Neuroprotective effects of L-Carnitine against oxygen-glucose deprivation in rat primary cortical neurons

Korean Journal of Pediatrics

Volumn 55, Issue 7, 2012, Pages 238-248

  Kim, Yu Jin ^a   Kim, Soo Yoon ^b   Sung, Dong Kyung ^b   Chang, Yun Sil ^a   Park, Won Soon ^a  

^a Sungkyunkwan University School of Medicine   (South Korea)

^b Samsung Medical Center   (South Korea)

Author keywords

Hypoxia ischemia; L Carnitine; Neurons; Neuroprotective effect; Oxygen glucose deprivation

Indexed keywords

CARNITINE; GLUCOSE; LACTATE DEHYDROGENASE; REACTIVE OXYGEN METABOLITE;

ANIMAL CELL; ANIMAL MODEL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; BRAIN CELL; BRAIN HYPOXIA; CELL CULTURE; CELL DEATH; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; DRUG EFFECT; EMBRYO; ENZYME ACTIVITY; ENZYME RELEASE; FEMALE; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; NEUROPROTECTION; NEUROTOXICITY; NICK END LABELING; NONHUMAN; OXYGEN GLUCOSE DEPRIVATION; RAT; REOXYGENATION;

EID: 84864234364     PISSN: 17381061     EISSN: 20927258     Source Type: Journal    
DOI: 10.3345/kjp.2012.55.7.238     Document Type: Article

References (50)

1. Johnston M V, Trescher WH, Ishida A, Nakajima W. Neurobiology of hypoxic-ischemic injury in the developing brain. Pediatr Res 2001;49:735-741.
2. Vannucci RC. Hypoxic-ischemic encephalopathy. Am J Perinatol 2000; 17:113-120.
3. Aliev G, Smith MA, Obrenovich ME, de la Torre JC, Perry G. Role of vascular hypoperfusion-induced oxidative stress and mitochondria failure in the pathogenesis of Azheimer disease. Neurotox Res 2003;5:491-504.
4. Gilland E, Puka-Sundvall M, Hillered L, Hagberg H. Mitochondrial function and energy metabolism after hypoxia-ischemia in the immature rat brain: involvement of NMDA-receptors. J Cereb Blood Flow Metab 1998;18:297-304.
5. Gustavsson M, Anderson MF, Mallard C, Hagberg H. Hypoxic preconditioning confers long-term reduction of brain injury and improvement of neurological ability in immature rats. Pediatr Res 2005;57:305-309.
6. Wagner CL, Eicher DJ, Katikaneni LD, Barbosa E, Holden KR. Te use of hypothermia: a role in the treatment of neonatal asphyxia? Pediatr Neurol 1999;21:429-443.
7. Ma D, Hossain M, Pettet GK, Luo Y, Lim T, Akimov S, et al. Xenon preconditioning reduces brain damage from neonatal asphyxia in rats. J Cereb Blood Flow Metab 2006;26:199-208.
8. Clancy RR, McGaurn SA, Goin JE, Hirtz DG, Norwood WI, Gaynor JW, et al. Allopurinol neurocardiac protection trial in infants undergoing heart surgery using deep hypothermic circulatory arrest. Pediatrics 2001; 108:61-70.
9. Asou H, Ono K, Uemura I, Sugawa M, Uyemura K. Axonal growth-related cell surface molecule, neurin-1, involved in neuron-glia interaction. J Neu-rosci Res 1996;45:571-587.
10. Himmelseher S, Pfenninger E, Georgieff M. Effects of basic fibroblast growth factor on hippocampal neurons after axonal injury. J Trauma 1997; 42:659-664.
11. Koeppen AH, Dickson AC, McEvoy JA. Te heterogeneous distribution of brain transferrin. J Neuropathol Exp Neurol 1995;54:395-403.
12. Houlgatte R, Mallat M, Brachet P, Prochiantz A. Secretion of nerve growth factor in cultures of glial cells and neurons derived from diferent regions of the mouse brain. J Neurosci Res 1989;24:143-152.
13. Rebouche CJ, Engel AG. Carnitine metabolism and deficiency syndromes. Mayo Clin Proc 1983;58:533-540.
14. Winter S, Jue K, Prochazka J, Francis P, Hamilton W, Linn L, et al. Te role of L-carnitine in pediatric cardiomyopathy. J Child Neurol 1995;10 Suppl 2:S45-51.
15. Fritz IB, Arrigoni-Martelli E. Sites of action of carnitine and its derivatives on the cardiovascular system: interactions with membranes. Trends Pharmacol Sci 1993;14:355-360.
16. Wainwright MS, Kohli R, Whitington PF, Chace DH. Carnitine treatment inhibits increases in cerebral carnitine esters and glutamate detected by mass spectrometry after hypoxia-ischemia in newborn rats. Stroke 2006; 37:524-530.
17. Matsuishi T, Stumpf DA, Seliem M, Eguren LA, Chrislip K. Propionate mitochondrial toxicity in liver and skeletal muscle: acyl CoA levels. Biochem Med Metab Biol 1991;45:244-253.
18. Pande SV, Blanchaer MC. Reversible inhibition of mitochondrial adenosine diphosphate phosphorylation by long chain acyl coenzyme A esters. J Biol Chem 197;246:402-411.
19. Loster H, Keller T, Grommisch J, Grunder W. Efects of L-carnitine and its acetyl and propionyl esters on ATP and PCr levels of isolated rat hearts perfused without fatty acids and investigated by means of 31P-NMR spectroscopy. Mol Cell Biochem 1999;200:93-102.
20. Wang D, Xia Y, Buja LM, McMillin JB. The liver isoform of carnitine palmitoyltransferase I is activated in neonatal rat cardiac myocytes by hypoxia. Mol Cell Biochem 1998;180:163-170.
21. Iijima T, Mishima T, Akagawa K, Iwao Y. Neuroprotective efect of pro-pofol on necrosis and apoptosis following oxygen-glucose deprivation: relationship between mitochondrial membrane potential and mode of death. Brain Res 2006;1099:25-32.
22. Slivka A, Silbersweig D, Pulsinelli W. Carnitine treatment for stroke in rats. Stroke 1990;21:808-811.
23. Shenk JC, Liu J, Fischbach K, Xu K, Puchowicz M, Obrenovich ME, et al. The effect of acetyl-L-carnitine and R-alpha-lipoic acid treatment in ApoE4 mouse as a model of human Alzheimer's disease. J Neurol Sci 2009;283:199-206.
24. Zou X, Sadovova N, Patterson TA, Divine RL, Hotchkiss CE, Ali SF, et al. Te efects of L-carnitine on the combination of, inhalation anesthetic-induced developmental, neuronal apoptosis in the rat frontal cortex. Neu-roscience 2008;151:1053-1065.
25. Athanassakis I, Zarif I, Evangeliou A, Vassiliadis S. L-carnitine accelerates the in vitro regeneration of neural network from adult murine brain cells. Brain Res 2002;932:70-78.
26. Loster H, Bohm U. L-carnitine reduces malondialdehyde concentrations in isolated rat hearts in dependence on perfusion conditions. Mol Cell Biochem 2001;217:83-90.
27. Long J, Gao F, Tong L, Cotman CW, Ames BN, Liu J. Mitochondrial decay in the brains of old rats: ameliorating efect of alpha-lipoic acid and acetyl-L-carnitine. Neurochem Res 2009;34:755-763.
28. Wainwright MS, Mannix MK, Brown J, Stumpf DA. L-carnitine reduces brain injury after hypoxia-ischemia in newborn rats. Pediatr Res 2003;54: 688-695.
29. Hurtado O, Lizasoain I, Fernandez-Tome P, Alvarez-Barrientos A, Leza JC, Lorenzo P, et al. TACE/ADAM17-TNF-alpha pathway in rat cortical cultures after exposure to oxygen-glucose deprivation or glutamate. J Cereb Blood Flow Metab 2002;22:576-585.
30. Sauvageot CM, Stiles CD. Molecular mechanisms controlling cortical gliogenesis. Curr Opin Neurobiol 2002;12:244-249.
31. Banker GA. Trophic interactions between astroglial cells and hippocampal neurons in culture. Science 1980;209:809-810.
32. Mattson MP, Barger SW, Begley JG, Mark RJ. Calcium, free radicals, and excitotoxic neuronal death in primary cell culture. Methods Cell Biol 1995;46:187-216.
33. Brewer GJ, Torricelli JR, Evege EK, Price PJ. Optimized survival of hip-pocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination. J Neurosci Res 1993;35:567-576.
34. Romera C, Hurtado O, Botella SH, Lizasoain I, Cardenas A, Fernandez-Tome P, et al. In vitro ischemic tolerance involves upregulation of glutamate transport partly mediated by the TACE/ADAM17-tumor necrosis factor-alpha pathway. J Neurosci 2004;24:1350-1357.
35. De Cristobal J, Cardenas A, Lizasoain I, Leza JC, Fernandez-Tome P, Lorenzo P, et al. Inhibition of glutamate release via recovery of ATP levels accounts for a neuroprotective effect of aspirin in rat cortical neurons exposed to oxygen-glucose deprivation. Stroke 2002;33:261-267.
36. Bank HL. Rapid assessment of islet viability with acridine orange and propidium iodide. In Vitro Cell Dev Biol 1988;24:266-273.
37. Meng XF, Zou XJ, Peng B, Shi J, Guan XM, Zhang C. Inhibition of ethanol-induced toxicity by tanshinone IIA in PC12 cells. Acta Pharmacol Sin 2006;27:659-664.
38. Gao F, Yi J, Yuan JQ, Shi GY, Tang XM. Te cell cycle related apoptotic susceptibility to arsenic trioxide is associated with the level of reactive oxygen species. Cell Res 2004;14:81-85.
39. Gwag BJ, Lobner D, Koh JY, Wie MB, Choi DW. Blockade of glutamate receptors unmasks neuronal apoptosis after oxygen-glucose deprivation in vitro. Neuroscience 1995;68:615-619.
40. He F, Wu LX, Liu FY, Yang LJ, Zhang Y, Zhang HF, et al. Protection of hepatocyte growth factor on neurons subjected to oxygen-glucose depriva-tion/reperfusion. Sheng Li Xue Bao 2008;60:235-242.
41. Tastekin A, Gepdiremen A, Ors R, Buyukokuroglu ME, Halici Z. Protec tive efect of L-carnitine against bilirubin-induced neuronal cell death. Brain Dev 2006;28:436-439.
42. Grojean S, Koziel V, Vert P, Daval JL. Bilirubin induces apoptosis via activation of NMDA receptors in developing rat brain neurons. Exp Neurol 2000;166:334-341.
43. Onem G, Aral E, Enli Y, Oguz EO, Coskun E, Aybek H, et al. Neuropro-tective effects of L-carnitine and vitamin E alone or in combination against ischemia-reperfusion injury in rats. J Surg Res 2006;131:124-130.
44. Burlina AP, Sershen H, Debler EA, Lajtha A. Uptake of acetyl-L-carnitine in the brain. Neurochem Res 1989;14:489-493.
45. Kim CS, Roe CR. Maternal and fetal tissue distribution of L-carnitine in pregnant mice: low accumulation in the brain. Fundam Appl Toxicol 1992;19:222-227.
46. Rebouche CJ. Carnitine function and requirements during the life cycle. FASEB J 1992;6:3379-3386.
47. Steiber AL, Weatherspoon LJ, Spry L, Davis AT. Serum carnitine concentrations correlated to clinical outcome parameters in chronic hemodialysis patients. Clin Nutr 2004;23:27-34.
48. De Vivo DC, Bohan TP, Coulter DL, Dreifuss FE, Greenwood RS, Nordli DR Jr, et al. L-carnitine supplementation in childhood epilepsy: current perspectives. Epilepsia 1998;39:1216-1225.
49. Pons R, De Vivo DC. Primary and secondary carnitine defciency syndromes. J Child Neurol 1995;10 Suppl 2:S8-24.
50. O'Donnell J, Finer NN, Rich W, Barshop BA, Barrington KJ. Role of L-carnitine in apnea of prematurity: a randomized, controlled trial. Pediatrics 2002;109:622-626.