Combined HILIC-ELSD/ESI-MS n enables the separation, identification and quantification of sugar beet pectin derived oligomers

Carbohydrate Polymers

Volumn 90, Issue 1, 2012, Pages 41-48

  Remoroza, C ^a   Cord Landwehr, S ^b   Leijdekkers, A G M ^a   Moerschbacher, B M ^b   Schols, H A ^a   Gruppen, H ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

ELSD; Endo polygalacturonase; HILIC; Mass spectrometry; Pectin lyase; Sugar beet pectin

Indexed keywords

ACETYL GROUPS; ACETYLATED SUGARS; COMBINED ACTIONS; DEGRADING ENZYMES; ELECTROSPRAY IONIZATION ION TRAP MASS SPECTROMETRY; ELSD; ENDO-POLYGALACTURONASE; EVAPORATIVE LIGHT SCATTERING DETECTION; GALACTURONIC ACIDS; HILIC; HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHIES; METHYL ESTERS; PECTIN METHYL ESTERASE; PECTIN-LYASE;

CHROMATOGRAPHY; DEGRADATION; ELECTROSPRAY IONIZATION; MASS SPECTROMETRY; SEPARATION; SUGAR BEETS;

OLIGOMERS;

BETA VULGARIS SUBSP. VULGARIS;

CARBOXYLESTERASE; PECTIN; PECTIN LYASE; PECTINESTERASE; POLYGALACTURONASE; POLYSACCHARIDE LYASE;

ASPERGILLUS; BEET; CARBOHYDRATE ANALYSIS; CHEMICAL PHENOMENA; CHEMISTRY; CHRYSOSPORIUM; ELECTROSPRAY MASS SPECTROMETRY; ENZYMOLOGY; HYDROLYSIS; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; METABOLISM; MOLECULAR GENETICS;

ASPERGILLUS; BETA VULGARIS; CARBOHYDRATE SEQUENCE; CARBOXYLIC ESTER HYDROLASES; CHROMATOGRAPHY, LIQUID; CHRYSOSPORIUM; HYDROLYSIS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; MOLECULAR SEQUENCE DATA; PECTINS; POLYGALACTURONASE; POLYSACCHARIDE-LYASES; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 84864150513     PISSN: 01448617     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.carbpol.2012.04.058     Document Type: Article

References (33)

1. G. Beldman, M.J.F. Searle-van Leeuwen, G.A. De Ruiter, H.A. Siliha, and A.G.J. Voragen Degradation of arabinans by arabinanases from Aspergillus aculeatus and Aspergillus niger Carbohydrate Polymers 20 3 1993 159 168
2. E. Bonnin, E. Dolo, A. Le Goff, and J.-F. Thibault Characterisation of pectin subunits released by an optimised combination of enzymes Carbohydrate Research 337 18 2002 1687 1696
3. H.C. Buchholt, T.M.I.E. Christensen, B. Fallesen, M.C. Ralet, and J.F. Thibault Preparation and properties of enzymatically and chemically modified sugar beet pectins Carbohydrate Polymers 58 2 2004 149 161
4. P.J.H. Daas, P.W. Arisz, H.A. Schols, G.A. De Ruiter, and A.G.J. Voragen Analysis of partially methyl-esterified galacturonic acid oligomers by high-performance anion-exchange chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Analytical Biochemistry 257 2 1998 195 202
5. P.J.H. Daas, K. Meyer-Hansen, H.A. Schols, G.A. De Ruiter, and A.G.J. Voragen Investigation of the non-esterified galacturonic acid distribution in pectin with endopolygalacturonase Carbohydrate Research 318 1-4 1999 135 145
6. K. Decroos, J.P. Vincken, L. Heng, R. Bakker, H. Gruppen, and W. Verstraete Simultaneous quantification of differently glycosylated, acetylated, and 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one-conjugated soyasaponins using reversed-phase high-performance liquid chromatography with evaporative light scattering detection Journal of Chromatography A 1072 2 2005 185 193
7. B. Domon, and C.E. Costello A systematic nomenclature for carbohydrate fragmentations in FAB-MS/MS spectra of glycoconjugates Glycoconjugate Journal 5 4 1988 397 409
8. F. Guillon, J.-F. Thibault, F.M. Rombouts, A.G.J. Voragen, and W. Pilnik Enzymic hydrolysis of the hairy fragments of sugar-beet pectins Carbohydrate Research 190 1 1989 97 108
9. S.E. Guillotin, J. Van Kampen, P. Boulenguer, H.A. Schols, and A.G.J. Voragen Degree of blockiness of amide groups as indicator for difference in physical behavior of amidated pectins Biopolymers 82 1 2006 29 37
10. S.E. Guillotin, A. Van Loey, P. Boulenguer, H.A. Schols, and A.G.J. Voragen Rapid HPLC method to screen pectins for heterogeneity in methyl-esterification and amidation Food Hydrocolloids 21 1 2007 85 91
11. J.A.M. Harmsen, M.A. Kusters-van Someren, and J. Visser Cloning and expression of a second Aspergillus niger pectin lyase gene (pelA): Indications of a pectin lyase gene family in A. niger Current Genetics 18 2 1990 161 166
12. R. Körner, G. Limberg, T.M.I.E. Christensen, J.D. Mikkelsen, and P. Roepstorff Sequencing of partially methyl-esterified oligogalacturonates by tandem mass spectrometry and its use to determine pectinase specificities Analytical Chemistry 71 7 1999 1421 1427
13. S. Kühnel, S.W.A. Hinz, L. Pouvreau, J. Wery, H.A. Schols, and H. Gruppen Chrysosporium lucknowense arabinohydrolases effectively degrade sugar beet arabinan Bioresource Technology 101 21 2010 8300 8307
14. S. Kuhnel, H.A. Schols, and H. Gruppen Aiming for the complete utilization of sugar-beet pulp: Examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion Biotechnology for Biofuels 4 1 2011 14
15. A.G.M. Leijdekkers, M.G. Sanders, H.A. Schols, and H. Gruppen Characterizing plant cell wall derived oligosaccharides using hydrophilic interaction chromatography with mass spectrometry detection Journal of Chromatography A 1218 51 2011 9227 9235
16. G. Limberg, R. Körner, H.C. Buchholt, T.M.I.E. Christensen, P. Roepstorff, and J.D. Mikkelsen Quantification of the amount of galacturonic acid residues in blocksequences in pectin homogalacturonan by enzymatic fingerprinting with exo- and endo-polygalacturonase II from Aspergillus niger Carbohydrate Research 327 3 2000 321 332
17. Y. Mengerink, H.C.J. De Man, and S. Van der Wal Use of an evaporative light scattering detector in reversed-phase high-performance liquid chromatography of oligomeric surfactants Journal of Chromatography 552 1-2 1991 593 604
18. M. Mutter, C.M.G.C. Renard, G. Beldman, H.A. Schols, and A.G.J. Voragen Mode of action of RG-hydrolase and RG-lyase toward rhamnogalacturonan oligomers. Characterization of degradation products using RG-rhamnohydrolase and RG-galacturonohydrolase Carbohydrate Research 311 3 1998 155 164
19. P.W. Needs, N.M. Rigby, I.J. Colquhoun, and S.G. Ring Conflicting evidence for non-methyl galacturonoyl esters in Daucus carota Phytochemistry 48 1 1998 71 77
20. A. Oosterveld, G. Beldman, H.A. Schols, and A.G.J. Voragen Arabinose and ferulic acid rich pectic polysaccharides extracted from sugar beet pulp Carbohydrate Research 288 0 1996 143 153
21. P. Perrone, C.M. Hewage, A.R. Thomson, K. Bailey, I.H. Sadler, and S.C. Fry Patterns of methyl and O-acetyl esterification in spinach pectins: New complexity Phytochemistry 60 1 2002 67 77
22. B. Quéméner, J.C. Cabrera Pino, M.-C. Ralet, E. Bonnin, and J.-F. Thibault Assignment of acetyl groups to O-2 and/or O-3 of pectic oligogalacturonides using negative electrospray ionization ion trap mass spectrometry Journal of Mass Spectrometry 38 6 2003 641 648
23. M.C. Ralet, J.C. Cabrera, E. Bonnin, B. Quéménér, P. Hellín, and J.F. Thibault Mapping sugar beet pectin acetylation pattern Phytochemistry 66 15 2005 1832 1843
24. M.C. Ralet, M.J. Crepeau, and E. Bonnin Evidence for a blockwise distribution of acetyl groups onto homogalacturonans from a commercial sugar beet (Beta vulgaris) pectin Phytochemistry 69 9 2008 1903 1909
25. H.A. Schols, M.A. Posthumus, and A.G.J. Voragen Structural features of hairy regions of pectins isolated from apple juice produced by the liquefaction process Carbohydrate Research 206 1 1990 117 129
26. R.R. Selvendran, J.F. March, and S.G. Ring Determination of aldoses and uronic acid content of vegetable fiber Analytical Biochemistry 96 2 1979 282 292
27. B.R. Thakur, R.K. Singh, A.K. Handa, and M.A. Rao Chemistry and uses of pectin - A review Critical Reviews in Food Science and Nutrition 37 1 1997 47 73
28. G.J.W.M. Van Alebeek, T.M.I.E. Christensen, H.A. Schols, J.D. Mikkelsen, and A.G.J. Voragen Mode of action of pectin lyase A of Aspergillus niger on differently C6-substituted oligogalacturonides Journal of Biological Chemistry 277 29 2002 25929 25936
29. G.J.W.M. Van Alebeek, K. Van Scherpenzeel, G. Beldman, H.A. Schols, and A.G.J. Voragen Partially esterified oligogalacturonides are the preferred substrates for pectin methylesterase of Aspergillus niger Biochemical Journal 372 1 2003 211 218
30. A.G.J. Voragen, G.J. Coenen, R.P. Verhoef, and H.A. Schols Pectin, a versatile polysaccharide present in plant cell walls Structural Chemistry 20 2 2009 263 275
31. A.G.J. Voragen, W. Pilnik, J.-F. Thibault, M.A.V. Axelos, and C.M.G.C. Renard Pectins A.M. Stephen, Food polysaccharides and their applications 1995 M. Dekker New York/Basel/Hong Kong 287 339
32. Y. Westphal, H.A. Schols, A.G.J. Voragen, and H. Gruppen Introducing porous graphitized carbon liquid chromatography with evaporative light scattering and mass spectrometry detection into cell wall oligosaccharide analysis Journal of Chromatography A 1217 5 2010 689 695
33. P.A. Williams, C. Sayers, C. Viebke, C. Senan, J. Mazoyer, and P. Boulenguer Elucidation of the emulsification properties of sugar beet pectin Journal of Agricultural and Food Chemistry 53 9 2005 3592 3597