Negative selection assay based on stimulation of T cell receptor transgenic thymocytes with peptide-MHC tetramers

PLoS ONE

Volumn 7, Issue 8, 2012, Pages

  Rybakin, Vasily ^a   Gascoigne, Nicholas R J ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CASPASE 3; GLUTAMYLISOLEUCYLISOLEUCYLASPARAGYLPHENYLALANYLGLUTAMYLLYSYLLEUCINE; SERYLALANYLISOLEUCYLASPARAGYLPHENYLALANYLGLUTAMYLLYSYLLEUCINE; SERYLISOLEUCYLISOLEUCYLASPARAGYLPHENYLALANYLGLUTAMYLGLUTAMINYLLEUCINE; SERYLISOLEUCYLISOLEUCYLGLYCYLPHENYLALANYLGLUTAMYLLYSYLLEUCINE; T LYMPHOCYTE RECEPTOR; TETRAMER; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; APOPTOSIS; ARTICLE; CELL DEATH; CELL STIMULATION; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME INDUCTION; HIGH THROUGHPUT SCREENING; IMMUNOLOGICAL TOLERANCE; IN VITRO STUDY; INTRACELLULAR SIGNALING; MAJOR HISTOCOMPATIBILITY COMPLEX; MOUSE; NONHUMAN; PROTEIN CROSS LINKING; PURIFYING SELECTION; THYMOCYTE;

ANIMALS; APOPTOSIS; CASPASE 3; CELL DIFFERENTIATION; CLONAL DELETION; HISTOCOMPATIBILITY ANTIGENS; HUMANS; LYMPHOCYTE ACTIVATION; MICE; MICE, TRANSGENIC; PEPTIDES; RECEPTORS, ANTIGEN, T-CELL; THYMOCYTES;

EID: 84865008910     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0043191     Document Type: Article

References (33)

1. Starr TK, Jameson SC, Hogquist KA, ((2003)) Positive and negative selection of T cells. Annu Rev Immunol 21:: 139--176.
2. Palmer E, ((2003)) Negative selection-clearing out the bad apples from the T-cell repertoire. Nat Rev Immunol 3:: 383--391.
3. Gascoigne NR, Palmer E, ((2011)) Signaling in thymic selection. Curr Opin Immunol 23:: 207--212.
4. Anderson MS, Su MA, ((2011)) Aire and T cell development. Curr Opin Immunol 23:: 198--206.
5. Staton TL, Lazarevic V, Jones DC, Lanser AJ, Takagi T, et al. ((2011)) Dampening of death pathways by schnurri-2 is essential for T-cell development. Nature 472:: 105--109.
6. Punt JA, Havran W, Abe R, Sarin A, Singer A, ((1997)) T cell receptor (TCR)-induced death of immature CD4+CD8+ thymocytes by two distinct mechanisms differing in their requirement for CD28 costimulation: implications for negative selection in the thymus. J Exp Med 186:: 1911--1922.
7. Punt JA, Osborne BA, Takahama Y, Sharrow SO, Singer A, ((1994)) Negative selection of CD4+CD8+ thymocytes by T cell receptor-induced apoptosis requires a costimulatory signal that can be provided by CD28. J Exp Med 179:: 709--713.
8. Hogquist KA, Jameson SC, Heath WR, Howard JL, Bevan MJ, et al. ((1994)) T cell receptor antagonist peptides induce positive selection. Cell 76:: 17--27.
9. Alam SM, Travers PJ, Wung JL, Nasholds W, Redpath S, et al. ((1996)) T cell receptor affinity and thymocyte positive selection. Nature 381:: 616--620.
10. Daniels MA, Teixeiro E, Gill J, Hausmann B, Roubaty D, et al. ((2006)) Thymic selection threshold defined by compartmentalization of Ras/MAPK signalling. Nature 444:: 724--729.
11. Hogquist KA, Tomlinson AJ, Kieper WC, McGargill MA, Hart MC, et al. ((1997)) Identification of a naturally occurring ligand for positive selection. Immunity 6:: 389--399.
12. Page DM, Kane LP, Allison JP, Hedrick SM, ((1993)) Two signals are required for negative selection of CD4+CD8+ thymocytes. J Immunol 151:: 1868--1880.
13. Swat W, Ignatowicz L, von Boehmer H, Kisielow P, ((1991)) Clonal deletion of immature CD4+8+ thymocytes in suspension culture by extrathymic antigen-presenting cells. Nature 351:: 150--153.
14. Mingueneau M, Jiang W, Feuerer M, Mathis D, Benoist C, ((2012)) Thymic negative selection is functional in NOD mice. J Exp Med 209:: 623--637.
15. Anderson G, Jenkinson EJ (2007) Fetal thymus organ culture. CSH Protoc 2007: pdb prot4808.
16. Hogquist KA, Gavin MA, Bevan MJ, ((1993)) Positive selection of CD8+ T cells induced by major histocompatibility complex binding peptides in fetal thymic organ culture. J Exp Med 177:: 1469--1473.
17. Brunelle JK, Zhang B, ((2010)) Apoptosis assays for quantifying the bioactivity of anticancer drug products. Drug Resist Updat 13:: 172--179.
18. Bogojevic D, Chamberlain MD, Barbulovic-Nad I, Wheeler AR, ((2012)) A digital microfluidic method for multiplexed cell-based apoptosis assays. Lab Chip 12:: 627--634.
19. Alam A, Braun MY, Hartgers F, Lesage S, Cohen L, et al. ((1997)) Specific activation of the cysteine protease CPP32 during the negative selection of T cells in the thymus. J Exp Med 186:: 1503--1512.
20. Jiang D, Zheng L, Lenardo MJ, ((1999)) Caspases in T-cell receptor-induced thymocyte apoptosis. Cell Death Differ 6:: 402--411.
21. Ohta T, Kinoshita T, Naito M, Nozaki T, Masutani M, et al. ((1997)) Requirement of the caspase-3/CPP32 protease cascade for apoptotic death following cytokine deprivation in hematopoietic cells. J Biol Chem 272:: 23111--23116.
22. Murakami K, Liadis N, Sarmiento J, Elford AR, Woo M, et al. ((2010)) Caspase 3 is not essential for the induction of anergy or multiple pathways of CD8+ T-cell death. Eur J Immunol 40:: 3372--3377.
23. Einarsdottir T, Lockhart E, Flynn JL, ((2009)) Cytotoxicity and secretion of gamma interferon are carried out by distinct CD8 T cells during Mycobacterium tuberculosis infection. Infect Immun 77:: 4621--4630.
24. Feng B, Yao PM, Li Y, Devlin CM, Zhang D, et al. ((2003)) The endoplasmic reticulum is the site of cholesterol-induced cytotoxicity in macrophages. Nat Cell Biol 5:: 781--792.
25. Garza AS, Miller AL, Johnson BH, Thompson EB, ((2009)) Converting cell lines representing hematological malignancies from glucocorticoid-resistant to glucocorticoid-sensitive: signaling pathway interactions. Leuk Res 33:: 717--727.
26. Lawson BR, Baccala R, Song J, Croft M, Kono DH, et al. ((2004)) Deficiency of the cyclin kinase inhibitor p21(WAF-1/CIP-1) promotes apoptosis of activated/memory T cells and inhibits spontaneous systemic autoimmunity. J Exp Med 199:: 547--557.
27. Surh CD, Sprent J, ((1994)) T-cell apoptosis detected in situ during positive and negative selection in the thymus. Nature 372:: 100--103.
28. Veis DJ, Sorenson CM, Shutter JR, Korsmeyer SJ, ((1993)) Bcl-2-deficient mice demonstrate fulminant lymphoid apoptosis, polycystic kidneys, and hypopigmented hair. Cell 75:: 229--240.
29. Alam SM, Davies GM, Lin CM, Zal T, Nasholds W, et al. ((1999)) Qualitative and quantitative differences in T cell receptor binding of agonist and antagonist ligands. Immunity 10:: 227--237.
30. Le Meur N, Rossini A, Gasparetto M, Smith C, Brinkman RR, et al. ((2007)) Data quality assessment of ungated flow cytometry data in high throughput experiments. Cytometry A 71:: 393--403.
31. Schlegel J, Peters I, Orrenius S, Miller DK, Thornberry NA, et al. ((1996)) CPP32/apopain is a key interleukin 1 beta converting enzyme-like protease involved in Fas-mediated apoptosis. J Biol Chem 271:: 1841--1844.
32. Gamen S, Marzo I, Anel A, Pineiro A, Naval J, ((1996)) CPP32 inhibition prevents Fas-induced ceramide generation and apoptosis in human cells. FEBS Lett 390:: 232--237.
33. Fu G, Gascoigne NRJ, ((2009)) Multiplexed labeling of samples with cell tracking dyes facilitates rapid and accurate internally controlled calcium flux measurement by flow cytometry. J Immunol Methods 350:: 194--199.