Multiplexed labeling of samples with cell tracking dyes facilitates rapid and accurate internally controlled calcium flux measurement by flow cytometry

Journal of Immunological Methods

Volumn 350, Issue 1-2, 2009, Pages 194-199

  Fu, Guo ^a   Gascoigne, Nicholas R J ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Calcium flux; CFSE; Cy5; Lymphocyte activation

Indexed keywords

CALCIUM ION; DYE;

ACCURACY; ARTICLE; CALCIUM TRANSPORT; CELL MIGRATION; CELL PROLIFERATION; CONTROLLED STUDY; FLOW CYTOMETRY; LYMPHOCYTE ACTIVATION; LYMPHOCYTE SUBPOPULATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; THYMOCYTE;

ANIMALS; CALCIUM; CARBOCYANINES; FLOW CYTOMETRY; FLUORESCEINS; MICE; MICE, KNOCKOUT; SUCCINIMIDES;

EID: 70349801932     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2009.07.009     Document Type: Article

References (10)

1. Adams C.L., Rush C.M., Smith K.M., and Garside P. Tracking antigen-specific lymphocytes in vivo. Methods Mol. Biol. 239 (2004) 133
2. Feske S. Calcium signalling in lymphocyte activation and disease. Nat. Rev., Immunol. 7 (2007) 690
3. Fu G., Vallee S., Rybakin V., McGuire M.V., Ampudia J., Brockmeyer C., Salek M., Fallen P.R., Hoerter J.A.H., Munshi A., Huang Y.H., Hu J., Fox H.S., Sauer K., Acuto O., and Gascoigne N.R.J. Themis controls thymocyte selection through regulation of T cell antigen receptor-mediated signaling. Nat. Immunol. 10 (2009) 848
4. Haraldsson M.K., Louis-Dit-Sully C.A., Lawson B.R., Sternik G., Santiago-Raber M.L., Gascoigne N.R.J., Theofilopoulos A.N., and Kono D.H. The lupus-related Lmb3 locus contains a disease-suppressing Coronin-1A gene mutation. Immunity 28 (2008) 40 (PMCID: PMC2274909)
5. Hawkins E.D., Hommel M., Turner M.L., Battye F.L., Markham J.F., and Hodgkin P.D. Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data. Nat. Protoc. 2 (2007) 2057
6. Liao X.C., and Littman D.R. Altered T cell receptor signaling and disrupted T cell development in mice lacking Itk. Immunity 3 (1995) 757
7. Liu K.Q., Bunnell S.C., Gurniak C.B., and Berg L.J. T cell receptor-initiated calcium release is uncoupled from capacitative calcium entry in Itk-deficient T cells. J. Exp. Med. 187 (1998) 1721
8. Nelemans A. Measurement of [Ca2+] in cell suspensions using indo-1. Methods Mol. Biol. 312 (2006) 47
9. Quah B.J., Warren H.S., and Parish C.R. Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester. Nat. Protoc. 2 (2007) 2049
10. Simpson A.W. Fluorescent measurement of [Ca2+]c: basic practical considerations. Methods Mol. Biol. 312 (2006) 3