminated aspergillosis: demonstration of efficacy of caspofungin acetate. Antimicrob Agents Chemother 2001 45 : 3474 3481.
23. Marr KA, Laverdiere M, Gugel A, Leisenring W. Antifungal therapy decreases sensitivity of the Aspergillus galactomannan enzyme immunoassay. Clin Infect Dis 2005 40 : 1762 1769.
24. Petraitiene R, Petraitis V, Groll AH, Sein T, Schaufele RL, Francesconi A, Bacher J, Avila NA, Walsh TJ. Antifungal efficacy of caspofungin (MK-0991) in experimental pulmonary aspergillosis in persistently neutropenic rabbits: pharmacokinetics, drug disposition, and relationship to galactomannan antigenemia. Antimicrob Agents Chemother 2002 46 : 12 23.
25. Bhatti Z, Shaukat A, Almyroudis NG, Segal BH. Review of epidemiology, diagnosis, and treatment of nvasive mould infections in allogeneic hematopoietic stem cell transplant recipients. Mycopathologia 2006 162 : 1 15.
26. Upton A, Marr KA. Emergence of opportunistic mould infections in the hematopoietic stem cell transplant patient. Curr Infect Dis Rep 2006 8 : 434 441.
27. Chamilos G, Luna M, Lewis RE, Bodey GP, Chemaly R, Tarrand JJ, Safdar A, Raad II, Kontoyiannis DP. Invasive fungal infections in patients with hematologic malignancies in a tertiary care cancer center: an autopsy study over a 15-year period (1989-2003). Haematologica 2006 91 : 986 989.
28. Marr KA, Carter RA, Crippa F, Wald A, Corey L. Epidemiology and outcome of mould infections in hematopoietic stem cell transplant recipients. Clin Infect Dis 2002 34 : 909 917. (Pubitemid 34229758)