Directed evolution of cutinase using in vitro compartmentalization

Biotechnology and Bioprocess Engineering

Volumn 17, Issue 3, 2012, Pages 500-505

  Hwang, Bum Yeol ^a  

^a University at California Berkeley   (United States)

Author keywords

Cutinase; Directed evolution; Flurbiprofen; In vitro compartmentalization

Indexed keywords

CUTINASE; DIESTERS; DIRECTED EVOLUTION; E. COLI; FLUORESCENCE ACTIVATED CELL SORTING; FLUORESCENT PRODUCTS; FLURBIPROFEN; FUSARIUM SOLANI; HIGH-THROUGHPUT SCREENING; IN-VITRO; MICRO-COMPARTMENTS; OIL/WATER EMULSION; SCREENING TECHNIQUES; WILD TYPES;

EMULSIFICATION; ESCHERICHIA COLI; FLUORESCENCE;

FLUORESCENT SCREENS;

CUTINASE; FLURBIPROFEN;

ARTICLE; BACTERIAL CELL; CELL COMPARTMENTALIZATION; EMULSION; ENZYME ACTIVITY; ENZYME STRUCTURE; ESCHERICHIA COLI; FLUORESCENCE ACTIVATED CELL SORTING; FUSARIUM SOLANI; HIGH THROUGHPUT SCREENING; IN VITRO STUDY; MOLECULAR EVOLUTION; NONHUMAN; PROTEIN EXPRESSION; PROTEIN FUNCTION; WILD TYPE;

FUSARIUM SOLANI;

EID: 84864718521     PISSN: 12268372     EISSN: 19763816     Source Type: Journal    
DOI: 10.1007/s12257-012-0026-1     Document Type: Article

References (23)

1. Stemmer, W. P. (1994) Rapid evolution of a protein in vitro by DNA shuffling. Nature 370: 389-391.
2. Kuchner, O. and F. H. Arnold (1997) Directed evolution of enzyme catalysts. Trends Biotechnol. 15: 523-530.
3. Jaeger, K. E., T. Eggert, A. Eipper, and M. T. Reetz (2001) Directed evolution and the creation of enenatioselective biocata-lysts. Appl. Microbiol. Biotechnol. 55: 519-530.
4. Bornscheuer, U. T. and M. Pohl (2001) Improved biocatalysts by directed evolution and rational protein design. Curr. Opin. Chem. Biol. 5: 137-143.
5. st ed., Humana press Inc. NJ, USA.
6. van Loo, B., J. H. Spelberg, J. Kingma, T. Sonke, M. G. Wub-bolts, and D. B. Janssen (2004) Directed evolution of epoxide hydrolase from A. radiobacter toward higher enantioselectivity by error-prone PCR and DNA shuffling. Chem. Biol. 11: 981-990.
7. Hwang, B. -Y. and B. -G. Kim (2004) High-throughput screening method for the identification of active and enantioselective ω-transaminases. Enz. Microbiol. Technol. 34: 429-436.
8. Chen, G., A. Hayhurst, J. G. Thomas, B. R. Harvey, B. L. Iverson, and G. Georgiou (2001) Isolation of high-affinity ligand-binding proteins by periplasmic expression with cytometric screening (PECS). Nat. Biotechnol. 19: 537-542.
9. Becker, S., H. -U. Schmoldt, T. M. Adams, S. Wilhelm, and H. Kolmar (2004) Ultra-high throughput screening based on cell-surface display and fluorescence-activated cell sorting for the identification of novel biocatalysts. Curr. Opin. Biotechnol. 15: 323-329.
10. Varadarajan, N., S. Rodriguez, B. -Y. Hwang, G. Georgiou, and B. L. Iverson (2008) Highly active and selective endopeptidases with programmed substrate specificities. Nat. Chem. Biol. 4: 290-294.
11. th ed., Wiley-Liss, NY, USA.
12. Olsen, M. J., D. Stephens, D. Griffiths, P. Daugherty, G. Geor-giou, and B. L. Iverson (2000) Function-based isolation of novel enzymes from a large library. Nat. Biotechnol. 18: 1071-1074.
13. Varadarajan, N., J. Gam, M. J. Olsen, G. Georgiou, and B. L. Iverson (2005) Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity. PNAS 102: 6855-6860.
14. Griswold, K. E., Y. Kawarasaki, N. Ghoneim, S. J. Benkovic, B. L. Iverson, and G. Georgiou (2005) Evolution of highly active enzymes by homology-independent recombination. PNAS 120: 10082-10087.
15. Levy, M., K. E. Griswold, and A. D. Ellington (2005) Direct selection of trans-acting ligase ribozymes by in vitro compart-mentalization. RNA 11: 1555-1562.
16. Griffiths, A. D. and D. S. Tawfik (2003) Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentaliza-tion. EMBO J. 22: 24-35.
17. Aharoni, A., G. Amital, K. Bernath, S. Magdassi, and D. S. Taw-fik (2005) High-throughput screening of enzyme libraries: Thi-olactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments. Chem. Biol. 12: 1281-1289.
18. Pio, T. F. and G. A. Macedo (2009) Cutinases: Properties and industrial applications. Adv. Appl. Microbiol. 66: 77-95.
19. Longhi, S., M. Czjzek, V. Lamzin, A. Nicolas, and C. Cambillau (1997) Atomic resolution (1.0 A) crystal structure of Fusarium solani cutinase: stereochemical analysis. J. Mol. Biol. 268: 779-799.
20. Griswold, K. E., N. A. Mahmood, B. L. Iverson, and G. Georgiou (2003) Effects of codon usage versus putative 5'-mRNA structure on the expression of Fusarium solani cutinase in the Escherichia coli cytoplasm. Protein Expr. Purif. 27: 134-142.
21. Ettinger, W. F., S. K. Thukral, and P. E. Kolattukudy (1987) Structure of cutinase gene, cDNA, and the derived amino acid sequence from phytopathogenic fungi. Biochem. 26: 7883-7892.
22. Quann, E. J., F. Khwaja, K. H. Zavitz, and D. Djakiew (2007) The aryl propionic acid R-flurbiprofen selectively induces p75NTR-dependent decreased survival of prostate tumor cells. Cancer Res. 67: 3254-3262.
23. Geerts, H. (2007) Drug evaluation: (R)-flurbiprofen-an enanti-omer of flurbiprofen for the treatment of Alzheimer's disease. IDrugs 10: 121-133.