Human butyrylcholinesterase produced in insect cells: Huprine-based affinity purification and crystal structure

FEBS Journal

Volumn 279, Issue 16, 2012, Pages 2905-2916

  Brazzolotto, Xavier ^a   Wandhammer, Marielle ^a   Ronco, Cyril ^b,d   Trovaslet, Marie ^a   Jean, Ludovic ^b   Lockridge, Oksana ^c   Renard, Pierre Yves ^b   Nachon, Florian ^a  

^a INSTITUT DE RECHERCHE BIOMÉDICALE DES ARMÉES   (France)

^b IRCOF   (France)

^c UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

^d FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

Author keywords

affinity chromatography; butyrylcholinesterase; huprine; insect cells; X ray structure

Indexed keywords

CHOLINESTERASE; RECOMBINANT ENZYME;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; CHO CELL; CONTROLLED STUDY; CRYSTAL STRUCTURE; DROSOPHILA; ENZYME ACTIVE SITE; ENZYME ASSAY; ENZYME GLYCOSYLATION; ENZYME PURIFICATION; EXPRESSION VECTOR; GENETIC TRANSFECTION; HUPRINE BASED AFFINITY PURIFICATION; INSECT CELL; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; X RAY DIFFRACTION;

AMINO ACID SEQUENCE; AMINOQUINOLINES; ANIMALS; BUTYRYLCHOLINESTERASE; CATALYTIC DOMAIN; CHO CELLS; CHROMATOGRAPHY, AFFINITY; CRICETINAE; CRICETULUS; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; DROSOPHILA; GLYCOSYLATION; HETEROCYCLIC COMPOUNDS WITH 4 OR MORE RINGS; HUMANS; KINETICS; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS;

CRICETULUS GRISEUS; HEXAPODA; MAMMALIA;

EID: 84864417831     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2012.08672.x     Document Type: Article

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