메뉴 건너뛰기




Volumn 237, Issue 2, 2010, Pages 208-220

Gridded aclar: Preparation methods and use for correlative light and electron microscopy of cell monolayers, by TEM and FIB-SEM

Author keywords

Aclar; Cell monolayers; Correlative light and electron microscopy; Endothelial cells; Focused ion beam scanning electron microscope; Immunolabelling; Transmission electron microscope

Indexed keywords

ELECTRONS; FLUORESCENCE; FOCUSED ION BEAMS; HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; MONOLAYERS; SCANNING ELECTRON MICROSCOPY; SUBSTRATES; TISSUE CULTURE;

EID: 75149125224     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2009.03329.x     Document Type: Article
Times cited : (40)

References (26)
  • 2
    • 0017239547 scopus 로고
    • Receptor-mediated control of cholesterol metabolism
    • Brown, M.S. Goldstein, J.L. (1976) Receptor-mediated control of cholesterol metabolism. Science 191, 150 154.
    • (1976) Science , vol.191 , pp. 150-154
    • Brown, M.S.1    Goldstein, J.L.2
  • 3
    • 0022549920 scopus 로고
    • A receptor-mediated pathway for cholesterol homeostasis
    • Brown, M.S. Goldstein, J.L. (1986) A receptor-mediated pathway for cholesterol homeostasis. Science 232, 34 47.
    • (1986) Science , vol.232 , pp. 34-47
    • Brown, M.S.1    Goldstein, J.L.2
  • 4
    • 61449237290 scopus 로고    scopus 로고
    • Tomography of insulating biological and geological materials using focused ion beam (FIB) sectioning and low-kV BSE imaging
    • De Winter, D.A., Schneijdenberg, C.T., Lebbink, M.N., Lich, B., Verkleij, A.J., Drury, M.R. Humbel, B.M. (2009) Tomography of insulating biological and geological materials using focused ion beam (FIB) sectioning and low-kV BSE imaging. J. Microsc. 233, 372 383.
    • (2009) J. Microsc. , vol.233 , pp. 372-383
    • De Winter, D.A.1    Schneijdenberg, C.T.2    Lebbink, M.N.3    Lich, B.4    Verkleij, A.J.5    Drury, M.R.6    Humbel, B.M.7
  • 5
    • 33646235625 scopus 로고    scopus 로고
    • Protein expression dynamics during replicative senescence of endothelial cells studied by 2-D difference in-gel electrophoresis
    • Eman, M.R., Regan-Klapisz, E., Pinkse, M.W. et al 2006) Protein expression dynamics during replicative senescence of endothelial cells studied by 2-D difference in-gel electrophoresis. Electrophoresis 27, 1669 1682.
    • (2006) Electrophoresis , vol.27 , pp. 1669-1682
    • Eman, M.R.1    Regan-Klapisz, E.2    Pinkse, M.W.3
  • 6
    • 0035936802 scopus 로고    scopus 로고
    • Atherosclerosis. the road ahead
    • Glass, C.K. Witztum, J.L. (2001) Atherosclerosis. The road ahead. Cell. 104, 503 516.
    • (2001) Cell. , vol.104 , pp. 503-516
    • Glass, C.K.1    Witztum, J.L.2
  • 7
    • 69249120490 scopus 로고    scopus 로고
    • Focused ion beam-scanning electron microscope: Exploring large volumes of atherosclerotic tissue
    • Hekking, L.H., Lebbink, M.N., De Winter, D.A. et al 2009) Focused ion beam-scanning electron microscope: exploring large volumes of atherosclerotic tissue. J. Microsc., 235, 336 347.
    • (2009) J. Microsc. , vol.235 , pp. 336-347
    • Hekking, L.H.1    Lebbink, M.N.2    De Winter, D.A.3
  • 8
    • 33645244541 scopus 로고    scopus 로고
    • Aclar discs: A versatile substrate for routine high-pressure freezing of mammalian cell monolayers
    • Jiménez, N., Humbel, B.M., van Donselaar, E., Verkleij, A.J. Burger, K.N. (2006) Aclar discs: a versatile substrate for routine high-pressure freezing of mammalian cell monolayers. J. Microsc. 221, 216 223.
    • (2006) J. Microsc. , vol.221 , pp. 216-223
    • Jiménez, N.1    Humbel, B.M.2    Van Donselaar, E.3    Verkleij, A.J.4    Burger, K.N.5
  • 9
    • 60649085322 scopus 로고    scopus 로고
    • Tannic acid-mediated osmium impregnation after freeze-substitution: A strategy to enhance membrane contrast for electron tomography
    • Jiménez, N., Vocking, K., van Donselaar, E.G., Humbel, B.M., Post, J.A. Verkleij, A.J. (2009) Tannic acid-mediated osmium impregnation after freeze-substitution: a strategy to enhance membrane contrast for electron tomography. J. Struct. Biol. 166, 103 106.
    • (2009) J. Struct. Biol. , vol.166 , pp. 103-106
    • Jiménez, N.1    Vocking, K.2    Van Donselaar, E.G.3    Humbel, B.M.4    Post, J.A.5    Verkleij, A.J.6
  • 10
    • 0023681998 scopus 로고
    • Preparation of cultured mammalian cells for transmission and scanning electron microscopy using Aclar film
    • Kingsley, R.E. Cole, N.L. (1988) Preparation of cultured mammalian cells for transmission and scanning electron microscopy using Aclar film. J. Electron. Microsc. Tech. 10, 77 85.
    • (1988) J. Electron. Microsc. Tech. , vol.10 , pp. 77-85
    • Kingsley, R.E.1    Cole, N.L.2
  • 11
    • 84907130968 scopus 로고
    • Fluoroplastic coverslips for long-term nerve tissue culture
    • Masurovsky, E.B. Bunge, R.P. (1968) Fluoroplastic coverslips for long-term nerve tissue culture. Stain. Technol. 43, 161 165.
    • (1968) Stain. Technol. , vol.43 , pp. 161-165
    • Masurovsky, E.B.1    Bunge, R.P.2
  • 13
    • 75149197238 scopus 로고
    • Aclar film reticles for precise cell localization in nerve tissue culture
    • Masurovsky, E.B., Peterson, E.R. Crain, S.M. (1971) Aclar film reticles for precise cell localization in nerve tissue culture. In vitro 6, 379.
    • (1971) In Vitro , vol.6 , pp. 379
    • Masurovsky, E.B.1    Peterson, E.R.2    Crain, S.M.3
  • 14
    • 69249144515 scopus 로고    scopus 로고
    • A review of high-pressure freezing preparation techniques for correlative light and electron microscopy of the same cells and tissues
    • McDonald, K. (2009) A review of high-pressure freezing preparation techniques for correlative light and electron microscopy of the same cells and tissues. J. Microsc., 235, 273 281.
    • (2009) J. Microsc. , vol.235 , pp. 273-281
    • McDonald, K.1
  • 15
    • 0032103355 scopus 로고    scopus 로고
    • Attachment kinetics, proliferation rates and vinculin assembly of bovine osteoblasts cultured on different pre-coated artificial substrates
    • Meyer, U., Meyer, T. Jones, D.B. (1998) Attachment kinetics, proliferation rates and vinculin assembly of bovine osteoblasts cultured on different pre-coated artificial substrates. J. Mater. Sci. Mater. Med. 9, 301 307.
    • (1998) J. Mater. Sci. Mater. Med. , vol.9 , pp. 301-307
    • Meyer, U.1    Meyer, T.2    Jones, D.B.3
  • 16
    • 41749084836 scopus 로고    scopus 로고
    • Fabrication of a versatile substrate for finding samples on the nanometer scale
    • Nowak, D.B., Vattipalli, M.K., Abramson, J.J. Sanchez, E.J. (2008) Fabrication of a versatile substrate for finding samples on the nanometer scale. J. Microsc. 230, 32 41.
    • (2008) J. Microsc. , vol.230 , pp. 32-41
    • Nowak, D.B.1    Vattipalli, M.K.2    Abramson, J.J.3    Sanchez, E.J.4
  • 17
    • 0001281220 scopus 로고
    • Blood capillaries of the heart and other organs
    • Palade, G.E. (1961) Blood capillaries of the heart and other organs. Circulation 24, 368 388.
    • (1961) Circulation , vol.24 , pp. 368-388
    • Palade, G.E.1
  • 19
    • 0034627793 scopus 로고    scopus 로고
    • Correlative light-electron microscopy reveals the tubular-saccular ultrastructure of carriers operating between Golgi apparatus and plasma membrane
    • Polishchuk, R.S., Polishchuk, E.V., Marra, P., Alberti, S., Buccione, R., Luini, A. Mironov, A.A. (2000) Correlative light-electron microscopy reveals the tubular-saccular ultrastructure of carriers operating between Golgi apparatus and plasma membrane. J. Cell. Biol. 148, 45 58.
    • (2000) J. Cell. Biol. , vol.148 , pp. 45-58
    • Polishchuk, R.S.1    Polishchuk, E.V.2    Marra, P.3    Alberti, S.4    Buccione, R.5    Luini, A.6    Mironov, A.A.7
  • 20
    • 0017252175 scopus 로고
    • Morphological changes in the neuritic growth cone and target neuron during synaptic junction development in culture
    • Rees, R.P., Bunge, M.B. Bunge, R.P. (1976) Morphological changes in the neuritic growth cone and target neuron during synaptic junction development in culture. J. Cell. Biol. 68, 240 263.
    • (1976) J. Cell. Biol. , vol.68 , pp. 240-263
    • Rees, R.P.1    Bunge, M.B.2    Bunge, R.P.3
  • 21
    • 0021023703 scopus 로고
    • Cellular aspects of transcapillary exchange
    • Simionescu, N. (1983) Cellular aspects of transcapillary exchange. Physiol. Rev. 63, 1536 1579.
    • (1983) Physiol. Rev. , vol.63 , pp. 1536-1579
    • Simionescu, N.1
  • 23
    • 28544452296 scopus 로고    scopus 로고
    • Destruction of polymer growth substrates for cell cultures in two-photon microscopy
    • Thibaud, C., Koubassov, V., De Koninck, P., Chin, S.L. De Koninck, Y. (2005) Destruction of polymer growth substrates for cell cultures in two-photon microscopy. J. Microsc 220, 120 127.
    • (2005) J. Microsc , vol.220 , pp. 120-127
    • Thibaud, C.1    Koubassov, V.2    De Koninck, P.3    Chin, S.L.4    De Koninck, Y.5
  • 24
    • 0032526762 scopus 로고    scopus 로고
    • Cytochemical detection of a senescence-associated beta-galactosidase in endothelial and smooth muscle cells from human and rabbit blood vessels
    • Van Der Loo, B., Fenton, M.J. Erusalimsky, J.D. (1998) Cytochemical detection of a senescence-associated beta-galactosidase in endothelial and smooth muscle cells from human and rabbit blood vessels. Exp. Cell. Res. 241, 309 315.
    • (1998) Exp. Cell. Res. , vol.241 , pp. 309-315
    • Van Der Loo, B.1    Fenton, M.J.2    Erusalimsky, J.D.3
  • 25
    • 0020963907 scopus 로고
    • Visualization of the binding, endocytosis, and transcytosis of low-density lipoprotein in the arterial endothelium in situ
    • Vasile, E., Simionescu, M. Simionescu, N. (1983) Visualization of the binding, endocytosis, and transcytosis of low-density lipoprotein in the arterial endothelium in situ. J. Cell. Biol. 96, 1677 1689.
    • (1983) J. Cell. Biol. , vol.96 , pp. 1677-1689
    • Vasile, E.1    Simionescu, M.2    Simionescu, N.3
  • 26
    • 42649111676 scopus 로고    scopus 로고
    • Moving EM: The rapid transfer system as a new tool for correlative light and electron microscopy and high throughput for high-pressure freezing
    • Verkade, P. (2008) Moving EM: the rapid transfer system as a new tool for correlative light and electron microscopy and high throughput for high-pressure freezing. J. Microsc. 230, 317 328.
    • (2008) J. Microsc. , vol.230 , pp. 317-328
    • Verkade, P.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.