Residues in the H+ translocation site define the pKa for sugar binding to LacY

Biochemistry

Volumn 48, Issue 37, 2009, Pages 8852-8860

  Smirnova, Irina ^a   Kasho, Vladimir ^a   Sugihara, Junichi ^a   Choe, Jun Yong ^c   Kaback, H Ronald ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c ROSALIND FRANKLIN UNIVERSITY OF MEDICINE AND SCIENCE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PH; AMINO ACID RESIDUES; BINDING AFFINITIES; DISSOCIATION CONSTANT; HIGH AFFINITY; LACTOSE PERMEASE; NEUTRAL PH; PH DEPENDENCE; PHYSIOLOGICAL CONDITION; PROTONATED; STOPPED-FLOW FLUOROMETRY; STRUCTURAL EVIDENCE; SUBSTRATE BINDS; SUGAR BINDING; WILD TYPES;

AMINES; AMINO ACIDS; BINDING ENERGY; BINDING SITES; DISSOCIATION; ESCHERICHIA COLI; FLUOROMETERS; ORGANIC ACIDS; RATE CONSTANTS; SUGAR (SUCROSE); SUGARS;

PH EFFECTS;

ARGININE; ASPARTIC ACID; BACTERIAL ENZYME; GLUTAMIC ACID; HISTIDINE; HYDROGEN; LACY ENZYME; LYSINE; SUGAR; TYROSINE; UNCLASSIFIED DRUG;

ALKALINITY; ARTICLE; BINDING AFFINITY; BINDING SITE; ENZYME BINDING; ENZYME STRUCTURE; ESCHERICHIA COLI; FLUOROMETRY; GENE MUTATION; NONHUMAN; PH MEASUREMENT; PKA; PRIORITY JOURNAL; PROTON TRANSPORT; STEADY STATE; WILD TYPE;

AMINO ACID SUBSTITUTION; ESCHERICHIA COLI PROTEINS; HYDROGEN-ION CONCENTRATION; LACTOSE; MONOSACCHARIDE TRANSPORT PROTEINS; ONIUM COMPOUNDS; PROTEIN BINDING; PROTEIN TRANSPORT; PROTONS; SUBSTRATE SPECIFICITY; SYMPORTERS;

ESCHERICHIA COLI;

EID: 70349140554     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9011918     Document Type: Article

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