Characterization of the inhibitor binding site in mitochondrial NADH-ubiquinone oxidoreductase by photoaffinity labeling using a quinazoline-type inhibitor

Biochemistry

Volumn 48, Issue 4, 2009, Pages 688-698

  Murai, Masatoshi ^a   Sekiguchi, Koji ^a   Nishioka, Takaaki ^b   Miyoshi, Hideto ^a  

^a KYOTO UNIVERSITY   (Japan)

^b KEIO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

3-HYDROXYBUTYRATE DEHYDROGENASE; ADP/ATP CARRIERS; AMINO ACID RESIDUES; AZIDO GROUPS; BINDING DOMAINS; BOVINE HEARTS; IMMUNOBLOTTING; MITOCHONDRIAL COMPLEXES; MITOCHONDRIAL NADH; N-TERMINAL SEQUENCING; OXIDOREDUCTASE; PARTIAL PROTEOLYSIS; PEPTIDE MASS FINGERPRINTING; PHOTO-CROSS-LINKED; PHOTOAFFINITY LABELING; QUINAZOLINE; SUBMITOCHONDRIAL PARTICLES; UBIQUINONE;

AMINATION; AMINES; AMINO ACIDS; BINDING ENERGY; ENZYMES; LABELING; ORGANIC ACIDS; PROTEINS;

BINDING SITES;

6 AZIDO 4 (4 IODOPHENETHYLAMINO)QUINAZOLINE I 125; ACETOGENIN; ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; AMINO ACID; ARGININE; ASPARTIC ACID; AZIDE; AZIDOQUINAZOLINE; BACTERIAL ENZYME; ENZYME INHIBITOR; FENPYROXIMATE; GLUTAMINE; HYDROXYBUTYRATE DEHYDROGENASE; IODINE 125; LYSYLENDOPEPTIDASE; QUINAZOLINE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE (UBIQUINONE); THREONINE; TRYPSIN; UNCLASSIFIED DRUG; V8 PROTEASE; QUINAZOLINE DERIVATIVE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANALYTIC METHOD; ANIMAL TISSUE; ARTICLE; BINDING SITE; COW; CROSS LINKING; DRUG STRUCTURE; ENZYME STRUCTURE; ENZYME SUBUNIT; HEART; IMMUNOBLOTTING; ISOTOPE LABELING; MITOCHONDRION; NONHUMAN; NUCLEOTIDE SEQUENCE; PEPTIDE MASS FINGERPRINTING; PHOTOAFFINITY LABELING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; ANIMAL; CATTLE; CHEMISTRY; COMPARATIVE STUDY; DRUG ANTAGONISM; ENZYMOLOGY; GENETICS; HEART MITOCHONDRION; METABOLISM; MOLECULAR GENETICS; PHYSIOLOGY; PROTEIN TERTIARY STRUCTURE; X RAY CRYSTALLOGRAPHY;

BOVINAE;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CATTLE; CRYSTALLOGRAPHY, X-RAY; ELECTRON TRANSPORT COMPLEX I; MITOCHONDRIA, HEART; MOLECULAR SEQUENCE DATA; PHOTOAFFINITY LABELS; PROTEIN STRUCTURE, TERTIARY; QUINAZOLINES;

EID: 60749121178     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi8019977     Document Type: Article

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