Sugar binding induces the same global conformational change in purified LacY as in the native bacterial membrane

Proceedings of the National Academy of Sciences of the United States of America

Volumn 107, Issue 21, 2010, Pages 9903-9908

  Nie, Yiling ^a   Kaback, H Ronald ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Lactose; Membrane proteins; Permease; Symport; Transport

Indexed keywords

BETA DEXTRO GALACTOPYRANOSYL 1 THIO BETA DEXTRO GALACTOPYRANOSIDE; DETERGENT; LACTOSE PERMEASE; PYRANOSIDE; UNCLASSIFIED DRUG;

ALKYLATION; ARTICLE; BACTERIAL MEMBRANE; BINDING SITE; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; MICELLE; NONHUMAN; PRIORITY JOURNAL;

CARBOHYDRATE METABOLISM; CARBOHYDRATES; CELL MEMBRANE; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MICELLES; MODELS, MOLECULAR; MONOSACCHARIDE TRANSPORT PROTEINS; MUTATION; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; SOLUBILITY; SYMPORTERS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 77953101915     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1004515107     Document Type: Article

References (34)

1. Viitanen P, Newman MJ, Foster DL, Wilson TH, Kaback HR (1986) Purification, reconstitution, and characterization of the lac permease of Escherichia coli. Methods Enzymol 125:429-452. (Pubitemid 16072654)
2. Menick DR, Sarkar HK, Poonian MS, Kaback HR (1985) cys154 Is important for lac permease activity in Escherichia coli. Biochem Biophys Res Commun 132:162-170. (Pubitemid 16196125)
3. Smirnova IN, Kaback HR (2003) A mutation in the lactose permease of Escherichia coli that decreases conformational flexibility and increases protein stability. Biochemistry 42:3025-3031.
4. Ermolova NV, Smirnova IN, Kasho VN, Kaback HR (2005) Interhelical packing modulates conformational flexibility in the lactose permease of Escherichia coli. Biochemistry 44:7669-7677.
5. Nie Y, Smirnova I, Kasho V, Kaback HR (2006) Energetics of ligand-induced conformational flexibility in the lactose permease of Escherichia coli. J Biol Chem 281: 35779-35784. (Pubitemid 46041309)
6. Majumdar DS, et al. (2007) Single-molecule FRET reveals sugar-induced conformational dynamics in LacY. Proc Natl Acad Sci USA 104:12640-12645. (Pubitemid 47255207)
7. Smirnova I, et al. (2007) Sugar binding induces an outward facing conformation of LacY. Proc Natl Acad Sci USA 104:16504-16509. (Pubitemid 350211048)
8. Abramson J, et al. (2003) Structure and mechanism of the lactose permease of Escherichia coli. Science 301:610-615. (Pubitemid 36927939)
9. Mirza O, Guan L, Verner G, Iwata S, Kaback HR (2006) Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY. EMBO J 25:1177-1183.
10. Guan L, et al. (2006) Manipulating phospholipids for crystallization of a membrane transport protein. Proc Natl Acad Sci USA 103:1723-1726. (Pubitemid 43228760)
11. Guan L, Mirza O, Verner G, Iwata S, Kaback HR (2007) Structural determination of wild-type lactose permease. Proc Natl Acad Sci USA 104:15294-15298. (Pubitemid 47502911)
12. + translocation site define the pKa for sugar binding to LacY. Biochemistry 48 (37): 8852-8860.
13. + channel. Proc Natl Acad Sci USA 95:6114-6117.
14. Patzlaff JS, Moeller JA, Barry BA, Brooker RJ (1998) Fourier transform infrared analysis of purified lactose permease: A monodisperse lactose permease preparation is stably folded, alpha-helical, and highly accessible to deuterium exchange. Biochemistry 37: 15363-15375.
15. Sayeed WM, Baenziger JE (2009) Structural characterization of the osmosensor ProP. Biochim Biophys Acta 1788:1108-1115.
16. Kaback HR, Sahin-Tóth M, Weinglass AB (2001) The kamikaze approach to membrane transport. Nat Rev Mol Cell Biol 2:610-620.
17. Kaback HR (2005) Structure and mechanism of the lactose permease. C R Biol 328: 557-567.
18. Guan L, Kaback HR (2006) Lessons from lactose permease. Annu Rev Biophys Biomol Struct 35:67-91.
19. Kaback HR, et al. (2007) Site-directed alkylation and the alternating access model for LacY. Proc Natl Acad Sci USA 104:491-494.
20. Nie Y, Ermolova N, Kaback HR (2007) Site-directed alkylation of LacY: Effect of the proton electrochemical gradient. J Mol Biol 374:356-364. (Pubitemid 350007643)
21. Nie Y, Sabetfard FE, Kaback HR (2008) The Cys154→Gly mutation in LacY causes constitutive opening of the hydrophilic periplasmic pathway. J Mol Biol 379:695-703.
22. Zhou Y, Guan L, Freites JA, Kaback HR (2008) Opening and closing of the periplasmic gate in lactose permease. Proc Natl Acad Sci USA 105:3774-3778. (Pubitemid 351723474)
23. Smirnova I, Kasho V, Sugihara J, Kaback HR (2009) Probing of the rates of alternating access in LacY with Trp fluorescence. Proc Natl Acad Sci USA 106:21561-21566.
24. Zhou Y, Nie Y, Kaback HR (2009) Residues gating the periplasmic pathway of LacY. J Mol Biol 394:219-225.
25. Liu Z, Madej MG, Kaback HR (2010) Helix dynamics in LacY: Helices II and IV. J Mol Biol 396:617-626.
26. Nie Y, Zhou Y, Kaback HR (2009) Clogging the periplasmic pathway in LacY. Biochemistry 48:738-743.
27. Venkatesan P, Kwaw I, Hu Y, Kaback HR (2000) Site-directed sulfhydryl labeling of the lactose permease of Escherichia coli: Helix VII. Biochemistry 39:10641-10648.
28. Venkatesan P, Kaback HR (1998) The substrate-binding site in the lactose permease of Escherichia coli. Proc Natl Acad Sci USA 95:9802-9807. (Pubitemid 28415250)
29. Venkatesan P, Liu Z, Hu Y, Kaback HR (2000) Site-directed sulfhydryl labeling of the lactose permease of Escherichia coli: N-ethylmaleimide-sensitive face of helix II. Biochemistry 39:10649-10655.
30. le Coutre J, Narasimhan LR, Patel CK, Kaback HR (1997) The lipid bilayer determines helical tilt angle and function in lactose permease of Escherichia coli. Proc Natl Acad Sci USA 94:10167-10171.
31. Bogdanov M, Heacock PN, Dowhan W (2002) A polytopic membrane protein displays a reversible topology dependent on membrane lipid composition. EMBO J 21:2107-2116. (Pubitemid 34516769)
32. Garcia-Celma JJ, Smirnova IN, Kaback HR, Fendler K (2009) Electrophysiological characterization of LacY. Proc Natl Acad Sci USA 106:7373-7378.
33. Kaback HR (1971) Bacterial membranes. Methods Enzymol 32:99-120.
34. Short SA, Kaback HR, Kohn LD (1975) Localization of D-lactate dehydrogenase in native and reconstituted Escherichia coli membrane vesicles. J Biol Chem 250:4291-4296.