In vitro and in vivo antioxidant activity of alfalfa (Medicago sativa L.) on carbon tetrachloride intoxicated rats

American Journal of Chinese Medicine

Volumn 40, Issue 4, 2012, Pages 779-793

  Al Dosari, Mohammed S ^a  

^a KING SAUD UNIVERSITY   (Saudi Arabia)

Author keywords

Alfalfa; Hepatoprotection; Lipid Peroxidation; Medicago sativa; Oxidative Stress

Indexed keywords

1,1 DIPHENYL 2 PICRYLHYDRAZYL; ALANINE AMINOTRANSFERASE; ALFALFA EXTRACT; ALKALINE PHOSPHATASE; ASPARTATE AMINOTRANSFERASE; BETA CAROTENE; BILIRUBIN; CARBON TETRACHLORIDE; LINOLEIC ACID; LIPID; LIVER ENZYME; MALONALDEHYDE; NON PROTEIN SULFHYDRYL; PLANT EXTRACT; PROTEIN; RUTOSIDE; SILYMARIN; UNCLASSIFIED DRUG;

ALANINE AMINOTRANSFERASE BLOOD LEVEL; ALFALFA; ALKALINE PHOSPHATASE BLOOD LEVEL; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIOXIDANT ACTIVITY; AQUEOUS SOLUTION; ARTICLE; ASPARTATE AMINOTRANSFERASE BLOOD LEVEL; BILIRUBIN BLOOD LEVEL; BLOOD SAMPLING; CLINICAL ASSESSMENT; CONTROLLED STUDY; DRUG EFFICACY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HISTOPATHOLOGY; IN VITRO STUDY; IN VIVO STUDY; LIPID ANALYSIS; LIPID BLOOD LEVEL; LIPID PEROXIDATION; LIVER; LIVER INJURY; LIVER PROTECTION; LYOPHILISATE; MALE; NONHUMAN; OXIDATIVE STRESS; PROTEIN BLOOD LEVEL; RAT; RISK REDUCTION; SCAVENGING SYSTEM;

ANIMALS; ANTIOXIDANTS; CARBON TETRACHLORIDE POISONING; LIPID PEROXIDATION; LIPIDS; MALE; MALONDIALDEHYDE; MEDICAGO SATIVA; PLANT EXTRACTS; RATS; RATS, WISTAR; SILYMARIN;

EID: 84864196745     PISSN: 0192415X     EISSN: None     Source Type: Journal    
DOI: 10.1142/S0192415X12500589     Document Type: Article

References (52)

1. Ahmad, S., S. Yousuf and T. Ishrat. Effect of dietary sesame oil as antioxidant on brain hippocampus of rat in focal cerebral ischemia. Life Sci. 79: 1921-1928, 2006. (Pubitemid 44508638)
2. Al-Dosari, M., S. Alqasoumi, M. Ahmed, M. Ahmed, M.N. Ansari and S. Rafatullah. Effect of Beta vulgaris L. on cholesterol rich diet-induced hypercholesterolemia in rats. Farmacia 59: 669-678, 2011.
3. Al-Dosari, M.S. Antioxidant and protective effects of spinach (Spinacia oleracea L.) leaves against carbon tetrachloride-induced liver injury. Clin. Exptl. Med. J. 4: 129-140, 2010.
4. Al-Howiriny, T. Evaluation of hepatoprotective activity of broccoli Brassica oleracea in rats. Hung. Med. J. 2: 145-156, 2008a.
5. Al-Howiriny, T.A. Protective effect of "purslane" on rat liver injury induced by carbon tetrachloride. Saudi Pharm. J. 16: 239-244, 2008b
6. Alison, M.G. and R.F. Peter. Pancreatic and extrapancreatic effects of the traditional antidiabetic plant Medicago sativa. Br. J. Nutr. 78: 325-334, 1997.
7. Alqasoumi, S., M. Al-Yahya, T. Al-Howiriny and S. Rafatullah. Gastroprotective effect of radish "Raphanus sativus" L. on experimental gastric ulcer models in rats. Farmacia 46: 204-214, 2008. (Pubitemid 351611720)
8. Asgary, S., J.M. Moshtaghian, M. Hosseini and H. Siadat. Effects of alfalfa on lipoproteins and fatty streak formation in hypercholesterolemic rabbits. Pak. J. Pharm. Sci. 21: 460-464, 2008.
9. Balasubramanian, R., J. Balasundaram, K. Subramanian and M. Narayanan. Effect of dried fruits of Carica papaya Linn. on hepatotoxicity. Biol. Pharm. Bull. 25: 1645-1646, 2002.
10. Bora, K.S. and A. Sharma. Bioactivity-directed separation of an anxiolytic fraction from Medicagosativa Linn. J. Pharm. Biomed. Sci. 8(8): 1-4, 2011
11. Bora, K.S. and A. Sharma. Evaluation of antioxidant and cerebroprotective effect of Medicago sativa Linn. against ischemia and reperfusion insult. Evid. Based Complement. Alternat. Med. 2011: 792167, 2011b
12. Brand, W.W., H.E. Cuvelier and C. Berset. Use of a free radical method to evaluate antioxidant activity. Food Sci. Technol. 82: 25-30, 1995.
13. Burstein, M. and H.R. Scholnick. Lipoprotein-polyanion-metal interaction. Adv. Lipid Res. 11: 67-108, 1973.
14. Cabre, M., J. Camps, J.L. Patenain, N. Ferre and J. Joven. Time-course of change in hepatic peroxidation and glutathione metabolism in rats with carbon tetrachloride-induced cirrhosis. Clin. Exp. Pharmacol. Physiol. 27: 694-699, 2000. (Pubitemid 30666190)
15. Chaudhary, G.D., P. Kamboj, I. Singh and A.N. Kalia. Herbs as liver savers - a review. Indian J. Nat. Prod. Resourc. 1(4): 397-408, 2010.
16. Culling, C.F. Handbook of Histopathological and Histochemical Techniques, 3rd edn. Butterworth and Co., London, 1974, p. 37.
17. Dandiya, P.C. and H. Collumbine. Studies on Acoru scalamus: Pharmacological actions of essential oil. J. Pharmacol. Exp. Ther. 125: 353-359, 1959.
18. Demacher, P.N.M. and A.G.M. Hijamaus. A study of the use of polyethylene glycol in estimating cholesterol. Clin. Chem. 26: 1775-1778, 1980.
19. Di Carlo, G., N. Mascolo, A.A. Izzo and F. Capasso. Flavonoids: Old and new aspects of a class of natural therapeutic drugs. Life Sci. 65: 337-342, 1999.
20. Fiala, S., A.E. Fiala. and S. Dixon. Gamma glutamyl transpeptidase in transplantable chemically induced rat hepatomas and spontaneous mouse hepatomas. J. Natl. Cancer Inst. 48: 1393-1409, 1972.
21. Foster, L.B. and R.T. Dunn. Stable reagents for the determination of serum triglycerides by a colorimetric Hantzch condensation method. Clin. Chem. 19: 338-340, 1973.
22. Haldar, P.K., S. Bera, S.P. Panda, S. Bhattacharya, N.C. Chaulya and A. Mukherjee. Hepatoprotective activity of Cyperus tegetum rhizome against paracetamol-induced liver damage in rats. J. Complement. Int. Med. 8: 1398, 2011.
23. Janero, D.R. Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Rad. Biol. Med. 9: 515-540, 1990.
24. Kaplowitz, N., T.T.Y. Aw, F.R. Simon and A. Stolz. Drug induced hepatotoxicity. Ann. Int. Med. 104: 826-839, 1986.
25. Khader, M.E., K.A. Mahdy, K.A. El-Shamy, F.A. Morsy, S.R. El-Zayat and A.A. Abd-Allah. Antioxidant activity and hepatoprotective potential of black seed, honey and silymarin on experimental liver injuries induced by CCl4 in rats. J. Appl. Sci. 7: 3909-3917, 2007.
26. Khaleel, A.E., Z. Mohamed, G.A.E. Shohda, S.H. Mohamed and E.A. Sattar. Study of hypocholesterolemic and antiatherosclerotic properties of Medicago sativa L. cultivated in Egypt. J. Food Drug Anal. 13: 212-218, 2005. (Pubitemid 41684312)
27. King, E.J. and A.R. Armstrong. Calcium, phosphorus and phosphate. In: H. Varley (ed.), Practical Clinical Biochemistry. CBS Publishers, New Delhi, 1988, p. 458.
28. Krishnakumar, N.M., P.G. Latha, S.R. Suja, V.J. Shine, S. Shyamal, G.I. Ajuna, S. Sini, S. Pradeep, P. Shikha, P.K. Somasekharan Unni and S. Rajasekharan. Hepatoprotective effect of Hibiscus hispidissimus Griffith, ethanolic extract in paracetamol and CCl4 induced hepatotoxicity in Wistar rats. Indian J. Exp. Biol. 46: 653-659, 2008.
29. Mothana, R.A.A., M.S. Al-Said, A.J. Al-Rehaily, T.M. Thabet, N.A. Awad, M. Lalk and U. Lindequist. Anti-inflammatory, antinociceptive, antipyretic and antioxidant activities and phenolic constituents from Loranthu sregularis Steud.ex Sprague. Food Chem. 130: 344-349, 2012.
30. Murphy, R. Lotus Materia Medica, 2nd ed. 2004, p. 89.
31. Nabavi, S.F., A.H. Moghaddam, S. Eslami and S.M. Nabavi. Protective effects of curcumin against sodium fluoride-induced toxicity in rat kidneys. Biol. Trace Elem. Res. 145: 369-374, 2012a
32. Nabavi, S.F., S.M. Nabavi, F. Abolhasani, A.H. Moghaddam and S. Eslami. Cytoprotective effects of curcumin on sodium fluoride-induced intoxication in rat erythrocytes. Bull. Environ. Contam. Toxicol. 88: 486-490, 2012b
33. Nabavi, S.M., F.S. Nabavi, S. Eslami, A.H. Moghaddam. In vivo protective effects of quercetin against sodium fluoride-induced oxidative stress in the hepatic tissue. Food Chem. 132: 931-935, 2012c.
34. OECD Guidelines for testing chemicals, Guidelines 423, acute oral toxicity: Acute toxic class method. Paris, 2001.
35. Pandey, A., P. Bigoniya, V. Raj and K.K. Patel. Pharmacological screening of Coriandrum sativum Linn. for hepatoprotective activity. J. Pharm. Bioallied Sci. 3: 435-441, 2011.
36. Rafatullah, S., J.S. Mossa, M. Al-Yahya and A.M. Ageel. Hepatoprotective and safety evaluation studies on Sarsaparilla. Int. J. Pharmacol. 29: 296-301, 1991.
37. Recknagel, R.D., E.A. Glende, J.A. Dolak and R.L. Wailer. Mechanism of carbon tetrachloride toxicity. Pharmacol. Ther. 43: 139-154, 1989.
38. Recknagel, R.O. Carbon tetrachloride hepatotoxicity. Pharmacol. Rev. 19: 145-208, 1967.
39. Recknagel, R.O., E.A. Glende, G. Ugazio, R.R. Koch and S. Srinivasan. New data in support of the lipid peroxidation theory of carbon tetrachloride liver injury, Israeli J. Med. Sci. 10: 301, 1974.
40. Reilly, P. Clinical application - Medicago sativa extracts. J. Naturopath. Med. 1: 455-460, 1989.
41. Reitman, S.A. and A. Frankel. A colorimetric method for the determination of serum glutamic oxaloaceric acid and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 28: 56-63, 1957.
42. Sedlak, J. and R.H. Lindasy. Estimation of total protein bound and nonprotein sulfhydryl group in tissue with Ellman's reagents. Anal. Biochem. 25: 192-205, 1968.
43. Senthikumar, K.T.M., B. Rajkapoor and S. Kavimani. Protective effect of Enicostemma littorale against CCl4 induced hepatic damage in rats. Pharm. Biol. 43: 485-487, 2005. (Pubitemid 41291334)
44. Singh, B., A.K. Saxena, B.K. Chandran, K.K. Anand and O.P. Suri. Hepatoprotective activity of verbenalin on experimental liver damage in rodents. Fitoterapia 69: 135-141, 1998.
45. Socha, P., J. Rujner and J. Socha. The role of oxygen radicals and their antioxidants in pathogenesis of chronic hepatitis. Ped. Pol. 14: 139-144, 1992.
46. Stiehl, A. Hyperbilirubinamie bei Lebererkrankungen. Fortschr. Med. 100: 842-845, 1982. (Pubitemid 12068192)
47. Stuart, M. The Encyclopaedia of Herb and Herbalism. Orbis Publishing, London, 1979, p. 220.
48. Subramonian, A. and P. Pushpangadan. Development of phytomedicine for liver diseases, Indian J. Pharmacol. 31: 166-175, 1999. (Pubitemid 29317731)
49. Thomas, J.A., B. Poland and R. Honzatko. Protein sulfhydryls and their role in the antioxidant function of protein S-thiolation. Arch. Biochem. Biophys. 319: 1-9, 1995.
50. Utley, H.G., F. Bernheim and P. Hochstein. Effect of sulfhydryl reagents on peroxidation in microsomes. Arch. Biochem. Biophys. 118: 29-32, 1967.
51. Videla, L.A. and A. Valenzula. Alcohol ingestion, liver glutathione and lipid peroxidation, metabolic interaction and parameters implication. Life Sci. 31: 2395-2407, 1982. (Pubitemid 13168687)
52. Williamson, E.M., D.T. Okpako and F.J. Evans. Selection, Preparation and Pharmacological Evaluation of Medicinal Plant Material. John Wiley, England, 1996.