Entamoeba histolytica: A snapshot of current research and methods for genetic analysis

Current Opinion in Microbiology

Volumn 15, Issue 4, 2012, Pages 469-475

  Morf, Laura ^a   Singh, Upinder ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTOZOAL RNA;

AMEBIASIS; ENTAMOEBA HISTOLYTICA; EVOLUTION; GENETIC ANALYSIS; HOST PARASITE INTERACTION; HUMAN; MOLECULAR BIOLOGY; NONHUMAN; PROTOZOAL GENETICS; REVIEW;

ENTAMOEBA HISTOLYTICA; ENTAMOEBIASIS; HOST-PARASITE INTERACTIONS; HUMANS; PROTOZOAN PROTEINS; RNA INTERFERENCE; VIRULENCE FACTORS;

ENTAMOEBA; ENTAMOEBA HISTOLYTICA; EUKARYOTA; PROTOZOA;

EID: 84865291103     PISSN: 13695274     EISSN: 18790364     Source Type: Journal    
DOI: 10.1016/j.mib.2012.04.011     Document Type: Review

References (54)

1. WHO WHO/PAHO/UNESCO report. A consultation with experts on amoebiasis. Mexico City, Mexico, 28-29 January 1997. Epidemiol Bull 1997, 18:13-14.
2. Haque R., Huston C.D., Hughes M., Houpt E., Petri W.A. Amebiasis. N Engl J Med 2003, 348:1565-1573.
3. Samuelson J., Robbins P. A simple fibril and lectin model for cyst walls of Entamoeba and perhaps Giardia. Trends Parasitol 2011, 27:17-22.
4. Haque R., Kabir M., Noor Z., Rahman S.M., Mondal D., Alam F., Rahman I., Al Mahmood A., Ahmed N., Petri W.A. Diagnosis of amebic liver abscess and amebic colitis by detection of Entamoeba histolytica DNA in blood, urine, and saliva by a real-time PCR assay. J Clin Microbiol 2010, 48:2798-2801.
5. Haque R., Mondal D., Duggal P., Kabir M., Roy S., Farr B.M., Sack R.B., Petri W.A. Entamoeba histolytica infection in children and protection from subsequent amebiasis. Infect Immun 2006, 74:904-909.
6. Ralston K.S., Petri W.A. Tissue destruction and invasion by Entamoeba histolytica. Trends Parasitol 2011, 27:254-263.
7. Duggal P., Guo X., Haque R., Peterson K.M., Ricklefs S., Mondal D., Alam F., Noor Z., Verkerke H.P., Marie C., et al. A mutation in the leptin receptor is associated with Entamoeba histolytica infection in children. J Clin Invest 2011, 121:1191-1198.
8. Guo X., Roberts M.R., Becker S.M., Podd B., Zhang Y., Chua S.C., Myers M.G., Duggal P., Houpt E.R., Petri W.A. Leptin signaling in intestinal epithelium mediates resistance to enteric infection by Entamoeba histolytica. Mucosal Immunol 2011, 4:294-303.
9. Furukawa A., Nakada-Tsukui K., Nozaki T. Novel transmembrane receptor involved in phagosome transport of lysozymes and beta-hexosaminidase in the enteric protozoan Entamoeba histolytica. PLoS Pathog 2012, 8:e1002539.
10. Teixeira J.E., Sateriale A., Bessoff K.E., Huston C.D. Control of Entamoeba histolytica adherence involves EhMSP-1, a M8 family surface metalloprotease with homology to leishmanolysin. Infection Immun 2012, 80:2165-2176.
11. Biller L., Davis P.H., Tillack M., Matthiesen J., Lotter H., Stanley S.L., Tannich E., Bruchhaus I. Differences in the transcriptome signatures of two genetically related Entamoeba histolytica cell lines derived from the same isolate with different pathogenic properties. BMC Genomics 2010, 11:63.
12. Shrimal S., Bhattacharya S., Bhattacharya A Serum-dependent selective expression of EhTMKB1-9, a member of Entamoeba histolytica B1 family of transmembrane kinases. PLoS Pathog 2010, 6:e1000929.
13. Cavalier-Smith T. A revised six-kingdom system of life. Biol Rev Camb Philos Soc 1998, 73:203-266.
14. Tovar J., Fischer A., Clark C.G. The mitosome, a novel organelle related to mitochondria in the amitochondrial parasite Entamoeba histolytica. Mol Microbiol 1999, 32:1013-1021.
15. Dolezal P., Dagley M.J., Kono M., Wolynec P., Likić V.A., Foo J.H., Sedinová M., Tachezy J., Bachmann A., Bruchhaus I., Lithgow T. The essentials of protein import in the degenerate mitochondrion of Entamoeba histolytica. PLoS Pathog 2010, 6:e1000812.
16. Tovar J., León-Avila G., Sánchez L.B., Sutak R., Tachezy J., van der Giezen M., Hernández M., Müller M., Lucocq J.M. Mitochondrial remnant organelles of Giardia function in iron-sulphur protein maturation. Nature 2003, 426:172-176.
17. Goldberg A.V., Molik S., Tsaousis A.D., Neumann K., Kuhnke G., Delbac F., Vivares C.P., Hirt R.P., Lill R., Embley T.M. Localization and functionality of microsporidian iron-sulphur cluster assembly proteins. Nature 2008, 452:624-628.
18. Sutak R., Dolezal P., Fiumera H.L., Hrdy I., Dancis A., Delgadillo-Correa M., Johnson P.J., Müller M., Tachezy J. Mitochondrial-type assembly of FeS centers in the hydrogenosomes of the amitochondriate eukaryote Trichomonas vaginalis. Proc Natl Acad Sci U S A 2004, 101:10368-10373.
19. Maralikova B., Ali V., Nakada-Tsukui K., Nozaki T., van der Giezen M., Henze K., Tovar J. Bacterial-type oxygen detoxification and iron-sulfur cluster assembly in amoebal relict mitochondria. Cell Microbiol 2010, 12:331-342.
20. Ali V., Shigeta Y., Tokumoto U., Takahashi Y., Nozaki T. An intestinal parasitic protist, Entamoeba histolytica, possesses a non-redundant nitrogen fixation-like system for iron-sulfur cluster assembly under anaerobic conditions. J Biol Chem 2004, 279:16863-16874.
21. van der Giezen M., Cox S., Tovar J The iron-sulfur cluster assembly genes iscS and iscU of Entamoeba histolytica were acquired by horizontal gene transfer. BMC Evol Biol 2004, 4:7.
22. Zhang H., Ehrenkaufer G.M., Pompey J.M., Hackney J.A., Singh U. Small RNAs with 5'-polyphosphate termini associate with a Piwi-related protein and regulate gene expression in the single-celled eukaryote Entamoeba histolytica. PLoS Pathog 2008, 4:e1000219.
23. Zhang H., Alramini H., Tran V., Singh U. Nucleus-localized antisense small RNAs with 5'-polyphosphate termini regulate long term transcriptional gene silencing in Entamoeba histolytica G3 strain. J Biol Chem 2011, 286:44467-44479.
24. Zhang H., Pompey J.M., Singh U. RNA interference in Entamoeba histolytica: implications for parasite biology and gene silencing. Future Microbiol 2011, 6:103-117.
25. Sijen T., Steiner F.A., Thijssen K.L., Plasterk R.H. Secondary siRNAs result from unprimed RNA synthesis and form a distinct class. Science 2007, 315:244-247.
26. Pak J., Fire A. Distinct populations of primary and secondary effectors during RNAi in C. elegans. Science 2007, 315:241-244.
27. Aoki K., Moriguchi H., Yoshioka T., Okawa K., Tabara H. In vitro analyses of the production and activity of secondary small interfering RNAs in C. elegans. EMBO J 2007, 26:5007-5019.
28. Yakoob J., Abbas Z., Beg M.A., Naz S., Khan R., Jafri W. Entamoeba species associated with chronic diarrhoea in Pakistan. Epidemiol Infect 2012, 140:323-328.
29. Ehrenkaufer G.M., Haque R., Hackney J.A., Eichinger D.J., Singh U. Identification of developmentally regulated genes in Entamoeba histolytica: insights into mechanisms of stage conversion in a protozoan parasite. Cell Microbiol 2007, 9:1426-1444.
30. Hackney J.A., Ehrenkaufer G.M., Singh U. Identification of putative transcriptional regulatory networks in Entamoeba histolytica using Bayesian inference. Nucleic Acids Res 2007, 35:2141-2152.
31. Vicente J.B., Ehrenkaufer G.M., Saraiva L.M., Teixeira M., Singh U. Entamoeba histolytica modulates a complex repertoire of novel genes in response to oxidative and nitrosative stresses: implications for amebic pathogenesis. Cell Microbiol 2009, 11:51-69.
32. Lorenzi H.A., Puiu D., Miller J.R., Brinkac L.M., Amedeo P., Hall N., Caler E.V. New assembly, reannotation and analysis of the Entamoeba histolytica genome reveal new genomic features and protein content information. PLoS Negl Trop Dis 2010, 4:e716.
33. MacFarlane R.C., Singh U. Identification of differentially expressed genes in virulent and nonvirulent Entamoeba species: potential implications for amebic pathogenesis. Infection Immun 2006, 74:340-351.
34. Davis P.H., Schulze J., Stanley S.L. Transcriptomic comparison of two Entamoeba histolytica strains with defined virulence phenotypes identifies new virulence factor candidates and key differences in the expression patterns of cysteine proteases, lectin light chains, and calmodulin. Mol Biochem Parasitol 2007, 151:118-128.
35. Singh N., Ojha S., Bhattacharya A., Bhattacharya S. Establishment of a transient transfection system and expression of firefly luciferase in Entamoeba invadens. Mol Biochem Parasitol 2012, 183:90-93.
36. Singh N., Ojha S., Bhattacharya A., Bhattacharya S. Stable transfection and continuous expression of heterologous genes in Entamoeba invadens. Mol Biochem Parasitol 2012, 184:9-12.
37. Ehrenkaufer G.M., Singh U. Transient and stable transfection in the protozoan parasite Entamoeba invadens. Mol Biochem Parasitol 2012, 184:59-62. 10.1016/j.molbiopara.2012.04.007.
38. Girard-Misguich F., Cognie J., Delgado-Ortega M., Berthon P., Rossignol C., Larcher T., Melo S., Bruel T., Guibon R., Chérel Y. Towards the establishment of a porcine model to study human amebiasis. PloS One 2011, 6:e28795.
39. Abd Alla M.D., Wolf R., White G.L., Kosanke S.D., Cary D., Verweij J.J., Zhang M.J., Ravdin J.I. Efficacy of a Gal-lectin subunit vaccine against experimental Entamoeba histolytica infection and colitis in baboons (Papio sp.). Vaccine 2012, 30:3068-3075.
40. Baxt L.A., Rastew E., Bracha R., Mirelman D., Singh U. Downregulation of an Entamoeba histolytica rhomboid protease reveals roles in regulating parasite adhesion and phagocytosis. Eukaryot Cell 2010, 9:1283-1293.
41. Bansal D., Ave P., Kerneis S., Frileux P., Boché O., Baglin A.C., Dubost G., Leguern A.S., Prevost M.C., Bracha R., et al. An ex vivo human intestinal model to study Entamoeba histolytica pathogenesis. PLoS Negl Trop Dis 2009, 3:e551.
42. Thibeaux R., Dufour A., Roux P., Bernier M., Baglin A.C., Frileux P., Olivo-Marin J.C., Guillén N., Labruyère E. Newly visualized fibrillar collagen scaffolds dictate Entamoeba histolytica invasion route in the human colon. Cell Microbiol 2012, 14:609-621.
43. Kaur G., Lohia A. Inhibition of gene expression with double strand RNA interference in Entamoeba histolytica. Biochem Biophys Res Commun 2004, 320:1118-1122.
44. Dastidar P.G., Majumder S., Lohia A. Eh Klp5 is a divergent member of the kinesin 5 family that regulates genome content and microtubular assembly in Entamoeba histolytica. Cell Microbiol 2007, 9:316-328.
45. Dastidar P.G., Lohia A. Bipolar spindle frequency and genome content are inversely regulated by the activity of two N-type kinesins in Entamoeba histolytica. Cell Microbiol 2008, 10:1559-1571.
46. MacFarlane R.C., Singh U. Loss of dsRNA-based gene silencing in Entamoeba histolytica: implications for approaches to genetic analysis. Exp Parasitol 2008, 119:296-300.
47. Linford A.S., Moreno H., Good K.R., Zhang H., Singh U., Petri W.A. Short hairpin RNA-mediated knockdown of protein expression in Entamoeba histolytica. BMC Microbiol 2009, 9:38.
48. Solis C.F., Santi-Rocca J., Perdomo D., Weber C., Guillen N. Use of bacterially expressed dsRNA to downregulate Entamoeba histolytica gene expression. PloS One 2009, 4:e8424.
49. Solis C.F., Guillen N. Silencing genes by RNA interference in the protozoan parasite Entamoeba histolytica. Methods Mol Biol 2008, 442:113-128.
50. Anbar M., Bracha R., Nuchamowitz Y., Li Y., Florentin A., Mirelman D. Involvement of a short interspersed element in epigenetic transcriptional silencing of the amoebapore gene in Entamoeba histolytica. Eukaryot Cell 2005, 4:1775-1784.
51. Bracha R., Nuchamowitz Y., Wender N., Mirelman D. Transcriptional gene silencing reveals two distinct groups of Entamoeba histolytica Gal/GalNAc-lectin light subunits. Eukaryot Cell 2007, 6:1758-1765.
52. Bracha R., Nuchamowitz Y., Anbar M., Mirelman D. Transcriptional silencing of multiple genes in trophozoites of Entamoeba histolytica. PLoS Pathog 2006, 2:e48.
53. Bracha R., Nuchamowitz Y., Leippe M., Mirelman D. Antisense inhibition of amoebapore expression in Entamoeba histolytica causes a decrease in amoebic virulence. Mol Microbiol 1999, 34:463-472.
54. Huguenin M., Bracha R., Chookajorn T., Mirelman D. Epigenetic transcriptional gene silencing in Entamoeba histolytica: insight into histone and chromatin modifications. Parasitology 2010, 137:619-627.