EPR detection of two protein-associated ubiquinone components (SQ Nf and SQNs) in the membrane in situ and in proteoliposomes of isolated bovine heart complex i

Biochimica et Biophysica Acta - Bioenergetics

Volumn 1817, Issue 10, 2012, Pages 1803-1809

  Ohnishi, Tomoko ^a   Ohnishi, S Tsuyoshi ^a   Shinzawa Itoh, Kyoko ^b   Yoshikawa, Shinya ^b   Weber, Ralph T ^c  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b UNIVERSITY OF HYOGO   (Japan)

^c BRUKER BIOSPIN CORPORATION   (United States)

Author keywords

Bovine heart complex I; EPR; Membrane in situ; Proteoliposome; Proton pumping mechanism; SQ Nf and SQ Ns

Indexed keywords

BACTERIAL PROTEIN; IRON; PROTEOLIPOSOME; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE (UBIQUINONE); ROTENONE; SEMIQUINONE; SULFUR; UBIQUINONE;

CONFERENCE PAPER; CRYSTAL STRUCTURE; ELECTRON SPIN RESONANCE; ENERGY TRANSFER; MEMBRANE; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTON MOTIVE FORCE; REDUCTION; RESPIRATION CONTROL; SUBMITOCHONDRIAL PARTICLE;

ANIMALS; BENZOQUINONES; CATTLE; CRYSTALLOGRAPHY, X-RAY; ELECTRON SPIN RESONANCE SPECTROSCOPY; ELECTRON TRANSPORT COMPLEX I; MITOCHONDRIA, HEART; MITOCHONDRIAL PROTEINS; MODELS, MOLECULAR; MUSCLE PROTEINS; OXYGEN CONSUMPTION; PROTON-MOTIVE FORCE;

BACTERIA (MICROORGANISMS); BOVINAE;

EID: 84864701195     PISSN: 00052728     EISSN: 18792650     Source Type: Journal    
DOI: 10.1016/j.bbabio.2012.03.032     Document Type: Conference Paper

References (53)

1. Z. Zhu, and M.R. Gunner Energetics of quinone-dependent electron and proton transfers in Rhodobacter sphaeroides photosynthetic reaction centers Biochemistry 44 2005 82 96
2. C.A. Wraight Proton and electron transfer in the acceptor quinone complex of photosynthetic reaction centers from Rhodobacter spheroides Front. Biosci. 9 2004 309 333
3. M.Y. Okamura, M.L. Paddock, M.S. Graige, and G. Feher Proton and electron transfer in bacterial reaction centers Biochim. Biophys. Acta 1458 2000 148 163
4. P. Fromme, P. Jordan, and N. Krauss Structure of photosystem I Biochim. Biophys. Acta 1507 2001 5 31
5. P. Mitchell The protonmotive Q cycle: a general formulation FEBS Lett. 59 1975 137 139
6. C. Lange, and C. Hunte Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c Proc. Natl. Acad. Sci. U. S. A. 99 2002 2800 2805
7. M. Sato-Watanabe, S. Itoh, T. Mogi, K. Matsuura, H. Miyoshi, and Y. Anraku Stabilization of a semiquinone radical at the high-affinity quinone-binding site (QH) of the Escherichia coli bo-type ubiquinol oxidase FEBS Lett. 374 1995 265 269
8. L.L. Yap, M.T. Lin, H. Ouyang, R.I. Samoilova, S.A. Dikanov, and R.B. Gennis The quinone-binding sites of the cytochrome bo3 ubiquinol oxidase from Escherichia coli Biochim. Biophys. Acta 1797 2010 1924 1932
9. L. Murray, R.H. Pires, S.F. Hastings, and W.J. Ingledew Models for structure and function in quinone-binding sites: the Escherichia coli quinol oxidase, cytochrome bo3 Biochem. Soc. Trans. 27 1999 581 585
10. P. Mitchell Possible molecular mechanisms of the protonmotive function of cytochrome systems J. Theor. Biol. 62 1976 327 367
11. J.C. Salerno, and T. Ohnishi Studies on the stabilized ubisemiquinone species in the succinate-cytochrome c reductase segment of the intact mitochondrial membrane system Biochem. J. 192 1980 769 781
12. T. Ohnishi, C.C. Moser, C.C. Page, P.L. Dutton, and T. Yano Simple redox-linked proton-transfer design: new insights from structures of quinol-fumarate reductase Struct. Fold Des. 8 2000 R23 R32
13. A.R. Crofts, and E.A. Berry Structure and function of the cytochrome bc1 complex of mitochondria and photosynthetic bacteria Curr. Opin. Struct. Biol. 8 1998 501 509
14. T. Ohnishi, J.C. Salerno, and H. Blum Ubisemiquinones in electron-transfer systems of mitochondria B.L. Trumpower, Function of Quinones in Energy Conserving Systems 1982 Academic Press New York 247 261
15. J. Carroll, I.M. Fearnley, J.M. Skehel, R.J. Shannon, J. Hirst, and J.E. Walker Bovine complex I is a complex of 45 different subunits J. Biol. Chem. 281 2006 32724 32727
16. M. Wirkstrom Two protons are pumped from the mitochondrial matrix per electron transferred between NADH and ubiquinone FEBS Lett. 169 1984 300 304
17. 1-ATP synthase FEBS Lett. 448 1999 123 126
18. T. Ohnishi, J.E. Johnson Jr., T. Yano, R. Lobrutto, and W.R. Widger Thermodynamic and EPR studies of slowly relaxing ubisemiquinone species in the isolated bovine heart complex I FEBS Lett. 579 2005 500 506
19. T. Ohnishi Iron-sulfur clusters/semiquinones in complex I Biochim. Biophys. Acta 1364 1998 186 206
20. S. Magnitsky, L. Toulokhonova, T. Yano, V.D. Sled, C. Hagerhall, V.G. Grivennikova, D.S. Burbaev, A.D. Vinogradov, and T. Ohnishi EPR characterization of ubisemiquinones and iron-sulfur cluster N2, central components of the energy coupling in the NADH-ubiquinone oxidoreductase (complex I) in situ J. Bioenerg. Biomembr. 34 2002 193 208
21. Nf) and the interaction with cluster N2: new insight into the energy-coupled electron transfer in complex I Biochemistry 44 2005 1744 1754
22. A.D. Vinogradov Catalytic properties of the mitochondrial NADH-ubiquinone oxidoreductase (complex I) and the pseudo-reversible active/inactive enzyme transition Biochim. Biophys. Acta 1364 1998 169 185
23. J.R. Bowyer, and T. Ohnishi EPR spectroscopy in the study of ubisemiquinones in redox chains G. Lenaz, Coenzyme Q 1985 John Wiley & Sons New York 409 432
24. T. Ohnishi, S. Magnitsky, L. Toulokhonova, T. Yano, D.S. Burbaev, and A. Vinogradov EPR studies of the possible binding sites of the cluster N2, semiquinone, and specific inhibitors of the NADH:quinone oxidoreductase (complex I) Biochem. Soc. Trans. 27 1999 26 30
25. P.L. Dutton, C.C. Moser, V.D. Sled, F. Daldal, and T. Ohnishi A reductant-induced oxidation mechanism for Complex I Biochim. Biophys. Acta Bioenerg. 1364 1998 245 257
26. U. Brandt Proton translocation in the respiratory chain involving ubiquinone - a hypothetical semiquinone switch mechanism for complex I Biofactors 9 1999 95 101
27. J.C. Salerno, H. Blum, and T. Ohnishi The orientation of iron-sulfur clusters and a spin-coupled ubiquinone pair in the mitochondrial membrane Biochim. Biophys. Acta 547 1979 27 281
28. T. Ohnishi, and J.C. Salerno Conformation-driven and semiquinone-gated proton-pump mechanism in the NADH-ubiquinone oxidoreductase (complex I) FEBS Lett. 579 2005 4555 4561
29. S.T. Ohnishi, J.C. Salerno, and T. Ohnishi Possible roles of two quinone molecules in direct and indirect proton pumps of bovine heart NADH-quinone oxidoreductase (complex I) Biochim. Biophys. Acta 1797 2010 1891 1893
30. C. Mathiesen, and C. Hagerhall Transmembrane topology of the NuoL, M and N subunits of NADH:quinone oxidoreductase and their homologues among membrane-bound hydrogenases and bona fide antiporters Biochim. Biophys. Acta 1556 2002 121 132
31. T. Ohnishi, E. Nakamaru-Ogiso, and S.T. Ohnishi A new hypothesis on the simultaneous direct and indirect proton pump mechanisms in NADH-quinone oxidoreductase (complex I) FEBS Lett. 584 2010 4131 4137
32. B. Amarneh, and S.B. Vik Mutagenesis of subunit N of the Escherichia coli complex I. Identification of the initiation codon and the sensitivity of mutants to decylubiquinone Biochemistry 42 2003 4800 4808
33. E. Nakamaru-Ogiso, H. Han, A. Matsuno-Yagi, E. Keinan, S.C. Sinha, T. Yagi, and T. Ohnishi The ND2 subunit is labeled by a photoaffinity analogue of asimicin, a potent complex I inhibitor FEBS Lett. 584 2010 883 888
34. T. Friedrich The NADH:ubiquinone oxidoreductase (complex I) from Escherichia coli Biochim. Biophys. Acta 1364 1998 134 146
35. L.A. Sazanov, and P. Hinchliffe Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus Science 311 2006 1430 1436
36. R.G. Efremov, R. Baradaran, and L.A. Sazanov The architecture of respiratory complex I Nature 465 2010 441 445
37. R. Kikuno, and T. Miyata Sequence homologies among mitochondrial DNA-coded URF2, URF4 and URF5 FEBS Lett. 189 1985 85 88
38. M. Kitada, M. Hashimoto, T. Kudo, and K. Horikoshi Properties of two different Na+/H+ antiport systems in alkaliphilic Bacillus sp. strain C-125 J. Bacteriol. 176 1994 6464 6469
39. M. Morino, S. Natsui, T. Ono, T.H. Swartz, T.A. Krulwich, and M. Ito Single site mutations in the hetero-oligomeric Mrp antiporter from alkaliphilic Bacillus pseudofirmus OF4 that affect Na+/H+ antiport activity, sodium exclusion, individual Mrp protein levels, or Mrp complex formation J. Biol. Chem. 285 2010 30942 30950
40. T. Ohnishi Structural biology: piston drives a proton pump Nature 465 2010 428 429
41. N. Fisher, and P.R. Rich A motif for quinone binding sites in respiratory and photosynthetic systems J. Mol. Biol. 296 2000 1153 1162
42. J.A. Birrell, and J. Hirst Truncation of subunit ND2 disrupts the threefold symmetry of the antiporter-like subunits in complex I from higher metazoans FEBS Lett. 584 2010 4247 4252
43. F.G. Earley, S.D. Patel, I. Ragan, and G. Attardi Photolabelling of a mitochondrially encoded subunit of NADH dehydrogenase with [3H]dihydrorotenone FEBS Lett. 219 1987 108 112
44. D.C. Wallace Diseases of the mitochondrial DNA Annu. Rev. Biochem. 61 1992 1175 1212
45. D.C. Wallace A mitochondrial paradigm of metabolic and degenerative diseases, aging, and cancer: a dawn for evolutionary medicine Annu. Rev. Genet. 39 2005 359 407
46. J. Smeitink, L. van den Heuvel, and S. DiMauro The genetics and pathology of oxidative phosphorylation Nat. Rev. Genet. 2 2001 342 352
47. M. Murai, K. Sekiguchi, T. Nishioka, and H. Miyoshi Characterization of the inhibitor binding site in mitochondrial NADH-ubiquinone oxidoreductase by photoaffinity labeling using a quinazoline-type inhibitor Biochemistry 48 2009 688 698
48. M. Murai, Y. Mashimo, J. Hirst, and H. Miyoshi Exploring interactions between the 49 kDa and ND1 subunits in mitochondrial NADH-ubiquinone oxidoreductase (complex I) by photoaffinity labeling Biochemistry 50 2011 6901 6908
49. C. Hunte, V. Zickermann, and U. Brandt Functional modules and structural basis of conformational coupling in mitochondrial complex I Science 329 2010 448 451
50. S. Steimle, C. Bajzath, K. Dorner, M. Schulte, V. Bothe, and T. Friedrich Role of subunit NuoL for proton translocation by respiratory complex I Biochemistry 50 2011 3386 3393
51. S. Drose, S. Krack, L. Sokolova, K. Zwicker, H.D. Barth, N. Morgner, H. Heide, M. Steger, E. Nubel, V. Zickermann, S. Kerscher, B. Brutschy, M. Radermacher, and U. Brandt Functional dissection of the proton pumping modules of mitochondrial complex I PLoS Biol. 9 2011 e1001128
52. U. Brandt A two-state stabilization-change mechanism for proton-pumping complex I Biochim. Biophys. Acta 1807 2011 1364 1369
53. T. Ohnishi, S.T. Ohnishi, K. Shinzawa-Ito, and S. Yoshikawa Functional role of coenzyme Q in the energy coupling of NADH-CoQ oxidoreductase (Complex I): stabilization of the semiquinone state with the application of inside-positive membrane potential to proteoliposomes Biofactors 32 2008 13 22