Correlated Light Microscopy and Electron Microscopy

Methods in Cell Biology

Volumn 111, Issue , 2012, Pages 157-173

  Sjollema, Klaas A ^a   Schnell, Ulrike ^a   Kuipers, Jeroen ^a   Kalicharan, Ruby ^a   Giepmans, Ben N G ^a  

^a UNIVERSITY MEDICAL CENTER GRONINGEN   (Netherlands)

Author keywords

Correlated microscopy; Drawing tube; Electron microscopy; High resolution; Light microscopy; Mosaic microscopy; Orientation; Probes

Indexed keywords

EID: 84864387195     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-416026-2.00009-1     Document Type: Chapter

References (58)

1. Agronskaia A.V., Valentijn J.A., van Driel L.F., Schneijdenberg C.T., Humbel B.M., van Bergen en Henegouwen P.M., et al. Integrated fluorescence and transmission electron microscopy. Journal of Structural Biology 2008, 164:183-189.
2. Anderson J.R., Jones B.W., Yang J.H., Shaw M.V., Watt C.B., Koshevoy P., et al. A computational framework for ultrastructural mapping of neural circuitry. PLoS Biology 2009, 7:e1000074.
3. Auinger S., Small J.V. Correlated light and electron microscopy of the cytoskeleton. Methods in Cell Biology 2008, 88:257-272.
4. Betzig E., Patterson G.H., Sougrat R., Lindwasser O.W., Olenych S., Bonifacino J.S., et al. Imaging intracellular fluorescent proteins at near-molecular resolution. Science 2006, 313:1642-1645.
5. Bos E., SantAnna C., Gnaegi H., Pinto R.F., Ravelli R.B., Koster A.J., et al. A new approach to improve the quality of ultrathin cryo-sections; its use for immunogold EM and correlative electron cryo-tomography. Journal of Structural Biology 2011, 175:62-72.
6. Briegel A., Chen S., Koster A.J., Plitzko J.M., Schwartz C.L., Jensen G.J. Correlated light and electron cryo-microscopy. Methods Enzymology 2010, 481:317-341.
7. Briggman K.L., Helmstaedter M., Denk W. Wiring specificity in the direction-selectivity circuit of the retina. Nature 2011, 471:183-188.
8. Cardona A., Saalfeld S., Preibisch S., Schmid B., Cheng A., Pulokas J., et al. An integrated micro- and macroarchitectural analysis of the Drosophila brain by computer-assisted serial section electron microscopy. PLoS Biology 2010, 8:e1000502.
9. Carlson D.B., Evans J.E. Low-cost cryo-light microscopy stage fabrication for correlated light/electron microscopy. Journal of Visualized Experiments 2011, (52). pii: 2909. 10.3791/2909.
10. Chow S.K., Hakozaki H., Price D.L., MacLean N.A., Deerinck T.J., Bouwer J.C., et al. Automated microscopy system for mosaic acquisition and processing. Journal of Microscopy 2006, 222:76-84.
11. Cortese K., Diaspro A., Tacchetti C. Advanced correlative light/electron microscopy: current methods and new developments using Tokuyasu cryosections. The Journal of Histochemistry and Cytochemistry 2009, 57:1103-1112.
12. Dantuma N.P., Pijnenburg M.A., Diederen J.H., Van der Horst D.J. Electron microscopic visualization of receptor-mediated endocytosis of Dil-labeled lipoproteins by diaminobenzidine photoconversion. The Journal of Histochemistry and Cytochemistry 1998, 46:1085-1089.
13. Darcy K.J., Staras K., Collinson L.M., Goda Y. An ultrastructural readout of fluorescence recovery after photobleaching using correlative light and electron microscopy. Nature Protocols 2006, 1:988-994.
14. de Jonge N., Ross F.M. Electron microscopy of specimens in liquid. Nature Nanotechnology 2011, 6:695-704.
15. Deerinck T.J., Martone M.E., Lev-Ram V., Green D.P., Tsien R.Y., Spector D.L., et al. Fluorescence photooxidation with eosin: a method for high resolution immunolocalization and in situ hybridization detection for light and electron microscopy. The Journal of Cell Biology 1994, 126:901-910.
16. Dukes M.J., Peckys D.B., de Jonge N. Correlative fluorescence microscopy and scanning transmission electron microscopy of quantum-dot-labeled proteins in whole cells in liquid. ACS Nano 2010, 4:4110-4116.
17. Giepmans B.N., Deerinck T.J., Smarr B.L., Jones Y.Z., Ellisman M.H. Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots. Nature Methods 2005, 2:743-749.
18. Giepmans B.N., Adams S.R., Ellisman M.H., Tsien R.Y. The fluorescent toolbox for assessing protein location and function. Science 2006, 312:217-224.
19. Giepmans B.N. Bridging fluorescence microscopy and electron microscopy. Histochemistry and Cell Biology 2008, 130:211-217.
20. Hanson H.H., Reilly J.E., Lee R., Janssen W.G., Phillips G.R. Streamlined embedding of cell monolayers on gridded glass-bottom imaging dishes for correlative light and electron microscopy. Microscopy and Microanalysis 2010, 16:747-754.
21. Jahn K.A., Barton D.A., Su Y., Riches J., Kable E.P., Soon L.L., et al. Correlative fluorescence and transmission electron microscopy: an elegant tool to study the actin cytoskeleton of whole-mount (breast) cancer cells. Journal of Microscopy 2009, 235:282-292.
22. Jimenez N., Van Donselaar E.G., De Winter D.A., Vocking K., Verkleij A.J., Post J.A. Gridded Aclar: preparation methods and use for correlative light and electron microscopy of cell monolayers, by TEM and FIB-SEM. Journal of Microscopy 2010, 237:208-220.
23. Kaynig V., Fischer B., Muller E., Buhmann J.M. Fully automatic stitching and distortion correction of transmission electron microscope images. Journal of Structural Biology 2010, 171:163-173.
24. Kolotuev I., Schwab Y., Labouesse M. A precise and rapid mapping protocol for correlative light and electron microscopy of small invertebrate organisms. Biology of the Cell 2009, 102:121-132.
25. Kukulski W., Schorb M., Welsch S., Picco A., Kaksonen M., Briggs J.A. Correlated fluorescence and 3D electron microscopy with high sensitivity and spatial precision. The Journal of Cell Biology 2011, 192:111-119.
26. Leung B.O., Chou K.C. Review of super-resolution fluorescence microscopy for biology. Applied Spectroscopy 2011, 65:967-980.
27. Lucic V., Kossel A.H., Yang T., Bonhoeffer T., Baumeister W., Sartori A. Multiscale imaging of neurons grown in culture: from light microscopy to cryo-electron tomography. Journal of Structural Biology 2007, 160:146-156.
28. Maranto A.R. Neuronal mapping: a photooxidation reaction makes Lucifer yellow useful for electron microscopy. Science 1982, 217:953-955.
29. McDonald K.L. A review of high-pressure freezing preparation techniques for correlative light and electron microscopy of the same cells and tissues. Journal of Microscopy 2009, 235:273-281.
30. Meisslitzer-Ruppitsch C., Rohrl C., Neumuller J., Pavelka M., Ellinger A. Photooxidation technology for correlated light and electron microscopy. Journal of Microscopy 2009, 235:322-335.
31. Merdes A., De Mey J. Kinetochore microtubules, analyzed by correlated light and immunoelectron microscopy. Methods in Molecular Biology 2011, 777:209-221.
32. Micheva K.D., Smith S.J. Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits. Neuron 2007, 55:25-36.
33. Nishiyama H., Suga M., Ogura T., Maruyama Y., Koizumi M., Mio K., et al. Atmospheric scanning electron microscope observes cells and tissues in open medium through silicon nitride film. Journal of Structural Biology 2010, 169:438-449.
34. Nisman R., Dellaire G., Ren Y., Li R., Bazett-Jones D.P. Application of quantum dots as probes for correlative fluorescence, conventional, and energy-filtered transmission electron microscopy. The Journal of Histochemistry and Cytochemistry 2004, 52:13-18.
35. Nixon S.J., Webb R.I., Floetenmeyer M., Schieber N., Lo H.P., Parton R.G. A single method for cryofixation and correlative light, electron microscopy and tomography of zebrafish embryos. Traffic 2009, 10:131-136.
36. Patterson G., Davidson M., Manley S., Lippincott-Schwartz J. Superresolution imaging using single-molecule localization. Annual Review of Physical Chemistry 2010, 61:345-367.
37. Perkins G.A., Sun M.G., Frey T.G. Chapter 2 correlated light and electron microscopy/electron tomography of mitochondria in situ. Methods Enzymology 2009, 456:29-52.
38. Robinson J.M., Takizawa T., Pombo A., Cook P.R. Correlative fluorescence and electron microscopy on ultrathin cryosections: bridging the resolution gap. The Journal of Histochemistry and Cytochemistry 2001, 49:803-808.
39. Robinson J.M., Takizawa T. Correlative fluorescence and electron microscopy in tissues: immunocytochemistry. Journal of Microscopy 2009, 235(Sep (3)):259-272.
40. Roth J., Bendayan M., Orci L. FITC-protein A-gold complex for light and electron microscopic immunocytochemistry. The Journal of Histochemistry and Cytochemistry 1980, 28:55-57.
41. Sartori A., Gatz R., Beck F., Rigort A., Baumeister W., Plitzko J.M. Correlative microscopy: bridging the gap between fluorescence light microscopy and cryo-electron tomography. Journal of Structural Biology 2007, 160:135-145.
42. Schermelleh L., Heintzmann R., Leonhardt H. A guide to super-resolution fluorescence microscopy. The Journal of Cell Biology 2010, 190:165-175.
43. Schnell U., Dijk F., Sjollema K.A., Giepmans B.N. Immunolabeling artifacts and the need for live-cell imaging. Nature Methods 2012, 9:152-158.
44. Shu X., Lev-Ram V., Deerinck T.J., Qi Y., Ramko E.B., Davidson M.W., et al. A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms. PLoS Biology 2011, 9:e1001041.
45. Sims P.A., Hardin J.D. Fluorescence-integrated transmission electron microscopy images: integrating fluorescence microscopy with transmission electron microscopy. Methods in Molecular Biology 2007, 369:291-308.
46. Sims P., Albrecht R., Pawley J.B., Centonze V., Deerink T., Hardin J. When light microscope resolution is not enough: correlational light microscope and electron microscope. Handbook of biological confocal microscopy 2006, 846-860. Springer, New York. J. Pawley (Ed.).
47. Sosinsky G.E., Giepmans B.N., Deerinck T.J., Gaietta G.M., Ellisman M.H. Markers for correlated light and electron microscopy. Methods in Cell Biology 2007, 79:575-591.
48. Sun M.G., Williams J., Munoz-Pinedo C., Perkins G.A., Brown J.M., Ellisman M.H., et al. Correlated three-dimensional light and electron microscopy reveals transformation of mitochondria during apoptosis. Nature Cell Biology 2007, 9:1057-1065.
49. Takizawa T., Suzuki K., Robinson J.M. Correlative microscopy using FluoroNanogold on ultrathin cryosections. Proof of principle. The Journal of Histochemistry and Cytochemistry 1998, 46:1097-1102.
50. Tokuyasu K.T. A technique for ultracryotomy of cell suspensions and tissues. The Journal of Cell Biology 1973, 57:551-565.
51. van Driel L.F., Valentijn J.A., Valentijn K.M., Koning R.I., Koster A.J. Tools for correlative cryo-fluorescence microscopy and cryo-electron tomography applied to whole mitochondria in human endothelial cells. European Journal of Cell Biology 2009, 88:669-684.
52. van Rijnsoever C., Oorschot V., Klumperman J. Correlative light-electron microscopy (CLEM) combining live-cell imaging and immunolabeling of ultrathin cryosections. Nature Methods 2008, 5:973-980.
53. van Weering J.R., Wijntjes R., de Wit H., Wortel J., Cornelisse L.N., Veldkamp W.J., et al. Automated analysis of secretory vesicle distribution at the ultrastructural level. Journal of Neuroscience Methods 2008, 173:83-90.
54. Verkade P. Moving EM: the Rapid Transfer System as a new tool for correlative light and electron microscopy and high throughput for high-pressure freezing. Journal of Microscopy 2008, 230:317-328.
55. Vicidomini G., Gagliani M.C., Canfora M., Cortese K., Frosi F., Santangelo C., et al. High data output and automated 3D correlative light-electron microscopy method. Traffic 2008, 9:1828-1838.
56. Vicidomini G., Gagliani M.C., Cortese K., Krieger J., Buescher P., Bianchini P., et al. A novel approach for correlative light electron microscopy analysis. Microscopy Research and Technique 2010, 73:215-224.
57. Walter N.G., Huang C.Y., Manzo A.J., Sobhy M.A. Do-it-yourself guide: how to use the modern single-molecule toolkit. Nature Methods 2008, 5:475-489.
58. Watanabe S., Punge A., Hollopeter G., Willig K.I., Hobson R.J., Davis M.W., et al. Protein localization in electron micrographs using fluorescence nanoscopy. Nature Methods 2011, 8:80-84.