Diagnosis of amebic liver abscess and amebic colitis by detection of Entamoeba histolytica DNA in blood, urine, and saliva by a real-time PCR assay

Journal of Clinical Microbiology

Volumn 48, Issue 8, 2010, Pages 2798-2801

  Haque, Rashidul ^a   Kabir, Mamun ^a   Noor, Zannatun ^a   Rahman, S M Mazidur ^a   Mondal, Dinesh ^a   Alam, Faisal ^b   Rahman, Intekhab ^b   Al Mahmood, Abdullh ^c   Ahmed, Nooruddin ^c   Petri Jr , William A ^d  

^a INTERNATIONAL CENTRE FOR DIARRHOEAL DISEASE RESEARCH   (Bangladesh)

^b RAJSHAHI MEDICAL COLLEGE   (Bangladesh)

^c BANGABANDHU SHEIKH MUJIB MEDICAL UNIVERSITY   (Bangladesh)

^d UNIVERSITY OF VIRGINIA HEALTH SYSTEM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTOZOAL DNA;

AMEBIASIS; ARTICLE; BLOOD ANALYSIS; CONTROLLED STUDY; ENTAMOEBA HISTOLYTICA; FEMALE; HUMAN; LIVER ABSCESS; MAJOR CLINICAL STUDY; MALE; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SALIVA ANALYSIS; SENSITIVITY ANALYSIS; URINALYSIS; ADULT; BANGLADESH; BLOOD; DYSENTERY, AMEBIC; EVALUATION STUDY; GENETICS; ISOLATION AND PURIFICATION; LIVER ABSCESS, AMEBIC; MIDDLE AGED; PARASITOLOGY; POLYMERASE CHAIN REACTION; PROCEDURES; SALIVA; SENSITIVITY AND SPECIFICITY; URINE;

ADULT; BANGLADESH; BLOOD; DNA, PROTOZOAN; DYSENTERY, AMEBIC; ENTAMOEBA HISTOLYTICA; FEMALE; HUMANS; LIVER ABSCESS, AMEBIC; MALE; MIDDLE AGED; PARASITOLOGY; POLYMERASE CHAIN REACTION; SALIVA; SENSITIVITY AND SPECIFICITY; URINE;

ENTAMOEBA HISTOLYTICA;

EID: 77955437086     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00152-10     Document Type: Article

References (26)

1. Abd-Alla, M. D., T. F. H. G. Jackson, S. Reddy, and J. I. Ravdin. 2000. Diagnosis of invasive amebiasis by enzyme-linked immunosorbent assay of saliva to detect amebic lectin antigen and anti-lectin immunoglobulin G antibodies. J. Clin. Microbiol. 38:2344-2347.
2. Blessmann, J., H. Buss, P. A. Nu, B. T. Dinh, Q. T. Ngo, A. L. Van, M. D. Alla, T. F. Jackson, J. I. Ravdin, and E. Tannich. 2002. Real-time PCR for detection and differentiation of Entamoeba histolytica and Entamoeba dispar in fecal samples. J. Clin. Microbiol. 40:4413-4417.
3. Britten, D., S. M. Wilson, R. McNerney, A. H. Moody, P. L. Chiodini, and J. P. Ackers. 1997. An improved colorimetric PCR-based method for detection and differentiation of Entamoeba histolytica and Entamoeba dispar in feces. J. Clin. Microbiol. 35:1108-1111.
4. Crepin, P., L. Aurdy, Y. Rotivel, A. Gacoin, C. Caroff, and H. Bourhy. 1998. Intravitam diagnosis of human rabies by PCR using saliva and cerebrospinal fluid. J. Clin. Microbiol. 36:1117-1121.
5. Haque, R., I. K. M. Ali, and W. A. Petri, Jr. 1999. Prevalence and immune response of Entamoeba histolytica infection in preschool children in Bangladesh. Am. J. Trop. Med. Hyg. 60:1031-1034.
6. Haque, R., I. K. M. Ali, S. Akther, and W. A. Petri, Jr. 1998. Comparison of PCR, isoenzyme analysis, and antigen detection for diagnosis of Entamoeba histolytica infection. J. Clin. Microbiol. 36:449-452.
7. Haque, R., C. D. Huston, M. Hughes, E. Houpt, and W. A. Petri, Jr. 2003. Current concepts: amebiasis. N. Engl. J. Med. 348:1565-1573.
8. Haque, R., N. U. Mollah, I. K. M. Ali, K. Alam, A. Eubanks, D. Lyerly, and W. A. Petri, Jr. 2000. Diagnosis of amebic liver abscess and intestinal infection with the TechLab Entamoeba histolytica II antigen detection and antibody tests. J. Clin. Microbiol. 38:3235-3239.
9. Haque, R., L. M. Neville, P. Hahn, and W. A. Petri, Jr. 1995. Rapid diagnosis of Entamoeba infection by using Entamoeba and Entamoeba histolytica stool antigen detection kits. J. Clin. Microbiol. 33:2558-2561.
10. Haque, R., S. Roy, A. Siddique, U. Mondal, S. M. M. Rahman, D. Mondal, E. Houpt, and W. A. Petri, Jr. 2007. Multiplex real time PCR assay for the diagnosis of Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp. Am. J. Trop. Med. Hyg. 76:713-717.
11. Juniper, K., C. L. Worrell, M. C. Minshew, L. S. Roth, H. Cypert, and R. E. Lloyd. 1972. Serologic diagnosis of amebiasis. Am. J. Trop. Med. Hyg. 21:157-167.
12. Kasper, D. L., E. Braunwald, A. S. Fauci, S. L. Hauser, D. L. Longo, and J. L. Jameson. 2005. Harrison's principles of internal medicine, 16th ed., p. 1215-1216. McGraw Hill, New York, NY.
13. Katzenstein, D., V. Rickerson, and A. Braude. 1982. New concepts of amebic liver abscess derived from hepatic imaging, serodiagnosis, and hepatic enzymes in 67 consecutive cases in San Diego. Medicine (Baltimore) 68:237-246. (Pubitemid 12039758)
14. Khairnar, K., and S. C. Parija. 2008. Detection of E. histolytica DNA in the saliva of amebic liver abscess patients who received prior treatment with metronidazole. J. Health Popul. Nutr. 26:418-425.
15. Khan, U., B. R. Mirdha, J. C. Samantray, and M. P. Sharma. 2006. Detection of Entamoeba histolytica using polymerase chain reaction in pus samples from amebic liver abscess. Indian J. Gastroentrol. 25:55-57.
16. Krupp, I. M., and S. J. Powell. 1971. Comparative study of the antibody response in amebiasis: persistence after successful treatment. Am. J. Trop. Med. Hyg. 20:421-424.
17. Li, C., P. R. Musich, T. Ha, D. A. Ferguson, Jr., N. R. Patel, D. S. Chi, et al. 1995. High prevalence of Helicobacter pylori in saliva demonstrated by a novel PCR assay. J. Clin. Pathol. 48:662-666.
18. Nunez, Y. O., M. A. Fernandez, D. Torres-Nunez, J. A. Silva, I. Montano, J. L. Maestre, et al. 2001. Multiplex polymerase chain reaction amplification and differentiation of Entamoeba histolytica and Entamoeba dispar DNA from stool samples. Am. J. Trop. Med. Hyg. 64:293-297.
19. Parija, S. C., and K. Khairnar. 2007. Detection of excretory Entamoeba histolytica DNA in the urine, and detection of E. histolytica DNA and lectin antigen in the liver abscess pus for the diagnosis of amoebic liver abscess. BMC Microbiol. 7:41.
20. Qvarnstrom, Y. C., C. James, M. Xayavong, B. P. Holloway, G. S. Visvesvera, R. Sriram, and A. J. Silva. 2005. Comparison of real-time PCR protocols for differential laboratory diagnosis of amebiasis. J. Clin. Microbiol. 43:5491-5497.
21. Roy, S., M. Kabir, D. Mondal, I. K. M. Ali, W. A. Petri, Jr., and R. Haque. 2005. Real-time PCR assay for the diagnosis of Entamoeba histolytica infection. J. Clin. Microbiol. 43:2168-2172.
22. Stanley, S. L., Jr. 2003. Amoebiasis. Lancet 361:1025-1034.
23. Stanley, S. L., Jr., T. F. G. H. Jackson, L. Foster, and S. Singh. 1998. Longitudinal study of the antibody response to recombinant E. histolytica antigen in patients with amebic liver abscess. Am. J. Trop. Med. Hyg. 58:414-416.
24. Troll, H., H. Marti, and N. Weiss. 1997. Simple differential detection of Entamoeba histolytica and Entamoeba dispar in fresh stool specimens by sodium acetate-acetic acid formalin concentration and PCR. J. Clin. Microbiol. 35:1701-1705.
25. World Health Organization. 1997. Amoebiasis. Wkly. Epidemiol. Rec. 72:97-100.
26. Zaman, S., J. Khoo, S. W. Ng, R. Ahmed, M. A. Khan, R. Hussain, and V. Zaman. 2000. Direct amplification of Entamoeba histolytica DNA from amoebic liver abscess pus using polymerase chain reaction. Parasitol. Res. 86:724-728.