Loss of dsRNA-based gene silencing in Entamoeba histolytica: Implications for approaches to genetic analysis

Experimental Parasitology

Volumn 119, Issue 2, 2008, Pages 296-300

  MacFarlane, Ryan C ^a   Singh, Upinder ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Ameba; Gene expression; Parasite; RNAi

Indexed keywords

DOUBLE STRANDED RNA; ISOLEUCINE; SERINE; THREONINE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CYTOTOXICITY; ENTAMOEBA HISTOLYTICA; EUKARYOTIC CELL; GENE CONSTRUCT; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE SILENCING; GENETIC ANALYSIS; NONHUMAN; PLASMID; PRIORITY JOURNAL; RNA INTERFERENCE;

ANIMALS; BLOTTING, NORTHERN; DNA PRIMERS; DOWN-REGULATION; ENTAMOEBA HISTOLYTICA; GENE SILENCING; PLASMIDS; POLYMERASE CHAIN REACTION; RNA, DOUBLE-STRANDED; TRANSFECTION;

ENTAMOEBA HISTOLYTICA; EUKARYOTA; PROTOZOA;

EID: 42749096323     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2008.02.001     Document Type: Article

References (20)

1. WHO/PAHO/UNESCO report. 1997. A consultation with experts on amoebiasis. Mexico City, Mexico 28-29 January, 1997. Epidemiology Bulletin 18, 13-14.
2. Abed M., and Ankri S. Molecular characterization of Entamoeba histolytica RNase III and AGO2, two RNA interference hallmark proteins. Experimental Parasitology 110 (2005) 265-269
3. Ali I.K., Ehrenkaufer G.M., Hackney J.A., and Singh U. Growth of the protozoan parasite Entamoeba histolytica in 5-azacytidine has limited effects on parasite gene expression. BMC Genomics 8 (2007) 7
4. Anbar M., Bracha R., Nuchamowitz Y., Li Y., Florentin A., and Mirelman D. Involvement of a short interspersed element in epigenetic transcriptional silencing of the amoebapore gene in Entamoeba histolytica. Eukaryotic Cell 4 (2005) 1775-1784
5. Ankri S., Stolarsky T., and Mirelman D. Antisense inhibition of expression of cysteine proteinases does not affect Entamoeba histolytica cytopathic or haemolytic activity but inhibits phagocytosis. Molecular Microbiology 28 (1998) 777-785
6. Bernes S., Siman-Tov R., and Ankri S. Epigenetic and classical activation of Entamoeba histolytica heat shock protein 100 (EHsp100) expression. FEBS Letters 579 (2005) 6395-6402
7. Bracha R., Nuchamowitz Y., Anbar M., and Mirelman D. Transcriptional silencing of multiple genes in trophozoites of Entamoeba histolytica. PLoS Pathogens 2 (2006) e48
8. Ehrenkaufer G.M., Eichinger D.J., and Singh U. Trichostatin A effects on gene expression in the protozoan parasite Entamoeba histolytica. BMC Genomics 8 (2007) 216
9. Fisher O., Siman-Tov R., and Ankri S. Characterization of cytosine methylated regions and 5-cytosine DNA methyltransferase (Ehmeth) in the protozoan parasite Entamoeba histolytica. Nucleic Acids Research 32 (2004) 287-297
10. Hamann L., Buss H., and Tannich E. Tetracycline-controlled gene expression in Entamoeba histolytica. Molecular and Biochemical Parasitology 84 (1997) 83-91
11. Kaur G., and Lohia A. Inhibition of gene expression with double strand RNA interference in Entamoeba histolytica. Biochemical Biophysics Research Communications 320 (2004) 1118-1122
12. Loftus B., Anderson I., Davies R., Alsmark U.C., Samuelson J., Amedeo P., Roncaglia P., Berriman M., Hirt R.P., Mann B.J., Nozaki T., Suh B., Pop M., Duchene M., Ackers J., Tannich E., Leippe M., Hofer M., Bruchhaus I., Willhoeft U., Bhattacharya A., Chillingworth T., Churcher C., Hance Z., Harris B., Harris D., Jagels K., Moule S., Mungall K., Ormond D., Squares R., Whitehead S., Quail M.A., Rabbinowitsch E., Norbertczak H., Price C., Wang Z., Guillen N., Gilchrist C., Stroup S.E., Bhattacharya S., Lohia A., Foster P.G., Sicheritz-Ponten T., Weber C., Singh U., Mukherjee C., El-Sayed N.M., Petri Jr. W.A., Clark C.G., Embley T.M., Barrell B., Fraser C.M., and Hall N. The genome of the protist parasite Entamoeba histolytica. Nature 433 (2005) 865-868
13. MacFarlane R.C., and Singh U. Identification of an Entamoeba histolytica serine, threonine, isoleucine, rich protein with roles in adhesion and cytotoxicity. Eukaryotic Cell 6 (2007) 2139-2146
14. Mello C.C., and Conte Jr. D. Revealing the world of RNA interference. Nature 431 (2004) 338-342
15. Ramakrishnan G., Vines R.R., Mann B.J., and Petri Jr. W.A. A tetracycline-inducible gene expression system in Entamoeba histolytica. Molecular and Biochemical Parasitology 84 (1997) 93-100
16. Shah P.H., MacFarlane R.C., Bhattacharya D., Matese J.C., Demeter J., Stroup S.E., and Singh U. Comparative genomic hybridizations of Entamoeba strains reveal unique genetic fingerprints that correlate with virulence. Eukaryotic Cell 4 (2005) 504-515
17. Shi H., Chamond N., Tschudi C., and Ullu E. Selection and characterization of RNA interference-deficient trypanosomes impaired in target mRNA degradation. Eukaryotic Cell 3 (2004) 1445-1453
18. Stock R.P., Olvera A., Sanchez R., Saralegui A., Scarfi S., Sanchez-Lopez R., Ramos M.A., Boffa L.C., Benatti U., and Alagon A. Inhibition of gene expression in Entamoeba histolytica with antisense peptide nucleic acid oligomers. Nature Biotechnology 19 (2001) 231-234
19. Vayssie L., Vargas M., Weber C., and Guillen N. Double-stranded RNA mediates homology-dependent gene silencing of gamma-tubulin in the human parasite Entamoeba histolytica. Molecular and Biochemical Parasitology 138 (2004) 21-28
20. Vines R.R., Ramakrishnan G., Rogers J.B., Lockhart L.A., Mann B.J., and Petri Jr. W.A. Regulation of adherence and virulence by the Entamoeba histolytica lectin cytoplasmic domain, which contains a beta2 integrin motif. Molecular Biology of the Cell 9 (1998) 2069-2079