-
2
-
-
0022558555
-
Purification, reconstitution, and characterization of the lac permease of Escherichia coli
-
Viitanen P, Newman MJ, Foster DL, Wilson TH, Kaback HR (1986) Purification, reconstitution, and characterization of the lac permease of Escherichia coli. Methods Enzymol 125:429-452. (Pubitemid 16072654)
-
(1986)
Methods in Enzymology
, vol.VOL. 125
, pp. 429-452
-
-
Viitanen, P.1
Newman, M.J.2
Foster, D.L.3
-
3
-
-
0037133698
-
Surface-exposed positions in the transmembrane helices of the lactose permease of Escherichia coli determined by intermolecular thiol cross-linking
-
DOI 10.1073/pnas.052703699
-
Guan L, Murphy FD, Kaback HR (2002) Surface-exposed positions in the transmembrane helices of the lactose permease of Escherichia coli determined by intermolecular thiol cross-linking. Proc Natl Acad Sci USA 99:3475-3480. (Pubitemid 34252133)
-
(2002)
Proceedings of the National Academy of Sciences of the United States of America
, vol.99
, Issue.6
, pp. 3475-3480
-
-
Guan, L.1
Murphy, F.D.2
Kaback, H.R.3
-
5
-
-
0028291250
-
Properties of permease dimer, a fusion protein containing two lactose permease molecules from Escherichia coli
-
DOI 10.1073/pnas.91.12.5421
-
Sahin-TóthM, Lawrence MC, Kaback HR (1994) Properties of permease dimer, a fusion protein containing two lactose permease molecules from Escherichia coli. Proc Natl Acad Sci USA 91:5421-5425. (Pubitemid 24174339)
-
(1994)
Proceedings of the National Academy of Sciences of the United States of America
, vol.91
, Issue.12
, pp. 5421-5425
-
-
Sahin-Toth, M.1
Lawrence, M.C.2
Kaback, H.R.3
-
6
-
-
0041353145
-
Structure and mechanism of the lactose permease of Escherichia coli
-
DOI 10.1126/science.1088196
-
Abramson J, et al. (2003) Structure and mechanism of the lactose permease of Escherichia coli. Science 301:610-615. (Pubitemid 36927939)
-
(2003)
Science
, vol.301
, Issue.5633
, pp. 610-615
-
-
Abramson, J.1
Smirnova, I.2
Kasho, V.3
Verner, G.4
Kaback, H.R.5
Iwata, S.6
-
7
-
-
33645320817
-
Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY
-
Mirza O, Guan L, Verner G, Iwata S, Kaback HR (2006) Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY. EMBO J 25:1177-1183.
-
(2006)
EMBO J
, vol.25
, pp. 1177-1183
-
-
Mirza, O.1
Guan, L.2
Verner, G.3
Iwata, S.4
Kaback, H.R.5
-
8
-
-
34848888055
-
Structural determination of wild-type lactose permease
-
DOI 10.1073/pnas.0707688104
-
Guan L, Mirza O, Verner G, Iwata S, Kaback HR (2007) Structural determination of wild-type lactose permease. Proc Natl Acad Sci USA 104:15294-15298. (Pubitemid 47502911)
-
(2007)
Proceedings of the National Academy of Sciences of the United States of America
, vol.104
, Issue.39
, pp. 15294-15298
-
-
Guan, L.1
Mirza, O.2
Verner, G.3
Iwata, S.4
Kaback, H.R.5
-
9
-
-
79959349280
-
Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition
-
Chaptal V, et al. (2011) Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition. Proc Natl Acad Sci USA 108:9361-9366.
-
(2011)
Proc Natl Acad Sci USA
, vol.108
, pp. 9361-9366
-
-
Chaptal, V.1
-
10
-
-
33846277908
-
Site-directed alkylation and the alternating access model for LacY
-
DOI 10.1073/pnas.0609968104
-
Kaback HR, et al. (2007) Site-directed alkylation and the alternating access model for LacY. Proc Natl Acad Sci USA 104:491-494. (Pubitemid 46123053)
-
(2007)
Proceedings of the National Academy of Sciences of the United States of America
, vol.104
, Issue.2
, pp. 491-494
-
-
Kaback, H.R.1
Dunten, R.2
Frillingos, S.3
Venkatesan, P.4
Kwaw, I.5
Zhang, W.6
Ermolova, N.7
-
11
-
-
34547880034
-
Single-molecule FRET reveals sugar-induced conformational dynamics in LacY
-
DOI 10.1073/pnas.0700969104
-
Majumdar DS, et al. (2007) Single-molecule FRET reveals sugar-induced conformational dynamics in LacY. Proc Natl Acad Sci USA 104:12640-12645. (Pubitemid 47255207)
-
(2007)
Proceedings of the National Academy of Sciences of the United States of America
, vol.104
, Issue.31
, pp. 12640-12645
-
-
Majumdar, D.S.1
Smirnova, I.2
Kasho, V.3
Nir, E.4
Kong, X.5
Weiss, S.6
Kaback, H.R.7
-
12
-
-
36749074450
-
Sugar binding induces an outward facing conformation of LacY
-
DOI 10.1073/pnas.0708258104
-
Smirnova I, et al. (2007) Sugar binding induces an outward facing conformation of LacY. Proc Natl Acad Sci USA 104:16504-16509. (Pubitemid 350211048)
-
(2007)
Proceedings of the National Academy of Sciences of the United States of America
, vol.104
, Issue.42
, pp. 16504-16509
-
-
Smirnova, I.1
Kasho, V.2
Choe, J.-Y.3
Altenbach, C.4
Hubbell, W.L.5
Kaback, H.R.6
-
13
-
-
35548929298
-
Site-directed Alkylation of LacY: Effect of the Proton Electrochemical Gradient
-
DOI 10.1016/j.jmb.2007.09.006, PII S0022283607011679
-
Nie Y, Ermolova N, Kaback HR (2007) Site-directed alkylation of LacY: Effect of the proton electrochemical gradient. J Mol Biol 374:356-364. (Pubitemid 350007643)
-
(2007)
Journal of Molecular Biology
, vol.374
, Issue.2
, pp. 356-364
-
-
Nie, Y.1
Ermolova, N.2
Kaback, H.R.3
-
14
-
-
44149122380
-
The Cys154 - >Gly mutation in LacY causes constitutive opening of the hydrophilic periplasmic pathway
-
Nie Y, Sabetfard FE, Kaback HR (2008) The Cys154 - >Gly mutation in LacY causes constitutive opening of the hydrophilic periplasmic pathway. J Mol Biol 379:695-703.
-
(2008)
J Mol Biol
, vol.379
, pp. 695-703
-
-
Nie, Y.1
Sabetfard, F.E.2
Kaback, H.R.3
-
15
-
-
41649112580
-
Opening and closing of the periplasmic gate in lactose permease
-
DOI 10.1073/pnas.0800825105
-
Zhou Y, Guan L, Freites JA, Kaback HR (2008) Opening and closing of the periplasmic gate in lactose permease. Proc Natl Acad Sci USA 105:3774-3778. (Pubitemid 351723474)
-
(2008)
Proceedings of the National Academy of Sciences of the United States of America
, vol.105
, Issue.10
, pp. 3774-3778
-
-
Zhou, Y.1
Guan, L.2
Freites, J.A.3
Kaback, H.R.4
-
16
-
-
77953101915
-
Sugar binding induces the same global conformational change in purified LacY as in the native bacterial membrane
-
Nie Y, Kaback HR (2010) Sugar binding induces the same global conformational change in purified LacY as in the native bacterial membrane. Proc Natl Acad Sci USA 107:9903-9908.
-
(2010)
Proc Natl Acad Sci USA
, vol.107
, pp. 9903-9908
-
-
Nie, Y.1
Kaback, H.R.2
-
17
-
-
79952369030
-
Site-directed alkylation studies with LacY provide evidence for the alternating access model of transport
-
Jiang X, Nie Y, Kaback HR (2011) Site-directed alkylation studies with LacY provide evidence for the alternating access model of transport. Biochemistry 50:1634-1640.
-
(2011)
Biochemistry
, vol.50
, pp. 1634-1640
-
-
Jiang, X.1
Nie, Y.2
Kaback, H.R.3
-
18
-
-
79955662093
-
The alternating access transport mechanism in LacY
-
Kaback HR, Smirnova I, Kasho V, Nie Y, Zhou Y (2011) The alternating access transport mechanism in LacY. J Membr Biol 239:85-93.
-
(2011)
J Membr Biol
, vol.239
, pp. 85-93
-
-
Kaback, H.R.1
Smirnova, I.2
Kasho, V.3
Nie, Y.4
Zhou, Y.5
-
19
-
-
80053056248
-
Opening the periplasmic cavity in lactose permease is the limiting step for sugar binding
-
Smirnova I, Kasho V, Sugihara J, Kaback HR (2011) Opening the periplasmic cavity in lactose permease is the limiting step for sugar binding. Proc Natl Acad Sci USA 108:15147-15151.
-
(2011)
Proc Natl Acad Sci USA
, vol.108
, pp. 15147-15151
-
-
Smirnova, I.1
Kasho, V.2
Sugihara, J.3
Kaback, H.R.4
-
20
-
-
53849119555
-
Ins and outs of major facilitator superfamily antiporters
-
Law CJ, Maloney PC, Wang DN (2008) Ins and outs of major facilitator superfamily antiporters. Annu Rev Microbiol 62:289-305.
-
(2008)
Annu Rev Microbiol
, vol.62
, pp. 289-305
-
-
Law, C.J.1
Maloney, P.C.2
Wang, D.N.3
-
21
-
-
84855351908
-
Insights into transport mechanism from LeuT engineered to transport tryptopan
-
Piscitelli CL, Gouaux B (2011) Insights into transport mechanism from LeuT engineered to transport tryptopan. EMBO J 31:228-235.
-
(2011)
EMBO J
, vol.31
, pp. 228-235
-
-
Piscitelli, C.L.1
Gouaux, B.2
-
22
-
-
84856225222
-
X-ray structures of LeuT in substrate-free outwardopen and apo inward-open states
-
Krishnamurthy H, Gouaux E (2012) X-ray structures of LeuT in substrate-free outwardopen and apo inward-open states. Nature 481:469-474.
-
(2012)
Nature
, vol.481
, pp. 469-474
-
-
Krishnamurthy, H.1
Gouaux, E.2
-
23
-
-
33748644877
-
Structure of a bacterial multidrug ABC transporter
-
DOI 10.1038/nature05155, PII NATURE05155
-
Dawson RJ, Locher KP (2006) Structure of a bacterial multidrug ABC transporter. Nature 443:180-185. (Pubitemid 44387602)
-
(2006)
Nature
, vol.443
, Issue.7108
, pp. 180-185
-
-
Dawson, R.J.P.1
Locher, K.P.2
-
24
-
-
60549097035
-
Alternating access in maltose transporter mediated by rigid-body rotations
-
Khare D, Oldham ML, Orelle C, Davidson AL, Chen J (2009) Alternating access in maltose transporter mediated by rigid-body rotations. Mol Cell 33:528-536.
-
(2009)
Mol Cell
, vol.33
, pp. 528-536
-
-
Khare, D.1
Oldham, M.L.2
Orelle, C.3
Davidson, A.L.4
Chen, J.5
-
25
-
-
76049089794
-
Probing of the rates of alternating access in LacY with Trp fluorescence
-
Smirnova I, Kasho V, Sugihara J, Kaback HR (2009) Probing of the rates of alternating access in LacY with Trp fluorescence. Proc Natl Acad Sci USA 106:21561-21566.
-
(2009)
Proc Natl Acad Sci USA
, vol.106
, pp. 21561-21566
-
-
Smirnova, I.1
Kasho, V.2
Sugihara, J.3
Kaback, H.R.4
-
26
-
-
84863369867
-
Evidence for an intermediate conformational state of LacY
-
Jiang X, et al. (2012) Evidence for an intermediate conformational state of LacY. Proc Natl Acad Sci USA 109:E698-E704.
-
(2012)
Proc Natl Acad Sci USA
, vol.109
-
-
Jiang, X.1
-
27
-
-
0030885252
-
The lipid bilayer determines helical tilt angle and function in lactose permease of Escherichia coli
-
le Coutre J, Narasimhan LR, Patel CK, Kaback HR (1997) The lipid bilayer determines helical tilt angle and function in lactose permease of Escherichia coli. Proc Natl Acad Sci USA 94:10167-10171.
-
(1997)
Proc Natl Acad Sci USA
, vol.94
, pp. 10167-10171
-
-
Le Coutre, J.1
Narasimhan, L.R.2
Patel, C.K.3
Kaback, H.R.4
-
28
-
-
0032568559
-
Fourier transform infrared spectroscopy reveals a rigid alpha-helical assembly for the tetrameric Streptomyces lividans K+ channel
-
le Coutre J, Kaback HR, Patel CK, Heginbotham L, Miller C (1998) Fourier transform infrared spectroscopy reveals a rigid alpha-helical assembly for the tetrameric Streptomyces lividans K+ channel. Proc Natl Acad Sci USA 95:6114-6117.
-
(1998)
Proc Natl Acad Sci USA
, vol.95
, pp. 6114-6117
-
-
Le Coutre, J.1
Kaback, H.R.2
Patel, C.K.3
Heginbotham, L.4
Miller, C.5
-
29
-
-
0032480817
-
Fourier transform infrared analysis of purified lactose permease: A monodisperse lactose permease preparation is stably folded, alpha-helical, and highly accessible to deuterium exchange
-
DOI 10.1021/bi981142x
-
Patzlaff JS, Moeller JA, Barry BA, Brooker RJ (1998) Fourier transform infrared analysis of purified lactose permease: a monodisperse lactose permease preparation is stably folded, alpha-helical, and highly accessible to deuterium exchange. Biochemistry 37:15363-15375. (Pubitemid 28515997)
-
(1998)
Biochemistry
, vol.37
, Issue.44
, pp. 15363-15375
-
-
Patzlaff, J.S.1
Moeller, J.A.2
Barry, B.A.3
Brooker, R.J.4
-
30
-
-
33845917073
-
Energetics of ligand-induced conformational flexibility in the lactose permease of Escherichia coli
-
DOI 10.1074/jbc.M607232200
-
Nie Y, Smirnova I, Kasho V, Kaback HR (2006) Energetics of ligand-induced conformational flexibility in the lactose permease of Escherichia coli. J Biol Chem 281:35779-35784. (Pubitemid 46041309)
-
(2006)
Journal of Biological Chemistry
, vol.281
, Issue.47
, pp. 35779-35784
-
-
Nie, Y.1
Smirnova, I.2
Kasho, V.3
Kaback, H.R.4
-
31
-
-
65249190326
-
Structural characterization of the osmosensor ProP
-
Sayeed WM, Baenziger JE (2009) Structural characterization of the osmosensor ProP. Biochim Biophys Acta 1788:1108-1115.
-
(2009)
Biochim Biophys Acta
, vol.1788
, pp. 1108-1115
-
-
Sayeed, W.M.1
Baenziger, J.E.2
-
32
-
-
0031717187
-
Cys-scanning mutagenesis: A novel approach to structure-function relationships in polytopic membrane proteins
-
Frillingos S, Sahin-Tóth M, Wu J, Kaback HR (1998) Cys-scanning mutagenesis: a novel approach to structure function relationships in polytopic membrane proteins. FASEB J 12:1281-1299. (Pubitemid 28475570)
-
(1998)
FASEB Journal
, vol.12
, Issue.13
, pp. 1281-1299
-
-
Frillingos, S.1
Sahin-Toth, M.2
Wu, J.3
Kaback, H.R.4
-
34
-
-
80755122853
-
Lactose permease and the alternating access mechanism
-
Smirnova I, Kasho V, Kaback HR (2011) Lactose permease and the alternating access mechanism. Biochemistry 50:9684-9693.
-
(2011)
Biochemistry
, vol.50
, pp. 9684-9693
-
-
Smirnova, I.1
Kasho, V.2
Kaback, H.R.3
-
35
-
-
70349140554
-
Residues in the H+ translocation site define the pKa for sugar binding to LacY
-
Smirnova IN, Kasho VN, Sugihara J, Choe JY, Kaback HR (2009) Residues in the H+ translocation site define the pKa for sugar binding to LacY. Biochemistry 48:8852-8860.
-
(2009)
Biochemistry
, vol.48
, pp. 8852-8860
-
-
Smirnova, I.N.1
Kasho, V.N.2
Sugihara, J.3
Choe, J.Y.4
Kaback, H.R.5
-
36
-
-
0030685033
-
Cysteine-scanning mutagenesis of helix IV and the adjoining loops in the lactose permease of Escherichia coli: Glu126 and Arg144 are essential
-
DOI 10.1021/bi972314d
-
Frillingos S, Gonzalez A, Kaback HR (1997) Cysteine-scanning mutagenesis of helix IV and the adjoining loops in the lactose permease of Escherichia coli: Glu126 and Arg144 are essential. off. Biochemistry 36:14284-14290. (Pubitemid 27509920)
-
(1997)
Biochemistry
, vol.36
, Issue.47
, pp. 14284-14290
-
-
Frillingos, S.1
Gonzalez, A.2
Kaback, H.R.3
-
37
-
-
0038257492
-
Characterization of Glu126 and Arg144, two residues that are indispensable for substrate binding in the lactose permease of Escherichia coli
-
Sahin-Tóth M, et al. (1999) Characterization of Glu126 and Arg144, two residues that are indispensable for substrate binding in the lactose permease of Escherichia coli. Biochemistry 38:813-819.
-
(1999)
Biochemistry
, vol.38
, pp. 813-819
-
-
Sahin-Tóth, M.1
-
38
-
-
33745045462
-
+ Symport by the Sucrose Permease of Escherichia coli Relative to Lactose Permease
-
DOI 10.1016/j.jmb.2006.02.050, PII S002228360600249X
-
Vadyvaloo V, Smirnova IN, Kasho VN, Kaback HR (2006) Conservation of residues involved in sugar/H(+) symport by the sucrose permease of Escherichia coli relative to lactose permease. J Mol Biol 358:1051-1059. (Pubitemid 44382450)
-
(2006)
Journal of Molecular Biology
, vol.358
, Issue.4
, pp. 1051-1059
-
-
Vadyvaloo, V.1
Smirnova, I.N.2
Kasho, V.N.3
Ronald, K.H.4
-
40
-
-
0028234751
-
Functional roles of Glu-269 and Glu-325 within the lactose permease of Escherichia coli
-
Franco PJ, Brooker RJ (1994) Functional roles of Glu-269 and Glu-325 within the lactose permease of Escherichia coli. J Biol Chem 269:7379-7386. (Pubitemid 24217935)
-
(1994)
Journal of Biological Chemistry
, vol.269
, Issue.10
, pp. 7379-7386
-
-
Franco, P.J.1
Brooker, R.J.2
-
41
-
-
0023047638
-
Lac permease of Escherichia coli: Histidine-322 and glutamic acid-325 may be components of a charge-relay system
-
Carrasco N, Antes LM, Poonian MS, Kaback HR (1986) lac permease of Escherichia coli: Histidine-322 and glutamic acid-325 may be components of a charge-relay system. Biochemistry 25:4486-4488.
-
(1986)
Biochemistry
, vol.25
, pp. 4486-4488
-
-
Carrasco, N.1
Antes, L.M.2
Poonian, M.S.3
Kaback, H.R.4
-
42
-
-
0024562578
-
Characterization of site-directed mutants in the lac permease of Escherichia coli. 2. Glutamate-325 replacements
-
DOI 10.1021/bi00432a028
-
Carrasco N, et al. (1989) Characterization of site-directed mutants in the lac permease of Escherichia coli. 2. Glutamate-325 replacements. Biochemistry 28:2533-2539. (Pubitemid 19094371)
-
(1989)
Biochemistry
, vol.28
, Issue.6
, pp. 2533-2539
-
-
Carrasco, N.1
Puttner, I.B.2
Antes, L.M.3
Lee, J.A.4
Larigan, J.D.5
Lolkema, J.S.6
Roepe, P.D.7
Kaback, H.R.8
-
44
-
-
79952738070
-
The alternating-access mechanism of MFS transporters arises from inverted-topology repeats
-
Radestock S, Forrest LR (2011) The alternating-access mechanism of MFS transporters arises from inverted-topology repeats. J Mol Biol 407:698-715.
-
(2011)
J Mol Biol
, vol.407
, pp. 698-715
-
-
Radestock, S.1
Forrest, L.R.2
-
45
-
-
0026757193
-
Possible salt bridges between transmembrane alpha-helices of the lactose carrier of Escherichia coli
-
Lee JI, Hwang PP, Hansen C, Wilson TH (1992) Possible salt bridges between transmembrane alpha-helices of the lactose carrier of Escherichia coli. J Biol Chem 267:20758-20764.
-
(1992)
J Biol Chem
, vol.267
, pp. 20758-20764
-
-
Lee, J.I.1
Hwang, P.P.2
Hansen, C.3
Wilson, T.H.4
-
46
-
-
0026439214
-
Functional interactions between putative intramembrane charged residues in the lactose permease of Escherichia coli
-
Sahin-Tóth M, Dunten RL, Gonzalez A, Kaback HR (1992) Functional interactions between putative intramembrane charged residues in the lactose permease of Escherichia coli. Proc Natl Acad Sci USA 89:10547-10551.
-
(1992)
Proc Natl Acad Sci USA
, vol.89
, pp. 10547-10551
-
-
Sahin-Tóth, M.1
Dunten, R.L.2
Gonzalez, A.3
Kaback, H.R.4
-
47
-
-
0027382884
-
Properties of interacting aspartic acid and lysine residues in the lactose permease of Escherichia coli
-
DOI 10.1021/bi00089a019
-
Sahin-Tóth M, Kaback HR (1993) Properties of interacting aspartic acid and lysine residues in the lactose permease of Escherichia coli. Biochemistry 32:10027-10035. (Pubitemid 23312429)
-
(1993)
Biochemistry
, vol.32
, Issue.38
, pp. 10027-10035
-
-
Sahin-Toth, M.1
Kaback, H.R.2
-
48
-
-
0023825249
-
Bioenergetic coupling to protonmotive force: Should we be considering hydronium ion coordination and not group protonation?
-
Boyer PD (1988) Bioenergetic coupling to protonmotive force: Should we be considering hydronium ion coordination and not group protonation? Trends Biochem Sci 13:5-7.
-
(1988)
Trends Biochem Sci
, vol.13
, pp. 5-7
-
-
Boyer, P.D.1
-
49
-
-
35448959682
-
Two distinct proton binding sites in the ATP synthase family
-
von Ballmoos C, Dimroth P (2007) Two distinct proton binding sites in the ATP synthase family. Biochemistry 46:11800-11809.
-
(2007)
Biochemistry
, vol.46
, pp. 11800-11809
-
-
Von Ballmoos, C.1
Dimroth, P.2
-
50
-
-
0018850485
-
Lactose carrier protein of Escherichia coli. Structure and expression of plasmids carrying the Y gene of the lac operon
-
Teather RM, et al. (1980) Lactose carrier protein of Escherichia coli. Structure and expression of plasmids carrying the Y gene of the lac operon. Eur J Biochem 108:223-231.
-
(1980)
Eur J Biochem
, vol.108
, pp. 223-231
-
-
Teather, R.M.1
-
51
-
-
77957010716
-
Bacterial membranes
-
eds Kaplan NP, Jakoby WB, Colowick NP (Elsevier, New York)
-
Kaback HR (1971) Bacterial membranes. Methods in Enzymology, eds Kaplan NP, Jakoby WB, Colowick NP (Elsevier, New York), Vol XXII, pp 99-120.
-
(1971)
Methods in Enzymology
, vol.22
, pp. 99-120
-
-
Kaback, H.R.1
-
52
-
-
0016750458
-
Localization of D-lactate dehydrogenase in native and reconstituted Escherichia coli membrane vesicles
-
Short SA, Kaback HR, Kohn LD (1975) Localization of D-lactate dehydrogenase in native and reconstituted Escherichia coli membrane vesicles. J Biol Chem 250:4291-4296.
-
(1975)
J Biol Chem
, vol.250
, pp. 4291-4296
-
-
Short, S.A.1
Kaback, H.R.2
Kohn, L.D.3
|