Diagnosing invasive aspergillosis during antifungal therapy by PCR analysis of blood samples

Journal of Clinical Microbiology

Volumn 42, Issue 9, 2004, Pages 4154-4157

  Lass Flörl, Cornelia ^a,c   Gunsilius, Eberhard ^b   Gastl, Günther ^b   Bonatti, Hugo ^b   Freund, Martin C ^b   Gschwendtner, Andreas ^a   Kropshofer, Gabriele ^b   Dierich, Manfred P ^a   Petzer, Andreas ^b  

^a UNIVERSITY OF INNSBRUCK   (Austria)

^b UNIVERSITY HOSPITAL INNSBRUCK   (Austria)

^c INNSBRUCK MEDICAL UNIVERSITY   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

AMPHOTERICIN B; AMPHOTERICIN B LIPID COMPLEX; ANTIFUNGAL AGENT; CASPOFUNGIN; FUNGAL DNA; ITRACONAZOLE; VORICONAZOLE;

ADULT; AGED; ARTICLE; ASPERGILLOSIS; ASPERGILLUS; ASPIRATION BIOPSY; BLOOD; CLINICAL ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; FUNGUS CULTURE; HUMAN; HUMAN TISSUE; LUNG; LUNG LAVAGE; MALE; MICROSCOPY; PNEUMONIA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; THORAX RADIOGRAPHY;

ADULT; AGED; AMPHOTERICIN B; ANTIFUNGAL AGENTS; ASPERGILLOSIS; ASPERGILLUS; BASE SEQUENCE; BRONCHOALVEOLAR LAVAGE FLUID; DNA PRIMERS; DNA, FUNGAL; HUMANS; LEUKEMIA; MIDDLE AGED; NEOPLASMS; POLYMERASE CHAIN REACTION; PYRIMIDINES; TRANSPLANTATION; TRIAZOLES;

ASPERGILLUS; FUNGI;

EID: 4644228689     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.42.9.4154-4157.2004     Document Type: Article

References (26)

1. Aisner, J., P. H. Wiernik, and S. C. Schimpff. 1977. Treatment of invasive aspergillosis: relation of early diagnosis and treatment to response. Ann. Intern. Med. 86:539-543.
2. Anaissie, E. 1992. Opportunistic mycoses in the immunocompromised host: experience at a cancer center and review. Clin. Infect. Dis. 14(Suppl. 1):43-53.
3. Ascioglu, S., J. H. Rex, B. de Pauw, J. E. Bennett, J. Bille, F. Crokaert, D. W. Denning, P. Donnelly, J. E. Edwards, Z. Erjavec, D. Fiere, O. Lortholary, J. Maertens, J. F. Meis, T. F. Patterson, J. Ritter, D. Selleslag, M. Shah, D. A. Stevens, and T. J. Walsh. 2002. Defining opportunistic invasive fungal infections in immunocompromised patients with cancer and hematopoetic stem cell transplants: an international consensus. Clin. Infect. Dis. 34:7-14.
4. Bialek, R., D. Moshous, J. L. Casanova, S. Blanche, and C. Hennequin. 2002. Aspergillus antigen and PCR assays in bone marrow transplanted children. Eur. J. Med. Res. 7:177-180.
5. Bretagne, S., J. M. Costa, A. Marmor-Khuong, F. Poron, C. Cordonnier, M. Vidaud, and J. Fleur-Feith. 1995. Detection of Aspergillus species DNA in bronchoalveolar lavage samples by competitive PCR. J. Clin. Microbiol. 33:1164-1168.
6. Bretagne, S., J. Costa, E. Bart-Delabesse, N. Dhedin, C. Rieux, and C. Cordonnier. 1998. Comparison of serum galactomannan antigen detection and competitive polymerase chain reaction for diagnosing invasive aspergillosis. Clin. Infect. Dis. 26:1407-1412.
7. Buchheidt, D., C. Baust, H. Skladny, M. Baldus, S. Bräuninger, and R. Hehlmann. 2002. Clinical evaluation of a polymerase chain reaction assay to detect Aspergillus species in bronchoalveolar lavage samples of neutropenic patients. Br. J. Haematol. 116:803-811.
8. Buchheidt, D., M. Hummel, D. Schleiermacher, B. Spiess, R. Schwerdtfeger, O. Cornely, S. Wilhelm, S. Reuter, W. Kern, T. Südhoff, H. Mörz, and R. Hehlmann. 2004. Prospective clinical evaluation of LightCycler™- mediated polymerase chain reaction assay, a nested-PCR assay and galactomannan enzyme-linked immunosorbent assay for detection of invasive aspergillosis in neutropenic cancer patients and haematological stem cell transplant recipients. Br. J. Haematol. 125:196-202.
9. Denning, D. W., S. E. Follansbee, M. Scolaro, S. Norris, H. Edelstein, and D. Stevens. 1991. Pulmonary aspergillosis in the acquired immunodeficiency syndrome. N. Engl. J. Med. 324:654-662.
10. Duthie, R., and D. W. Denning. 1995. Aspergillus fungemia: report of two cases and review. Clin. Infect. Dis. 20:598-605.
11. Einsele, H., H. Hebart, G. Roller, J. Löffler, I. Rothenhöfer, C. Müller, R. Bowden, J. A. Burik, D. Engelhard, L. Kanz, and U. Schumacher. 1997. Detection and identification of fungal pathogens in blood by using molecular probes. J. Clin. Microbiol. 35:1353-1360.
12. Einsele, H., K. Quabeck, K. D. Müller, H. Hebart, I. Rothenhöfer, J. Löffler, and U. W. Schaefer. 1998. Prediction of invasive pulmonary aspergillosis from colonization of lower respiratory-tract before marrow transplantation. Lancet 358:1443.
13. Ferns, R. B., H. Fletcher, S. Bradley, S. Mackinnon, C. Hunt, and R. S. Tedder. 2002. The prospective evaluation of a nested polymerase chain reaction assay for the early detection of Aspergillus infection in patients with leukaemia or undergoing allograft treatment. Br. J. Haematol. 119:720-725.
14. Herbrecht, R., V. Letscher-Bru, C. Oprea, B. Lioure, J. Waller, F. Campos, O. Villard, K. Liu, S. Natajan-Ame, P. Lutz, J. P. Bergerat, and E. Candolfi. 2002. Aspergillus galactomannan detection in the diagnosis of invasive aspergillosis in cancer patients. J. Clin. Oncol. 20:1898-1906.
15. Lass-Flörl, C., J. Aigner, G. Eberhard, A. Petzer, D. Nachbaur, G. Gastl, H. Einsele, J. Löffler, M. P. Dierich, and R. Würzner. 2001. Screening for Aspergillus spp. using polymerase chain reaction of whole blood samples from patients with haematological malignancies. Br. J. Haematol. 113:180-184.
16. Lass-Flörl, C., C. Speth, A. Mayr, R. Würzner, M. P. Dierich, H. Ulmer, and H. Dietrich. 2003. Diagnosing and monitoring of invasive aspergillosis during antifungal therapy by polymerase chain reaction: an experimental study in mice. Diagn. Microbiol. Infect. Dis. 42:569-572.
17. Loeffler, J., H. Hebart, U. Brauchte, U. Schumacher, and H. Einsele. 2000. Comparison between plasma and whole blood specimens for detection of Aspergillus DNA by PCR. J. Clin. Microbiol. 38:3830-3833.
18. Loeffler, J., K. Kloepfer, H. Hebart, L. Najvar, J. R. Graybill, W. R. Kirkpatrick, T. F. Patterson, K. Dietz, R. Bialek, and H. Einsele. 2002. Polymerase chain reaction detection of Aspergillus DNA in experimental models of invasive aspergillosis. J. Infect. Dis. 185:1203-1206.
19. Löffler, J., H. Hebart, S. Sepe, U. Schumacher, T. Klingebiel, and H. Einsele. 1998. Detection of PCR-amplified fungal DNA by using a PCR-ELISA system. Med. Mycol. 36:275-279.
20. Maertens, J., J. Van Eldere, J. Verhaegen, E. Verbeken, J. Verschakelen, and M. Boogaerts. 2002. Use of circulating galactomannan screening for early diagnosis of invasive aspergillosis in allogeneic stem cell transplant recipients. J. Infect. Dis. 186:1297-1306.
21. Peterson, P. K., P. McGlave, N. K. Ramsay, F. Rhame, E. Cohen, G. S. Perry, A. J. Goldman, and J. Kersey. 1983. A prospective study of infectious diseases following bone marrow transplantation: emergence of Aspergillus and Cytomegalovirus as the major causes of mortality. Infect. Control 4:81-89.
22. Ruhnke, M., A. Bohme, D. Buchheidt, K. Donhuijsen, H. Einsele, R. Enzensberger, A. Glasmacher, H. Gumbel, C. P. Heussel, M. Karthaus, E. Lambrecht, T. Sudhoff, and H. Szelenyl. 2003. Diagnosis of invasive fungal infections in hematology and oncology-guidelines of the Infectious Diseases Working Party (AGHO) of the German Society of Hematology and Oncology (DGHO). Ann. Hematol. 82(Suppl. 2):S141-S148.
23. Rumore, P., B. Muralidhar, M. Lin, C. Lai, and C. Steinman. 1992. Haemodialysis as a model for studying endogenous plasma DNA: oligonucleosome-like structure and clearance. Clin. Exp. Immunol. 90:56-62.
24. Skladny, H., D. Buchheidt, C. Baust, F. Krieg-Schneider, W. Seifarth, C. Leih-Mösch, and R. Hehlmann. 1999. Specific detection of Aspergillus species in blood and bronchoalveolar lavage samples of immunocompromised patients by two-step PCR. J. Clin. Microbiol. 37:3865-3871.
25. Williams, E., J. P. Leeming, H. M. Palmer, C. G. Steward, D. Warnock, D. I. Marks, and M. R. Millar. 2000. Diagnosis of invasive aspergillosis in bone marrow transplant recipients by polymerase chain reaction. Br. J. Haematol. 108:132-139.
26. Yamakami, Y., A. Hashimoto, E. Yamagata, P. Kamheri, R. Karashima, H. Nagai, and M. Nasu. 1998. Evaluation of PCR for detection of DNA specific for Aspergillus species in sera of patients with various forms of pulmonary aspergillosis. J. Clin. Microbiol. 36:3619-3623.