Improvement of the acid stability of Bacillus licheniformis alpha amylase by error-prone PCR

Journal of Applied Microbiology

Volumn 113, Issue 3, 2012, Pages 541-549

  Liu, Y H ^a,b,d   Hu, B ^a,d   Xu, Y J ^a,d   Bo, J X ^a,d   Fan, S ^c,d   Wang, J L ^d   Lu, F P ^a,b,d  

^a BEIJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS   (China)

^b National Engineering Laboratory for Industrial Enzymes   (China)

^c Tianjin Key Laboratory of Industrial Microbiology   (China)

^d TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Acid stability; Bacillus licheniformis alpha amylase; Characterization; Error prone PCR; Protein structure

Indexed keywords

AMYLASES; BACTERIOLOGY; ERRORS; HYDROGEN BONDS; STABILITY;

ACID CONDITIONS; ACID STABILITY; ACIDIC CONDITIONS; BACILLUS LICHENIFORMIS ALPHA AMYLASE; CATALYTIC MECHANISMS; CHARACTERIZATION; DIRECT EVOLUTIONS; ERROR PRONE PCR; PROTEINS STRUCTURES; WILD TYPES;

POLYMERASE CHAIN REACTION;

ACID; AMYLASE; BACTERIAL ENZYME; HISTONE; THREONINE;

ACID; AMINO ACID; BACTERIUM; ENZYME ACTIVITY; HYDROPHOBICITY; MUTATION; PH; POLYMERASE CHAIN REACTION; PROTEIN; THREE-DIMENSIONAL MODELING;

ARTICLE; BACILLUS LICHENIFORMIS; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE MUTATION; MUTAGENESIS; NONHUMAN; PH; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; THERMOSTABILITY;

BACILLUS LICHENIFORMIS;

EID: 84865289946     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2012.05359.x     Document Type: Article

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