Expression and characterization of a novel metagenome-derived cellulase Exo2b and its application to improve cellulase activity in Trichoderma reesei

Applied Microbiology and Biotechnology

Volumn 96, Issue 4, 2012, Pages 951-962

  Geng, Alei ^a   Zou, Gen ^a   Yan, Xing ^a   Wang, Qianfu ^a   Zhang, Jun ^a   Liu, Fanghua ^a   Zhu, Baoli ^b   Zhou, Zhihua ^a  

^a Shanghai Jiao Tong University Affiliated Sixth People s Hospital   (China)

^b INSTITUTE OF MICROBIOLOGY   (China)

Author keywords

Cellulase; Fungi; Gene fusion; Metagenome; Pretreated corn stover

Indexed keywords

BIOGAS DIGESTERS; CARBOXY METHYLCELLULOSE; CATALYTIC DOMAINS; CELLULASE; CELLULASE ACTIVITY; CELLULASE GENES; CORN STOVER; FILTER PAPER ACTIVITY; FRAGMENT ENCODING; FUSED GENES; GENE FUSION; GLUCOSE CONCENTRATION; HYDROLYSIS PRODUCTS; METAGENOMES; METAGENOMIC LIBRARIES; PARENT STRAIN; POTENTIAL APPLICATIONS; RESIDUAL ACTIVITY; RICE STRAWS; SECRETED PROTEIN; SPECIFIC ACTIVITY; STABLE ACTIVITY; T. REESEI; TRICHODERMA REESEI; WIDE PH RANGE;

AMINO ACIDS; BIOGAS; CLONING; ESCHERICHIA COLI; FUNGI; GENE ENCODING; GLUCOSE; HYDROLYSIS; THIN LAYER CHROMATOGRAPHY;

RECOMBINANT PROTEINS;

CARBOXYMETHYLCELLULOSE; CELLULASE; CYSTEINE; GLUCOSE; MESSENGER RNA; OLIGOSACCHARIDE; PROTEIN EXO2B; UNCLASSIFIED DRUG;

CATALYSIS; CELLULOSE; COLIFORM BACTERIUM; ENZYME ACTIVITY; FRAGMENTATION; FUNGUS; GENE EXPRESSION; GENOMICS; HYDROLYSIS; MAIZE; PH; PROTEIN; RECOMBINATION; TEMPERATURE EFFECT;

ARTICLE; BARLEY; CATALYSIS; CONCENTRATION (PARAMETERS); DNA LIBRARY; ENZYME ACTIVITY; ENZYME ANALYSIS; ESCHERICHIA COLI; FUNGAL STRAIN; GENE EXPRESSION; HYDROLYSIS; HYPOCREA JECORINA; INCUBATION TIME; METAGENOME; METAGENOMICS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; PROTEIN EXPRESSION; TEMPERATURE; THERMOSTABILITY; THIN LAYER CHROMATOGRAPHY;

BACTERIA; BACTERIAL PROTEINS; BIOFUELS; CELLULASE; ENZYME STABILITY; FUNGAL PROTEINS; GENE EXPRESSION; METAGENOME; MOLECULAR SEQUENCE DATA; TRICHODERMA;

ESCHERICHIA COLI; FUNGI; HORDEUM; HYPOCREA JECORINA; SUIDAE; ZEA MAYS;

EID: 84864567188     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-3873-y     Document Type: Article

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